Isolation of BAC DNA from bulk cultures
The amount of DNA required for fine analysis of recombinant BAC, including detailed restriction mapping, DNA sequencing or subcloning, is greater than can be pr
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Polyacrylamide gel electrophoresis purification of oligonucleotide experiment
The method detailed in this protocol for the purification of crude synthesized oligonucleotides using denaturing polyacrylamide gel electrophoresis is a modific
Application of yeast artificial chromosomes
Until the mid-1980s, there were no techniques for isolating, amplifying, and analyzing large sticky fragments of genomic DNA. Prior to that time, if a DNA fragm
Experiments on purification of radiolabeled oligonucleotides by ethanol precipitation method
If the radiolabeled oligonucleotide is to be used only as a hybridization probe, it is generally not necessary to completely remove the unadulterated radiolabel
Isolation of BAC DNA from small cultures
Small amounts of BAC DNA were prepared from 5 ml of BAC-transformed cell cultures.The DNA was prepared by alkaline lysis.The yield of BAC DNA can be as high as
Application of bacterial artificial chromosomes
The bacterial artificial chromosome (RAC ) is a synthetic vector based on the E. coli fertility factor (F). This experiment is based on the "Guide to Molecular
Exon capture and amplification
This protocol describes the method of exon amplification using the mammalian shuttle vector pSPL3 as an example, which is divided into the following five stages
Recovery of DNA from agarose and polyacrylamide gels (electroelution to dialysis bags)
This technique (McDonell et al., 1977) allows the recovery of double-stranded DNA from agarose or polyacrylamide gel slices over a wide range of molecular masse
Experiments on purification of radiolabeled oligonucleotides by Sep-Pak C18 column chromatography
This scheme describes a method to separate radiolabeled oligonucleotides from undoped radiolabel using the property of antiphase affinity of oligonucleotides fo