Screening of expression libraries constructed in λ phage vectors
In typical immunoscreening experiments, libraries of λ phage expression vector constructs should be spread on plates of E. coli strains that do not conta
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λ Experiments on spread plate culture of phages
Phages originate from the infection of a single bacterium by a single phage particle. The daughter virus particles synthesized after the first infection adsorb
DNA binding protein assay
This experiment was used to detect sequence-specific DNA-binding proteins in crude extracts.
Determination of DNA content in λ phage progeny and lysates by gel electrophoresis experiment
This program describes a method for rapid estimation of the DNA content in λ phage protospecies. The method can be used firstly to find out whether the y
Extraction of λ phage DNA from large-scale cultures with formamide
Instead of SDS/proteinase K, formamide can be used to remove the shell from purified phage particles. This method is not very efficient, but in a sense it is fa
Lysis of colonies and DNA binding assay to filter membranes
This protocol describes how to release DNA from a clone carrying a recombinant plasmid and immobilize it on a nitrocellulose filter or nylon membrane, a method
λ Alkaline phosphatase treatment assay of phage vector DNA
Removal of the terminal 5'-phosphate groups from the inner ends of the two arms of the λ phage effectively prevents self-association and reduces the back
Preparation of λ phage proto-species experiments with small quantities of liquid cultures
High-titer λ phage progenitors can be prepared from small quantities of liquid cultures. This method was first used by Leder et al. (1977). In general, i
Preparation of λ phage DNA by double restriction enzyme cleavage as a cloning vector
Replacement vectors (e.g., λ2001, λDASH, EMBL series, Charon 34, Charon 35, and Charon 40) contain a series of restriction sites that are aligned