Pyrophosphate sequencing
Pyrosequencing is a luminescence-based sequencing technology for the determination of pyrophosphate (PPi). The three key points of pyrosequencing are: (1) dATPa
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Mutagenesis of genomes using chemical mutagens
Currently, there are two main strategies for studying gene function using model mice: the gene-driven approach and the phenotype-driven approach. Gene knockout
DNA recombination in vitro
DNA recombination, also known as gene cloning, is the most basic technique in gene manipulation and the core technology of molecular biology.DNA recombination r
Isolation of RNA based on size
RNA samples may be denatured by treatment with formamide and by electrophoretic separation on gels containing formaldehyde, a method modified from Lehrachetal.
Determination of DNA concentration and purity
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In-situ notch panning technique
The in situ nick translation technique is also known as in situ nick translation. It was first used to study DNA replication in vitro, and has since been extend
Microbial Genome DNA Extraction
The yeast is first lysed using a solution containing leaving salts to ensure complete denaturation of the macromolecules. Afterwards, ethanol is added and the D
Cross-protection neutralization experiments
When an animal is infected by a virus, specific neutralizing antibodies are produced in the body and bind specifically to the corresponding virus particles, thu
Polyclonal antibody purification assay
The octanoic acid extraction method can be used to isolate and extract Ig from human, rabbit and mouse serum and McAb from hybridoma-induced ascites and culture