DNA synthesis assay with 3H-TdR doping assay
Cells were labeled with 3H -TdR and DNA was extracted to determine radioactivity levels by scintigraphy Source : Animal Cell Culture : A Guide to Basic Techniqu
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T lymphocyte transformation assay
When T cells are stimulated by non-specific mitogens (e.g. PHA, ConA) during in vitro culture, they may be transformed into lymphoblastoid cells with increased
Apoptosis detection by AO staining (Amino Orange Stain)
The dye stains the cells simultaneously, providing a better method for identifying apoptotic and necrotic cells
PFA fixation assay of cells
When pure formaldehyde is dissolved in aqueous solution, it automatically polymerizes into long-chain paraformaldehyde. Its length varies and the polymerization
Three-dimensional cell culture experiment
Three-dimensional (3D) cell culture refers to the co-culture of carriers with different materials of three-dimensional structure and different kinds of cells in
Live staining experiments of the vesicular lineage in cells
The vesicular lineage live staining assay in cells takes advantage of the property that neutral red (neutrol red) is a specific live stain for the vesicular lin
Cell recovery assay
Cell resuscitation is the process of thawing cells frozen in liquid nitrogen and then re-culturing them to restore cell growth.
Induced differentiation of human embryonic stem cells
Embryonic stem cells are cells with a self-renewal and totipotent differentiation ability, capable of developing and differentiating into all tissues and organs
Cell membrane permeability assay experiment
The cell membrane is the barrier for the exchange of substances between the cell and the external environment, and is a semi-permeable membrane that can selecti