Reverse transcriptase in situ PCR assay
This section describes an abbreviated method for in situ PCR, specifically reverse transcriptase in situ PCR. Each of these experimental procedures is discussed
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High-quality DNA purification assay
The application of a modified version of the salt precipitation method of Miller et al. (1988) for DNA purification, which does not use phenol or chloroform, is
Primer design and end-labeling experiments
Systematic analysis of SSCP parsing capabilities and limitations revealed a significant correlation between sensitivity and fragment length. The highest detecti
Multivariate real-time PCR experiment
In multiplex real-time PCR, pairs of primers with different markers are added to a single reaction tube, allowing simultaneous analysis of several different gen
Gel electrophoresis experiment
When optimizing the experimental conditions, the PCR products should be separated on gels containing glycerol and without glycerol at the same time and the resu
Large-scale PCR for cDNA microarrays
This protocol describes the experimental procedure to make cDNA microarrays by PCR amplification of products. This experiment was derived from PCR Lab Guide (Se
Reverse transcription and DNA amplification experiments with activated heat-stable DNA polymerase
This experiment introduces activated heat-stable DNA polymerase for reverse transcription and DNA amplification. This experiment was derived from PCR Laboratory
PCR amplification of differential DNA
In the reactions described in this protocol, differential DNA is selectively amplified. There should be at least 4 reactions per experiment: 1) ablated test DNA
PCR-based DNA blotting experiments
For high-throughput screening, it is recommended to use 96- or 384-well PCR plates available from several thermal cycler manufacturers, or to use individual tub