Determination of tissue level metallothionein by cadmium-hemoglobin method
Metallothionein (MT) is a metal- and sulfur-rich inducible protein, which can be induced by certain metals (Bi, Zn, Cu, etc.), hormones (glucocorticoids), cytot
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Molecular exclusion high performance liquid chromatography experiments
Molecular exclusion chromatography, also known as gel chromatography, is the use of different molecular sizes of the separated substances lead to different degr
Expression and purification experiments of glutathione S-transferase fusion protein
The pGEX vectors can be used to express and purify peptides (including short peptides) used as immunogens and biochemical and biological reagents, or to constru
Extraction of myocardial sarcoplasmic reticulum calcium-triphosphate adenosine monophosphatase
Cardiac sarcoplasmic reticulum calcium-ATPase (SERCA2a) plays a major role in the regulation of calcium homeostasis in cardiomyocytes, and studies on the gene t
Immunofluorescence localization experiments of nuclear proteins
Immunofluorescence localization of nuclear proteins can be applied to (1) localize intracellular distribution of nuclear proteins and (2) viral studies.
Determination of the activity of myocardial sarcoplasmic reticulum calcium-triphosphate adenosine monophosphatase
Cardiac sarcoplasmic reticulum calcium-ATPase (SERCA2a) plays a major role in the regulation of calcium homeostasis in cardiomyocytes, and studies on the gene t
Protein recovery assay
Recovery test is a type of "control test". When the analyzed sample is complex and not completely clear, a known amount of the measured component is added to th
Capture experiments of interacting proteins
Capture assays for interacting proteins are subjected to two sequential bulk yeast plate screening processes. Saccharomyces cerevisiae contains the lexA fusion
Probe synthesis experiments
A probe is a small fragment of single-stranded DNA or RNA (approximately 20 to 500 bp) that is used to detect a nucleic acid sequence complementary to it. Doubl