UV spectra of pure proteins A280 nm/A260 nm Experimental
A relatively simple and effective measure of protein purity is obtained by determining the ultraviolet (UV) spectra of purified proteins. Even if sodium dodecyl
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Elution from PEI precipitation σ32 and RNA polymerase assay
This experiment describes the amount of salt needed to elute σ32 and RNA polymerase from PEI precipitation. This experiment is from the Protein Purificati
Isolation and purification of animal genomic DNA
The purpose of this experiment is to master the basic principles and methods of mass preparation of animal genomic DNA by salt solubilization method, according
Expression and purification experiments of LacZ and trpE fusion proteins
Fusion proteins are generally used as antigens to stimulate antibody production and in many cases for biochemical analysis. Two widely used E. coli expression s
Extraction and activity of ATPase from cardiac myofibrillar fibers
Cardiac myofibrillar ATPase is Ca2+, Mg2+ ATPase, which breaks down ATP into ADP and Pi and releases energy to provide myofibrillar contraction needs. Myofibril
Purification of PPO crude enzyme solution
The purification of PPO can be applied to (1) the study of PPO nutritional mechanisms and (2) PPO physiological mechanisms and regulation.
Reversible staining of proteins on PVDF membranes
Reversible staining of proteins on PVDF membranes can be used for: confirming the transfer of proteins to PVDF membranes during Western hybridization.
Determination of substrate binding spectra to hepatic cytochrome P-450
The hepatic cytochrome P450 system plays an important role in the metabolism of drugs by converting them from hydrophobic to more easily excreted hydrophilic fo
Heparanase Lowry colorimetric assay
The Lowry colorimetric method is one of the most sensitive methods for protein determination. The color development principle of this method is the same as that