Recombinant Human ASXL2 Protein: Research Guide & Protocol

Recombinant Human ASXL2 Protein is a critical epigenetic regulatory factor involved in Polycomb-group complex assembly and histone modification, widely used in chromatin biology and cancer epigenetics research.

Product Name

Recombinant Human ASXL2 Protein

SKU

rp376570

Protein Origin

Human (Homo sapiens)

Protein Class

Recombinant epigenetic regulatory protein

Primary Application

Chromatin biology, epigenetic research, protein–protein interaction studies

Storage Recommendation

Avoid repeated freeze–thaw cycles; store at recommended temperature per datasheet

Background: ASXL2 Biology and Functional Significance

ASXL2 (Additional Sex Combs Like 2) is a member of the ASXL protein family, which plays essential roles in epigenetic regulation through interactions with Polycomb repressive complexes (PRCs) and trithorax group proteins. ASXL2 functions as a scaffold component facilitating histone H2A ubiquitination and modulation of H3K27 methylation states, thereby influencing gene silencing and activation programs critical for development and differentiation.

Dysregulation of ASXL2 has been associated with hematological malignancies and developmental disorders, making it a protein of significant interest in cancer epigenetics and translational research. The recombinant human form of ASXL2 enables investigators to study its biochemical activities, binding partners, and regulatory mechanisms in controlled in vitro settings.

Reconstitution and Handling Protocol

Proper handling of Recombinant Human ASXL2 Protein is essential to preserve its structural integrity and biological activity. Follow the guidelines below to ensure optimal experimental outcomes:

• Reconstitution: Briefly centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile, ultrapure water or a manufacturer-recommended buffer. Allow the solution to equilibrate at room temperature for several minutes before gently mixing. Do not vortex.
• Concentration: Prepare working aliquots at concentrations appropriate for your assay. Refer to the product datasheet (absea#PP-10118) for specific concentration recommendations.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize degradation from repeated freeze–thaw cycles.
• Storage: Store reconstituted protein according to datasheet instructions. Lyophilized material should be stored as directed until use.
• Carrier Protein: Addition of a carrier protein such as BSA (0.1–0.5%) may enhance stability during long-term storage; confirm compatibility with your downstream application before use.

Always work on ice when handling the protein and use low-protein-binding tubes to minimize adsorption losses.

Applications in Epigenetic and Chromatin Research

Recombinant Human ASXL2 Protein supports a broad range of research applications in epigenetics and chromatin biology:

• Protein–Protein Interaction Studies: Co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) to characterize ASXL2 binding partners within PRC1/PRC2 complexes.
• Enzymatic Activity Assays: In vitro histone ubiquitination and deubiquitination assays to dissect ASXL2-dependent chromatin modification pathways.
• Structural Biology: Use as a component for cryo-EM or X-ray crystallography studies examining higher-order chromatin regulatory complexes.
• Antibody Validation: Positive control antigen for western blot, ELISA, and immunofluorescence assay development and antibody specificity testing.
• Cell-Free Transcription Assays: Reconstitution of chromatin regulatory circuits in cell-free systems to study transcriptional repression mechanisms.
• Drug Discovery: Target protein for small-molecule screening campaigns aimed at disrupting oncogenic ASXL2 interactions in hematologic cancers.

Quality Control and Experimental Considerations

To obtain reproducible results with Recombinant Human ASXL2 Protein, researchers should incorporate the following quality control measures:

• Purity Verification: Confirm protein purity by SDS-PAGE prior to use in functional assays. Expected purity and molecular weight information is provided in the product datasheet.
• Activity Testing: Where applicable, validate protein activity using a functional assay relevant to your experimental system before committing to large-scale experiments.
• Batch-to-Batch Consistency: Record lot numbers for all experiments. If switching lots, run a side-by-side comparison to confirm equivalent performance.
• Endotoxin Levels: For cell-based assays, confirm endotoxin levels are within acceptable limits as specified on the certificate of analysis (COA).
• Negative Controls: Always include appropriate negative controls (e.g., heat-inactivated protein or buffer-only) to distinguish specific activity from background.

Consult the product datasheet and COA provided with each lot of rp376570 for lot-specific quality data.

Frequently asked questions

What is the recommended reconstitution buffer for Recombinant Human ASXL2 Protein?

The recommended reconstitution buffer and volume are specified in the product datasheet for rp376570. Generally, sterile ultrapure water or a physiological buffer (e.g., PBS) is suitable; however, always consult the lot-specific datasheet for exact instructions to preserve protein activity.

How should Recombinant Human ASXL2 Protein be stored to maintain stability?

Store the lyophilized protein as directed on the datasheet. After reconstitution, prepare single-use aliquots and store them at the temperature indicated in the product documentation. Avoid repeated freeze–thaw cycles, as these can reduce protein activity and lead to aggregation.

What applications is Recombinant Human ASXL2 Protein validated for?

Recombinant Human ASXL2 Protein (rp376570) is suitable for use in protein–protein interaction assays, in vitro histone modification studies, antibody validation, structural biology experiments, and as a research tool in drug discovery targeting epigenetic pathways. Please refer to the product datasheet for the full list of validated applications.

Can Recombinant Human ASXL2 Protein be used in cell-based assays?

Use in cell-based assays depends on endotoxin levels and formulation compatibility. Check the certificate of analysis (COA) for endotoxin specifications before use in cell culture systems. Consult the product datasheet for guidance on cell-based applications.

How do I verify the quality of Recombinant Human ASXL2 Protein upon receipt?

Upon receipt, inspect the vial for visible damage and confirm the lot number against the COA. Run an SDS-PAGE to verify purity and apparent molecular weight. For functional confirmation, use an activity assay appropriate to your experimental system and compare results against the specifications provided in the product datasheet.

References

1. Aladdin Scientific. Recombinant Human ASXL2 Protein, SKU rp376570. Product Datasheet (absea#PP-10118).
2. Micol J-B, Abdel-Wahab O. The Role of Additional Sex Combs-Like Proteins in Cancer. Cold Spring Harb Perspect Med. 2016.
3. Sahtoe DD, van Dijk WJ, Ekkebus R, Ovaa H, Sixma TK. BAP1/ASXL1 recruitment and activation for H2A deubiquitination. Nat Commun. 2016.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.