Recombinant Human CHCHD2P9 Protein: Use & Handling Guide

Recombinant Human CHCHD2P9 Protein is a research-grade recombinant protein intended for use in biochemical and cell biology studies investigating mitochondrial function and coiled-coil-helix-coiled-coil-helix domain biology.

Product Name

Recombinant Human CHCHD2P9 Protein

SKU

rp373245

Protein Family

CHCH domain-containing protein family

Species Origin

Human (Homo sapiens)

Application

Biochemical assays, cell biology research, functional studies

Storage Recommendation

Avoid repeated freeze-thaw cycles; aliquot upon receipt

Product Overview and Background

Recombinant Human CHCHD2P9 Protein (SKU: rp373245) is a recombinant form of the human CHCHD2P9 gene product, a member of the coiled-coil-helix-coiled-coil-helix (CHCH) domain-containing protein family. Proteins in this family are characteristically associated with mitochondrial intermembrane space localization and are implicated in mitochondrial bioenergetics and oxidative stress response pathways.

CHCHD2P9 is considered a pseudogene-derived protein variant related to CHCHD2, a gene of growing interest in neurodegeneration research, particularly in the context of Parkinson's disease and mitochondrial dysfunction. Recombinant expression of CHCHD2P9 allows researchers to probe the biochemical properties, protein-protein interactions, and potential functional roles of this protein in a controlled in vitro setting.

This product is manufactured for research use only and is not intended for diagnostic or therapeutic applications.

Reconstitution and Preparation Protocol

Before use, carefully review the Certificate of Analysis (CoA) supplied with your specific lot of rp373245 for confirmed concentration, formulation buffer composition, and recommended reconstitution volume.

1. Centrifuge the vial briefly (1,000–2,000 × g, 30 seconds) before opening to consolidate lyophilized or liquid contents at the bottom of the tube.
2. Reconstitution (if lyophilized): Add the recommended volume of sterile, ultrapure water or the buffer specified in the CoA. Do not vortex; gently pipette up and down or roll the vial between your palms to dissolve completely.
3. Allow equilibration: Let the reconstituted solution stand at room temperature for 5–10 minutes to ensure complete dissolution before use or further dilution.
4. Prepare working aliquots: Divide the reconstituted protein into single-use aliquots appropriate for your experimental needs to prevent repeated freeze-thaw cycles that can lead to protein aggregation and activity loss.
5. Carrier protein addition: If long-term stability is a concern, consider supplementing with 0.1% BSA (bovine serum albumin) as a carrier, unless this interferes with your assay format.

Note: Specific reconstitution instructions may vary by lot. Always defer to the accompanying CoA.

Storage, Stability, and Handling Precautions

Proper storage and handling are critical to maintaining the integrity and activity of Recombinant Human CHCHD2P9 Protein.

• Short-term storage (up to 1 week): Store at 4 °C if the protein will be used within one week of reconstitution. Keep on ice during bench work.
• Long-term storage: Store at −20 °C or −80 °C in single-use aliquots. −80 °C is preferred for extended storage periods.
• Freeze-thaw cycles: Minimize freeze-thaw cycles to no more than 2–3. Each cycle risks protein denaturation, aggregation, and loss of biological activity.
• Avoid contamination: Use sterile, nuclease-free pipette tips and tubes. Do not return unused protein to the original stock vial.
• Light sensitivity: Keep the vial protected from prolonged light exposure unless otherwise specified.
• General handling: Handle on ice at all times during experimental setup. Protein solutions should be kept cold to minimize proteolytic degradation.

If you observe visible precipitate, turbidity, or discoloration in the protein solution, do not use the product and contact Aladdin Scientific customer support for assistance.

Recommended Applications and Experimental Considerations

Recombinant Human CHCHD2P9 Protein is suitable for a range of in vitro research applications. Researchers should optimize conditions for their specific experimental system.

• Protein-protein interaction studies: CHCHD2P9 can be used as bait or prey in pull-down assays or co-immunoprecipitation (co-IP) experiments to identify binding partners relevant to mitochondrial pathways.
• Biochemical characterization: Suitable for SDS-PAGE, Western blot validation, and structural studies to characterize the protein's biochemical properties.
• Functional assays: Use in cell-free systems or as a supplementary reagent in cell-based assays investigating oxidative phosphorylation, mitochondrial membrane integrity, or stress response mechanisms.
• Antibody validation: Can serve as a positive control antigen for validating CHCHD2P9-specific antibodies in ELISA or Western blot formats.

Important: Optimal working concentrations, buffer conditions, and assay formats must be determined empirically by the end user for each specific application. Aladdin Scientific does not guarantee activity in every application without end-user optimization.

Quality, Safety, and Regulatory Information

Quality Control: Each lot of Recombinant Human CHCHD2P9 Protein (rp373245) is tested for purity and identity prior to release. Purity is typically assessed by SDS-PAGE and/or HPLC. Specific QC data are provided on the Certificate of Analysis (CoA) accompanying each shipment. Users are advised to verify lot-specific data before commencing experiments.

Research Use Only: This product is intended solely for research purposes. It is not approved for use in humans, animals, or clinical diagnostics.

Safety Precautions: Handle as a potential biohazard in accordance with your institution's biosafety guidelines. Wear appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection. Consult the Safety Data Sheet (SDS) for detailed hazard information before use.

Disposal: Dispose of protein solutions and contaminated materials according to applicable local, national, and institutional regulations for biological reagents.

Export Compliance: This product may be subject to export control regulations. Purchasers are responsible for ensuring compliance with all applicable laws and regulations in their jurisdiction.

Frequently asked questions

How should I reconstitute Recombinant Human CHCHD2P9 Protein (rp373245)?

Briefly centrifuge the vial before opening, then add the volume of sterile ultrapure water or recommended buffer specified on your lot's Certificate of Analysis. Gently pipette to mix—do not vortex—and allow 5–10 minutes at room temperature for complete dissolution. Refer to the CoA for lot-specific instructions.

What is the recommended storage condition for this recombinant protein?

For short-term use within one week, store at 4 °C. For long-term storage, aliquot the protein into single-use volumes and store at −20 °C or preferably −80 °C. Avoid repeated freeze-thaw cycles, which can cause aggregation and loss of activity.

What applications is Recombinant Human CHCHD2P9 Protein validated for?

This protein is suitable for use in protein-protein interaction studies (e.g., pull-down, co-IP), biochemical characterization (SDS-PAGE, Western blot), functional assays in cell-free or cell-based systems, and as a positive control antigen for antibody validation. Users should empirically optimize conditions for each specific application.

What is CHCHD2P9 and why is it relevant to research?

CHCHD2P9 is a pseudogene-derived protein related to CHCHD2, a member of the CHCH domain-containing protein family associated with mitochondrial intermembrane space function. CHCHD2 has been linked to mitochondrial bioenergetics and neurodegenerative conditions including Parkinson's disease, making CHCHD2P9 of interest for studying these pathways in vitro.

What should I do if I observe precipitation or turbidity in my protein solution?

Do not use the protein if you observe visible precipitation, turbidity, or discoloration, as these may indicate protein aggregation or degradation. Contact Aladdin Scientific customer support with your lot number and a description of the issue for technical assistance or product replacement guidance.

References

1. Aladdin Scientific Product Page: Recombinant Human CHCHD2P9 Protein, SKU rp373245.
2. Aras S, et al. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 2015.
3. Funayama M, et al. CHCHD2 mutations in autosomal dominant late-onset Parkinson's disease: a genome-wide linkage and sequencing study. Lancet Neurol. 2015.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.