Recombinant Human GABA B Receptor 2 Protein: Research Guide

Recombinant Human GABA B Receptor 2 Protein is a research-grade recombinant protein representing the second subunit of the metabotropic GABA B receptor complex, widely used in neuropharmacology, receptor-ligand interaction studies, and drug discovery programs targeting inhibitory GABAergic signaling pathways.

Product Name

Recombinant Human GABA B Receptor 2 Protein

SKU

rp367865

Target Gene / Protein

GABBR2 (GABA type B receptor subunit 2)

Protein Type

Recombinant human protein, metabotropic GPCR subunit

Primary Research Area

Neuroscience, pharmacology, GABAergic signaling

Supplier Reference

absea#PP-708

Background: GABA B Receptor 2 Biology

Gamma-aminobutyric acid type B receptors (GABA_BRs) are the principal metabotropic inhibitory receptors in the mammalian central nervous system. Unlike ionotropic GABA_A receptors, GABA_B receptors are G protein-coupled receptors (GPCRs) that modulate neuronal excitability through second-messenger cascades, inhibiting adenylyl cyclase and regulating voltage-gated calcium and potassium channels.

The functional GABA_B receptor is an obligate heterodimer composed of two subunits: GABA_BR1 (GABBR1) and GABA_BR2 (GABBR2). GABBR2 serves critical roles in receptor trafficking, G protein coupling, and signal transduction. Specifically, the GABBR2 subunit contains the transmembrane heptahelical domain responsible for G protein (G_i/o) activation, while the GABBR1 subunit harbors the orthosteric ligand-binding domain. Together, these subunits form a fully functional receptor complex essential for mediating slow inhibitory postsynaptic potentials throughout the brain and spinal cord.

GABBR2 has been implicated in numerous neurological and psychiatric conditions, including epilepsy, anxiety, depression, pain, and substance use disorders, making it a compelling target for therapeutic research and drug discovery.

Protein Characteristics and Specification

Recombinant Human GABA B Receptor 2 Protein (rp367865) is produced to research-grade standards and is designed to support a broad range of in vitro biochemical and cell-based assays. Key characteristics researchers should be aware of include:

• Target: Human GABBR2 protein (GABA type B receptor subunit 2), encoded by the GABBR2 gene.
• Format: Recombinant protein; the exact expression system, tag, and formulation details should be confirmed from the product datasheet (absea#PP-708) or by contacting Aladdin Scientific technical support.
• Purity: Manufactured to research-reagent purity specifications; specific purity data (e.g., SDS-PAGE, HPLC) are available on the certificate of analysis (CoA) supplied with each lot.
• Species: Human (Homo sapiens), ensuring relevance to human disease models and translational research.
• Application Suitability: Suitable for use in binding assays, ELISA, Western blot, and other receptor characterization studies, subject to individual optimization and validation.

Researchers are advised to consult the official product datasheet for the most current and lot-specific specifications before experimental use.

Research Applications

Recombinant Human GABA B Receptor 2 Protein supports a wide array of research applications in neuroscience and pharmacology:

• Receptor-Ligand Binding Studies: Used to characterize the binding affinity and kinetics of known GABA_B modulators (agonists, antagonists, and positive allosteric modulators) and to screen novel compounds in drug discovery pipelines.
• Protein-Protein Interaction Studies: Enables investigation of GABBR1/GABBR2 heterodimerization and identification of interacting partners involved in receptor trafficking and signaling.
• Antibody Development and Validation: Serves as an antigen for generating and validating research antibodies targeting the GABBR2 extracellular or intracellular domains.
• Structural Biology: Can be used as a component in structural studies (e.g., cryo-EM, X-ray crystallography) aiming to elucidate GABA_B receptor architecture and conformational dynamics.
• Bioassay Development: Supports development and quality control of cell-free and cell-based functional assays measuring GABA_BR-dependent G protein signaling.
• Disease Mechanism Research: Facilitates mechanistic studies in disease contexts including epilepsy, neuropathic pain, mood disorders, and addiction.

Handling, Storage, and Stability Guidelines

Proper handling and storage of Recombinant Human GABA B Receptor 2 Protein are essential to maintain activity and reproducibility across experiments. Follow these best-practice guidelines:

• Storage Temperature: Store at −20 °C or −80 °C as specified on the product datasheet and CoA. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze-thaw stress. Aliquot volumes should reflect typical experimental usage.
• Reconstitution: If supplied lyophilized, reconstitute using the buffer and volume specified on the datasheet. Allow the vial to equilibrate to room temperature before opening, and mix gently by pipetting—do not vortex.
• Working Conditions: Keep protein on ice during experimental setup. Minimize exposure to elevated temperatures and avoid prolonged incubation at room temperature.
• Contamination Prevention: Use aseptic technique when handling. Avoid introducing proteases or detergents incompatible with the formulation.
• Shelf Life: Refer to the expiration date on the vial label and the CoA. Performance is guaranteed under specified storage conditions up to the stated expiry.

For any handling questions specific to this product, contact Aladdin Scientific technical support with your lot number for tailored guidance.

Quality Assurance and Regulatory Considerations

Aladdin Scientific is committed to supplying research reagents that meet rigorous quality standards. For Recombinant Human GABA B Receptor 2 Protein (rp367865), the following quality and compliance considerations apply:

• Certificate of Analysis (CoA): Each lot is accompanied by a CoA detailing identity, purity, concentration, and functional test results. Researchers should download the lot-specific CoA from the Aladdin Scientific website or request it from customer service.
• Research Use Only (RUO): This product is intended solely for research purposes. It is not approved for diagnostic, therapeutic, or clinical use in humans or animals.
• Lot-to-Lot Consistency: While Aladdin Scientific applies consistent manufacturing controls, researchers are encouraged to validate the protein in their specific assay system upon receipt of each new lot.
• Material Safety: Consult the Safety Data Sheet (SDS) for information on hazard classification, personal protective equipment requirements, and proper disposal procedures.
• Regulatory Compliance: Import, export, and use of recombinant proteins may be subject to local biosafety and regulatory requirements. Users are responsible for ensuring compliance with applicable institutional and governmental regulations.

Frequently asked questions

What is the functional role of the GABA B Receptor 2 subunit in the receptor complex?

GABBR2 is the G protein-coupling subunit of the obligate GABBR1/GABBR2 heterodimer. It contains the transmembrane heptahelical domain that activates Gi/o proteins upon receptor stimulation, thereby mediating downstream inhibitory signaling including inhibition of adenylyl cyclase and modulation of ion channels. Without GABBR2, the receptor complex cannot couple efficiently to G proteins.

What expression system is used to produce rp367865, and does it affect functional activity?

The specific expression system for rp367865 is not detailed in the current source material; please consult the product datasheet (absea#PP-708) or contact Aladdin Scientific technical support for this information. The choice of expression system (e.g., mammalian, baculovirus, E. coli) can affect post-translational modifications and functional activity, so confirming this detail is important for activity-dependent assays.

How should I validate Recombinant Human GABA B Receptor 2 Protein in my specific assay?

Validation strategy depends on the intended application. For binding assays, confirm specific binding using known GABBR agonists or antagonists as controls. For Western blot or ELISA-based detection, use a validated anti-GABBR2 antibody and confirm the expected molecular weight band. Always include a no-protein negative control and, where feasible, a positive control with a characterized lot. Review the CoA for lot-specific quality control data as a baseline reference.

Can this protein be used for in vivo studies or clinical applications?

No. Recombinant Human GABA B Receptor 2 Protein (rp367865) is labeled Research Use Only (RUO) and is not intended or validated for in vivo administration, diagnostic use, or any clinical application. Researchers planning in vivo studies should consult regulatory guidance and use appropriately certified materials.

What are the recommended storage conditions to maintain protein stability?

Store at −20 °C or −80 °C as directed on the product datasheet and CoA. Avoid repeated freeze-thaw cycles by preparing single-use aliquots upon first thaw. Keep the protein on ice during use and do not expose it to elevated temperatures for extended periods. Refer to the CoA for lot-specific stability data and expiry information.

References

1. Bettler B, Kaupmann K, Mosbacher J, Gassmann M. Molecular structure and physiological functions of GABA(B) receptors. Physiol Rev. 2004;84(3):835-867. doi:10.1152/physrev.00036.2003
2. Ulrich D, Bettler B. GABA(B) receptors: synaptic functions and mechanisms of diversity. Curr Opin Neurobiol. 2007;17(3):298-303. doi:10.1016/j.conb.2007.04.001
3. Gassmann M, Bettler B. Regulation of neuronal GABA(B) receptor functions by subunit composition. Nat Rev Neurosci. 2012;13(6):380-394. doi:10.1038/nrn3249

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.