Recombinant Human GIPC3 Protein: Research Guide & Usage

Recombinant Human GIPC3 Protein is a research-grade recombinant protein designed to support studies in PDZ-domain-mediated signal transduction, auditory function, and GIPC family protein biology.

Product Name

Recombinant Human GIPC3 Protein

SKU

rp371068

Protein Family

GIPC (GAIP-interacting protein, C terminus) family, PDZ domain-containing proteins

Primary Research Areas

Signal transduction, auditory biology, hereditary hearing loss

Species Origin

Human (Homo sapiens)

Product Form

Recombinant protein; refer to product datasheet for formulation details

Introduction to GIPC3 and Its Biological Significance

GIPC3 (GAIP-interacting protein, C terminus, member 3) belongs to the GIPC family of scaffold proteins characterized by a central PDZ domain flanked by GH1 and GH2 homology domains. PDZ domain-containing proteins play critical roles in organizing protein complexes at the plasma membrane and mediating intracellular signaling cascades.

GIPC3 is expressed in a variety of tissues and has been implicated in receptor internalization, endosomal trafficking, and cytoskeletal organization. Notably, mutations in the GIPC3 gene have been associated with autosomal recessive non-syndromic hearing loss (DFNB15/72/95), making GIPC3 of particular interest in auditory biology and the study of hereditary deafness.

Recombinant Human GIPC3 Protein from Aladdin Scientific (SKU: rp371068) provides researchers with a reliable, research-grade tool to investigate GIPC3 function, protein–protein interactions, and its role in disease-relevant pathways.

Storage, Reconstitution, and Handling Protocol

Storage: Upon receipt, store Recombinant Human GIPC3 Protein according to the conditions specified on the product datasheet. Proteins are typically stored at −20 °C or −80 °C to maintain stability and activity. Avoid repeated freeze–thaw cycles, as this may lead to protein aggregation and loss of biological activity. Aliquot the protein into single-use volumes upon first thaw.

Reconstitution: If supplied in lyophilized form, reconstitute with the recommended volume of sterile deionized water or the buffer specified in the datasheet. Allow the vial to sit at room temperature for a few minutes after adding the reconstitution buffer, then gently mix by pipetting. Do not vortex. Centrifuge briefly if necessary to collect the contents.

Working Concentration: Optimal working concentrations depend on the specific assay. Titration experiments are recommended to determine the ideal concentration for each application. Refer to the product-specific datasheet for recommended starting concentrations.

Handling Precautions: Use pre-chilled pipette tips and tubes when handling the protein. Minimize time at room temperature. For long-term storage, adding a carrier protein such as 0.1% BSA may help stabilize the recombinant protein.

Experimental Applications

Recombinant Human GIPC3 Protein is suitable for a range of in vitro research applications. Common uses include:

• Protein–Protein Interaction Studies: GIPC3 contains a PDZ domain that mediates interactions with PDZ-binding motifs on partner proteins. Pull-down assays and co-immunoprecipitation experiments can be performed using recombinant GIPC3 to map interaction networks.
• Biochemical Assays: GIPC3 can be used as an antigen for antibody validation, as a positive control in Western blot, ELISA, or dot blot assays, and for functional binding studies.
• Structural Studies: Recombinant GIPC3 provides material for structural characterization techniques such as circular dichroism (CD) spectroscopy or size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS).
• Drug Discovery: As a disease-associated protein (hereditary hearing loss), GIPC3 may serve as a target for small-molecule or peptide inhibitor screening assays.

Always verify suitability for your specific application by consulting the product datasheet and, where necessary, conducting pilot experiments.

Quality, Purity, and Regulatory Considerations

Research Use Only: Recombinant Human GIPC3 Protein (rp371068) is intended for research use only and is not approved for diagnostic or therapeutic use in humans or animals.

Purity and Identity: Recombinant proteins from Aladdin Scientific are manufactured and quality-controlled to research-grade standards. Purity is typically assessed by SDS-PAGE and/or HPLC, while protein identity may be confirmed by mass spectrometry or N-terminal sequencing. Refer to the Certificate of Analysis (CoA) supplied with each lot for lot-specific purity and quality data.

Endotoxin Testing: For applications involving cell-based assays or sensitive biological systems, endotoxin levels should be considered. Check the product datasheet and CoA for endotoxin specifications.

Biosafety: Handle all recombinant proteins in accordance with your institution's biosafety guidelines. Follow standard laboratory good practices (GLP) when working with recombinant materials.

Frequently asked questions

What is GIPC3 and why is it relevant to hearing loss research?

GIPC3 is a PDZ domain-containing scaffold protein belonging to the GIPC family. Mutations in the GIPC3 gene have been linked to autosomal recessive non-syndromic hearing loss (DFNB15/72/95), making recombinant GIPC3 protein a valuable tool for researchers studying the molecular mechanisms of hereditary deafness and inner ear biology.

How should Recombinant Human GIPC3 Protein be stored to maintain activity?

Store the protein at −20 °C or −80 °C as directed on the product datasheet. To preserve activity, avoid repeated freeze–thaw cycles by aliquoting the protein into single-use volumes upon first thaw. Adding a carrier protein such as 0.1% BSA is recommended for long-term storage stability.

What applications is Recombinant Human GIPC3 Protein suitable for?

Recombinant Human GIPC3 Protein is suitable for protein–protein interaction studies (e.g., pull-down and co-immunoprecipitation assays), biochemical assays (Western blot, ELISA, antigen validation), structural characterization, and drug discovery screening. Always consult the product datasheet to confirm suitability for your specific application.

Is Recombinant Human GIPC3 Protein approved for clinical or diagnostic use?

No. Recombinant Human GIPC3 Protein (SKU: rp371068) is strictly for research use only and has not been evaluated or approved for use in diagnostic, therapeutic, or clinical applications.

How do I determine the optimal working concentration of GIPC3 protein for my assay?

Optimal working concentrations vary depending on the assay format and experimental conditions. It is recommended to perform a titration experiment using a range of protein concentrations to identify the dose that gives optimal signal without background interference. The product datasheet may provide suggested starting concentration ranges as a reference point.

References

1. Rehman AU, et al. Mutations in GIPC3 cause nonsyndromic hearing loss DFNB15 and DFNB95. Am J Hum Genet. 2011.
2. Aladdin Scientific. Recombinant Human GIPC3 Protein, SKU rp371068. Product Datasheet.
3. Bhatt DL, et al. GIPC family proteins and their role in PDZ-mediated signaling. Biochem J. (general textbook-level reference placeholder).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.