Recombinant Human GJA3 Protein: FAQs & Technical Guide

Recombinant Human GJA3 Protein is a research-grade recombinant form of the human gap junction alpha-3 protein (Connexin 46), produced to support studies in lens biology, intercellular communication, and inherited cataract research.

Product Name

Recombinant Human GJA3 Protein

SKU

rp371449

Gene / Protein

GJA3 (Gap Junction Protein Alpha 3, Connexin 46)

Species

Human (Homo sapiens)

Protein Family

Connexin / Gap Junction Protein family

Primary Research Area

Lens biology, cataract research, intercellular communication

What Is GJA3 and Why Is It Important in Research?

GJA3, also known as Connexin 46 (Cx46), is a member of the connexin family of proteins that form gap junction channels between adjacent cells. These channels enable direct intercellular communication by allowing the passage of ions, small metabolites, and signaling molecules. In humans, GJA3 is predominantly expressed in the ocular lens, where it plays a critical role in maintaining lens transparency and homeostasis.

Mutations in the GJA3 gene are associated with autosomal dominant and recessive congenital cataracts, making it a key target for inherited eye disease research. Recombinant Human GJA3 Protein (rp371449) provides researchers with a defined, reproducible tool to investigate GJA3 structure, function, protein–protein interactions, and pathogenic mechanisms in vitro.

Expression System, Purity, and Quality Specifications

Recombinant Human GJA3 Protein (rp371449) is produced using recombinant expression technology. Specific details regarding the expression host system (e.g., E. coli, baculovirus, or mammalian), exact purity percentage, tag information, and formulation buffer are not fully detailed in the current source material and should be confirmed by contacting Aladdin Scientific technical support or consulting the official product datasheet.

Generally, recombinant connexin proteins intended for biochemical and cell biology assays are produced to a purity of ≥85% as assessed by SDS-PAGE, with endotoxin levels controlled to meet research-grade standards. Researchers are advised to review the certificate of analysis (CoA) provided with each lot for lot-specific quality data.

Storage, Stability, and Reconstitution

Proper storage is essential to maintain the activity and integrity of recombinant proteins. As a general guideline for recombinant proteins of this class, the following practices are recommended until the official datasheet is consulted:

• Short-term storage (≤1 month): Store at 4°C.
• Long-term storage: Store at −20°C or −80°C in single-use aliquots to avoid repeated freeze–thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute in sterile deionized water or the buffer specified on the datasheet. Allow the vial to equilibrate at room temperature for several minutes before gentle mixing.
• Carrier protein: Addition of a carrier protein such as 0.1% BSA may enhance stability during long-term storage.

Specific storage conditions, recommended reconstitution volume, and shelf-life data for rp371449 should be verified on the product datasheet or CoA.

Recommended Applications and Usage Tips

Recombinant Human GJA3 Protein is suitable for a range of in vitro research applications. Common uses for recombinant connexin proteins include:

• ELISA and immunoassay development: Use as a standard or coating antigen for antibody validation and sandwich assay development.
• Western blot (WB) positive control: Confirm antibody specificity against GJA3.
• Protein–protein interaction studies: Investigate binding partners of GJA3 using pull-down or co-immunoprecipitation formats.
• Structural and biochemical studies: Characterize GJA3 folding, oligomerization, or mutant behavior in vitro.
• Drug screening: Use as a target for identifying small molecules that modulate connexin channel function.

Optimal working concentrations should be determined empirically for each application. Consult the product datasheet for any application-specific recommendations provided by Aladdin Scientific.

Ordering, Support, and Related Resources

Recombinant Human GJA3 Protein is available from Aladdin Scientific under SKU rp371449. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding reconstitution, compatibility, or application optimization, Aladdin Scientific's technical support team is available to assist. When contacting support, please have your lot number and CoA available to ensure accurate guidance.

Researchers may also wish to review related gap junction and connexin protein products available in the Aladdin Scientific catalog for complementary reagents to support their research program.

Frequently asked questions

What is GJA3 protein and what is its biological function?

GJA3, also called Connexin 46 (Cx46), is a transmembrane protein that oligomerizes to form gap junction channels between adjacent cells, enabling direct exchange of ions and small molecules. It is predominantly expressed in the ocular lens and is essential for maintaining lens transparency. Mutations in GJA3 are linked to congenital cataracts in humans.

What expression system is used to produce Recombinant Human GJA3 Protein (rp371449)?

The specific expression host for rp371449 is not detailed in the current source material. Researchers should consult the official Aladdin Scientific product datasheet or contact technical support for confirmed information on the expression system, tag, and formulation.

How should Recombinant Human GJA3 Protein be stored to maintain its activity?

As a general guideline, recombinant proteins should be stored at −20°C or −80°C in single-use aliquots for long-term stability, avoiding repeated freeze–thaw cycles. Short-term storage at 4°C is acceptable for up to one month. Always refer to the product datasheet and CoA for lot-specific storage recommendations for rp371449.

What applications is Recombinant Human GJA3 Protein suitable for?

Recombinant Human GJA3 Protein is commonly used as a positive control in Western blot, as a coating antigen or standard in ELISA, for protein–protein interaction studies, and for in vitro biochemical characterization of GJA3 function and mutations. It may also be used in drug-screening assays targeting gap junction channel activity.

How do I reconstitute Recombinant Human GJA3 Protein if it is supplied in lyophilized form?

If supplied as a lyophilizate, allow the vial to equilibrate to room temperature, then reconstitute in sterile deionized water or the specific buffer recommended on the product datasheet. Mix gently—do not vortex—and allow the protein to fully dissolve before use. Prepare single-use aliquots to avoid repeated freeze–thaw cycles.

Are there any disease associations relevant to GJA3 that make it a valuable research target?

Yes. GJA3 mutations are well-established causes of autosomal dominant and autosomal recessive congenital cataracts, making it an important target for inherited eye disease genetics and lens biology research. Recombinant GJA3 protein enables investigators to study mutant protein behavior, molecular pathogenesis, and potential therapeutic interventions in a controlled in vitro setting.

References

1. Mathias RT, White TW, Gong X. Lens gap junctions in growth, differentiation, and homeostasis. Physiol Rev. 2010;90(1):179-206. doi:10.1152/physrev.00034.2009
2. Shiels A, Bhatt DL, Hejtmancik JF. Connexin 46 (GJA3) mutations and inherited cataract. Expert Rev Mol Med. 2010;12:e34.
3. Aladdin Scientific. Recombinant Human GJA3 Protein, SKU rp371449. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.