Recombinant Human GLYATL2 Protein: Expression & Usage Protocol

Recombinant Human GLYATL2 Protein is a research-grade recombinant enzyme designed to support functional studies, activity assays, and biochemical characterization of the glycine acyltransferase-like 2 pathway in human biology.

Product Name

Recombinant Human GLYATL2 Protein

SKU

rp372889

Protein Family

Glycine acyltransferase-like (GLYATL) family

Species Origin

Human (Homo sapiens)

Application

Functional assays, enzyme activity studies, biochemical research

Storage Recommendation

Avoid repeated freeze-thaw cycles; store as directed on product datasheet

Background and Biological Context

GLYATL2 (Glycine Acyltransferase-Like 2) is a member of the glycine acyltransferase family, enzymes that catalyze the conjugation of acyl groups to glycine, playing roles in amino acid metabolism and detoxification pathways. Understanding GLYATL2 function has implications for metabolic research and the study of acyl-amino acid biochemistry in human tissues.

Recombinant Human GLYATL2 Protein (rp372889) is produced to support in vitro research applications, enabling scientists to probe enzyme kinetics, substrate specificity, and potential interaction partners in controlled experimental settings.

Materials Required

Before beginning, ensure the following materials are available:

• Recombinant Human GLYATL2 Protein (rp372889, Aladdin Scientific)
• Appropriate reconstitution buffer (refer to product datasheet for recommended buffer composition)
• Sterile, nuclease-free water or PBS (if applicable)
• Microcentrifuge tubes (low-protein-binding recommended)
• Pipettes and calibrated tips
• Ice bucket or cooling block
• Spectrophotometer or assay-specific detection equipment
• Aliquoting supplies for long-term storage

Consult the product datasheet (absea#PP-12213) for lot-specific concentration, purity, and buffer details prior to use.

Step-by-Step Protocol

Step 1 – Thawing: Remove the vial from storage and thaw on ice or at 4 °C. Do not thaw at room temperature or use a water bath at elevated temperatures, as this may reduce protein activity.

Step 2 – Reconstitution: If supplied in lyophilized form, reconstitute the protein by adding the volume of reconstitution buffer specified in the product datasheet. Gently pipette to mix; do not vortex. Allow the protein to dissolve completely before proceeding.

Step 3 – Centrifugation: Briefly centrifuge the reconstituted protein at low speed (e.g., 5,000 × g for 1 minute) to collect any residual material from the tube walls and remove aggregates.

Step 4 – Aliquoting: Divide the reconstituted protein into single-use aliquots to minimize freeze-thaw cycles. Store aliquots at −20 °C or −80 °C as recommended on the datasheet.

Step 5 – Quality Check: Verify protein integrity by SDS-PAGE or the assay method specified in the product documentation prior to use in downstream applications.

Step 6 – Application Setup: Use the protein at concentrations optimized for your specific assay. Refer to published literature on GLYATL2 enzyme assays for guidance on substrate concentrations, reaction buffers, and incubation conditions.

Storage, Stability, and Handling Precautions

Storage: Store lyophilized or reconstituted Recombinant Human GLYATL2 Protein under conditions specified in the product datasheet. Generally, lyophilized proteins are stable at −20 °C for extended periods, while reconstituted protein should be aliquoted and stored at −80 °C for long-term use.

Stability: Avoid repeated freeze-thaw cycles, which can cause protein denaturation and loss of activity. Adding a carrier protein such as BSA (0.1% final concentration) to the reconstitution buffer may help stabilize dilute protein solutions.

Handling: Always work on ice when handling the reconstituted protein. Use low-protein-binding tubes and tips to minimize adsorptive losses. Maintain aseptic technique if the protein is to be used in cell-based assays.

Safety: Handle according to standard laboratory safety practices for recombinant proteins. Refer to the Safety Data Sheet (SDS) and product documentation for specific hazard information.

Troubleshooting

Problem: Low or no enzyme activity detected.
Possible causes: Protein degradation due to improper thawing; excessive freeze-thaw cycles; incorrect assay buffer conditions. Recommended actions: Thaw gently on ice; use fresh aliquots; verify assay buffer pH and ionic strength against published GLYATL2 assay conditions.

Problem: Protein aggregation or precipitation observed.
Possible causes: Reconstitution at too high a concentration; incompatible buffer; temperature shock during thawing. Recommended actions: Reconstitute at recommended concentration; centrifuge to remove aggregates; ensure gradual thawing on ice.

Problem: Inconsistent results between experiments.
Possible causes: Variability in protein concentration between aliquots; pipetting errors; reagent lot differences. Recommended actions: Measure protein concentration before each experiment using a standard assay (e.g., BCA or Bradford); prepare fresh standards; contact Aladdin Scientific technical support for lot-specific guidance.

Frequently asked questions

What is the recommended reconstitution buffer for Recombinant Human GLYATL2 Protein?

The recommended reconstitution buffer and volume are specified in the product datasheet (absea#PP-12213). Typically, sterile PBS or a mild physiological buffer is used. Always consult the lot-specific datasheet before reconstitution to ensure optimal activity.

How should Recombinant Human GLYATL2 Protein be stored to maintain activity?

Lyophilized protein should be stored at −20 °C. After reconstitution, divide into single-use aliquots and store at −80 °C for long-term use. Avoid repeated freeze-thaw cycles, as these can lead to protein degradation and loss of enzymatic activity.

What applications is Recombinant Human GLYATL2 Protein suitable for?

Recombinant Human GLYATL2 Protein (rp372889) is intended for research applications including enzyme activity and kinetics studies, substrate specificity assays, protein interaction studies, and biochemical characterization of the GLYATL2 enzyme. It is for research use only and not intended for diagnostic or therapeutic use.

How can I verify the quality and integrity of the protein upon receipt?

Upon receipt, inspect the vial for any signs of damage or leakage. After reconstitution, protein integrity can be assessed by SDS-PAGE to confirm molecular weight and purity. Functional activity may be confirmed using an appropriate enzyme activity assay as described in published literature for GLYATL2.

References

1. Aladdin Scientific. Recombinant Human GLYATL2 Protein, SKU rp372889. Product Datasheet absea#PP-12213.
2. UniProt Consortium. GLYATL2 – Glycine acyltransferase-like protein 2 – Homo sapiens (Human). UniProtKB entry. [Verification required for specific accession number and details.]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.