Recombinant Human GOLGA6L6 Protein: Key FAQs

Recombinant Human GOLGA6L6 Protein is a research-grade recombinant protein derived from the human GOLGA6L6 gene, a member of the Golgin family of coiled-coil proteins associated with Golgi apparatus structure and function.

Product Name

Recombinant Human GOLGA6L6 Protein

SKU

rp377477

Protein Family

Golgin family (coiled-coil Golgi-associated proteins)

Species Origin

Human (Homo sapiens)

Application

In vitro research, biochemical assays, antibody development

Storage Recommendation

Follow supplier-specified storage conditions; avoid repeated freeze-thaw cycles

What Is GOLGA6L6 and Why Is It Relevant to Research?

GOLGA6L6 (Golgin A6 Family Member L6) is a member of the Golgin superfamily, a group of coiled-coil proteins predominantly localized to the Golgi apparatus. Golgins play critical structural roles in maintaining Golgi morphology, vesicle tethering, and membrane trafficking. GOLGA6L6 is encoded by a gene that has undergone segmental duplication and is part of an expanded gene family in primates.

Research interest in GOLGA6L6 and related Golgin family members has grown due to their potential roles in intracellular trafficking pathways and their implication in certain disease contexts. Recombinant human GOLGA6L6 protein provides a defined, reproducible reagent for studying protein–protein interactions, developing specific antibodies, and investigating Golgi-associated biology in vitro.

How Is Recombinant Human GOLGA6L6 Protein Produced and What Is Its Purity?

Recombinant Human GOLGA6L6 Protein (SKU: rp377477) is produced using standard recombinant protein expression technologies. Typical production platforms for recombinant human proteins include Escherichia coli, baculovirus-insect cell systems, or mammalian expression systems, each offering different post-translational modification profiles.

Purity is generally assessed by SDS-PAGE and may be further confirmed by Western blot or mass spectrometry. For specific purity values, expression host details, tag information, and endotoxin levels for rp377477, researchers should consult the official product datasheet available from Aladdin Scientific or contact technical support, as these parameters are product-lot-specific and not reproduced here to avoid inaccuracy.

How Should Recombinant Human GOLGA6L6 Protein Be Stored and Reconstituted?

Proper storage and reconstitution are critical to maintaining the biological activity and integrity of recombinant proteins. As a general best practice for recombinant proteins:

• Storage: Lyophilized proteins are typically stored at −20 °C or −80 °C in a desiccated environment. Liquid formulations are generally stored at −80 °C. Always refer to the specific product datasheet for rp377477 for confirmed storage conditions.
• Reconstitution: Lyophilized protein should be reconstituted with the buffer recommended on the datasheet (commonly sterile water or PBS), allowed to fully dissolve, and then aliquoted to avoid repeated freeze-thaw cycles.
• Freeze-Thaw: Repeated freeze-thaw cycles should be avoided as they can cause protein aggregation and loss of activity. Single-use aliquots are strongly recommended.

If carrier protein (e.g., BSA) is required for stability during long-term storage, this will be indicated on the product-specific datasheet. Contact Aladdin Scientific technical support for guidance specific to this product.

What Are the Recommended Research Applications for GOLGA6L6 Recombinant Protein?

Recombinant Human GOLGA6L6 Protein is suitable for a range of in vitro research applications common to recombinant Golgin family proteins, including but not limited to:

• Antibody Production and Validation: Use as an immunogen for raising polyclonal or monoclonal antibodies, or as a positive control antigen in ELISA and Western blot assays.
• Protein–Protein Interaction Studies: Investigate binding partners and interaction networks of GOLGA6L6 using pull-down assays, co-immunoprecipitation, or surface plasmon resonance.
• Functional Assays: Study the role of GOLGA6L6 in Golgi trafficking or structural maintenance using cell-free biochemical systems.
• Structural Studies: Use as a reference standard or for structural biology investigations.

Verified application-specific data for rp377477 should be confirmed via the product datasheet or by contacting Aladdin Scientific technical support before experimental design.

How to Order Recombinant Human GOLGA6L6 Protein from Aladdin Scientific

Recombinant Human GOLGA6L6 Protein is available from Aladdin Scientific under SKU rp377477. To place an order:

• Search for SKU rp377477 on the Aladdin Scientific website or contact your regional sales representative.
• Review the product datasheet for current lot-specific specifications including purity, formulation, and concentration prior to ordering.
• For bulk quantities, custom formulations, or project-specific requirements, contact Aladdin Scientific customer service or technical support directly.
• Ensure compliance with your institution's procurement and biosafety policies when ordering recombinant proteins.

For questions about pricing, availability, shipping conditions, or returns, refer to Aladdin Scientific's standard ordering, shipping, and returns policies available on the company website.

Frequently asked questions

What is GOLGA6L6 and what biological role does it serve?

GOLGA6L6 is a member of the Golgin A6 protein family, a group of coiled-coil proteins associated with the Golgi apparatus. Golgins are important for maintaining Golgi structure, mediating vesicle tethering, and supporting intracellular membrane trafficking. GOLGA6L6 is part of a primate-specific expanded gene family resulting from segmental duplication events.

What expression system is used to produce Recombinant Human GOLGA6L6 Protein (rp377477)?

The specific expression system, tag configuration, and formulation details for rp377477 are provided in the product-specific datasheet available from Aladdin Scientific. Common expression systems for recombinant human proteins include E. coli, baculovirus-insect cell, and mammalian systems. Please download the current datasheet or contact technical support for confirmed specifications.

How should I reconstitute and store Recombinant Human GOLGA6L6 Protein to maintain its activity?

Lyophilized recombinant proteins should be reconstituted using the buffer specified in the product datasheet, typically sterile water or PBS, and allowed to dissolve completely before aliquoting. Store aliquots at −80 °C and avoid repeated freeze-thaw cycles, which can lead to aggregation and reduced activity. Always refer to the rp377477 datasheet for product-specific storage and handling instructions.

Is Recombinant Human GOLGA6L6 Protein (rp377477) suitable for use as an antibody immunogen?

Recombinant proteins are widely used as immunogens for raising antibodies against specific targets, and GOLGA6L6 recombinant protein is generally suitable for this application. However, suitability depends on factors such as the protein's folding state, presence of a purification tag, and the target epitopes of interest. Researchers should review the datasheet and consult Aladdin Scientific technical support to confirm suitability for immunization protocols.

Can I use Recombinant Human GOLGA6L6 Protein in SDS-PAGE or Western blot as a positive control?

Yes, recombinant proteins such as rp377477 are commonly used as positive controls in SDS-PAGE and Western blot experiments to confirm antibody specificity and assay performance. Ensure you use an amount within the linear detection range of your assay. Check the datasheet for the expected molecular weight under reducing and non-reducing conditions.

References

1. Barr FA, Nakamura N, Warren G. Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae. EMBO J. 1998. [General Golgin family reference — not specific to GOLGA6L6; included for contextual background only]
2. Aladdin Scientific. Recombinant Human GOLGA6L6 Protein, SKU rp377477. Product Datasheet. [Accessed via Aladdin Scientific website]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.