Recombinant Human GOLGA8H Protein: Properties & Applications

Recombinant Human GOLGA8H Protein is a research-grade recombinant protein derived from the human GOLGA8H gene, designed to support studies of Golgi apparatus structure, membrane trafficking, and associated cellular functions.

Product Name

Recombinant Human GOLGA8H Protein

SKU

rp377488

Gene

GOLGA8H (Golgin subfamily A member 8H)

Species

Human (Homo sapiens)

Protein Family

Golgin / Golgi autoantigen family

Research Area

Golgi biology, intracellular membrane trafficking, cell biology

Background: The GOLGA8H Gene and Protein

GOLGA8H (Golgi Autoantigen, Golgin Subfamily A, Member 8H) is a member of the golgin family of coiled-coil proteins that are predominantly localized to the Golgi apparatus. Golgins serve as structural scaffolds that maintain Golgi ribbon architecture and facilitate vesicle tethering and membrane trafficking between Golgi cisternae. The GOLGA8H gene is part of a larger cluster of paralogous golgin-A8 family members found on human chromosome 15, a region known for genomic instability and copy number variation. While the precise molecular functions of GOLGA8H remain under active investigation, family members share conserved coiled-coil domains implicated in protein-protein interactions at the Golgi membrane.

Research into golgin proteins, including GOLGA8H, has implications for understanding organelle biogenesis, secretory pathway regulation, and diseases associated with Golgi dysfunction.

Product Description and Recombinant Protein Specifications

Aladdin Scientific's Recombinant Human GOLGA8H Protein (SKU: rp377488) is produced using recombinant expression technology to deliver a high-quality protein suitable for a range of in vitro research applications. Recombinant proteins in this category are typically expressed in bacterial, insect, or mammalian cell systems, with purification strategies tailored to yield a soluble, functional product.

Researchers should consult the product datasheet for confirmed details on host expression system, tag configuration, molecular weight, purity grade, formulation buffer, and recommended storage conditions. All specification data provided on the datasheet supersedes general descriptions and should be used as the primary reference for experimental planning.

Note: For precise specifications including purity percentage, endotoxin levels, and activity data, please refer to the official product page or contact Aladdin Scientific technical support.

Research Applications

Recombinant Human GOLGA8H Protein is intended for research use only and may be applicable in the following contexts:

• Biochemical assays: Use as an antigen for antibody validation, ELISA standard, or Western blot positive control.
• Protein interaction studies: Investigate binding partners of GOLGA8H within the Golgi proteome using pull-down or co-immunoprecipitation approaches.
• Structural studies: Contribute to structural characterization of golgin coiled-coil domains.
• Cell biology research: Support functional studies of Golgi organization and intracellular trafficking pathways.
• Genomic instability research: Given the chromosomal 15q context of GOLGA8H, the protein may be relevant to studies of copy number variation and segmental duplication biology.

This product is not intended for diagnostic, therapeutic, or clinical use.

Handling, Storage, and Quality Considerations

To maintain protein integrity and ensure reproducible results, researchers should observe the following general best practices when working with Recombinant Human GOLGA8H Protein:

• Upon receipt, briefly centrifuge the vial before opening to collect the contents at the bottom.
• Reconstitute lyophilized protein (if applicable) according to the datasheet instructions using the recommended buffer.
• Aliquot the protein into single-use volumes to avoid repeated freeze-thaw cycles, which can degrade activity and increase aggregation.
• Store at the temperature specified on the product datasheet; typically –20°C or –80°C for long-term storage.
• Avoid prolonged exposure to room temperature or repeated freeze-thaw cycles.

For specific storage buffer composition, stability data, and reconstitution volumes, always refer to the current product datasheet available on the Aladdin Scientific product page.

Frequently asked questions

What is GOLGA8H and why is it relevant to cell biology research?

GOLGA8H is a member of the golgin family of coiled-coil proteins localized to the Golgi apparatus. Golgins play essential roles in maintaining Golgi structure and facilitating intracellular membrane trafficking. Research on GOLGA8H contributes to understanding Golgi biogenesis, secretory pathway regulation, and the consequences of chromosomal instability in the 15q region.

What applications is Recombinant Human GOLGA8H Protein (rp377488) suitable for?

This recombinant protein is suitable for research applications including antibody validation, ELISA standards, Western blot positive controls, protein-protein interaction studies, and structural or functional characterization of Golgi-associated proteins. It is intended for research use only and not for diagnostic or therapeutic purposes.

How should Recombinant Human GOLGA8H Protein be stored to maintain stability?

The protein should be stored according to the conditions specified on the product datasheet, generally at –20°C or –80°C for long-term storage. To preserve activity, aliquot the protein into single-use volumes upon receipt and avoid repeated freeze-thaw cycles. Always consult the current datasheet for buffer composition and specific stability recommendations.

Where can I find detailed specifications such as purity, molecular weight, and endotoxin levels for rp377488?

Detailed specifications including purity, molecular weight, expression host, tag information, endotoxin levels, and formulation details are provided on the official product datasheet available on the Aladdin Scientific website. For further technical assistance, contact Aladdin Scientific's technical support team directly.

References

1. Gillingham AK, Munro S. The small G proteins of the Arf family and their regulators. Annual Review of Cell and Developmental Biology. 2007;23:579-611. [General golgin/Golgi biology reference — verify applicability to GOLGA8H specifically]
2. Barr FA, Short B. Golgins in the structure and dynamics of the Golgi apparatus. Current Opinion in Cell Biology. 2003;15(4):405-413. [General golgin structural biology — verify relevance to GOLGA8H]
3. Sudmant PH et al. Diversity of human copy number variation and multicopy genes. Science. 2010;330(6004):641-646. [Relevant to 15q copy number variation context of GOLGA8H gene cluster — verify specificity]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.