Recombinant Human KYAT1 Protein: FAQs & Technical Guide

Recombinant Human KYAT1 Protein is a research-grade recombinant enzyme representing human kynurenine aminotransferase I, an essential pyridoxal-5′-phosphate-dependent transaminase involved in the kynurenine pathway and the biosynthesis of kynurenic acid.

Product Name

Recombinant Human KYAT1 Protein

SKU

rp368909

Protein Function

Kynurenine aminotransferase I; catalyzes transamination of kynurenine to kynurenic acid using pyridoxal-5′-phosphate as cofactor

UniProt Gene Name

KYAT1 (also known as CCBL1, KATI)

Species

Human (Homo sapiens)

Application

Biochemical assays, enzyme activity studies, kynurenine pathway research, drug target investigation

What Is KYAT1 and Why Is It Important in Research?

KYAT1 (Kynurenine Aminotransferase I), also referred to as CCBL1 or KATI, is a pyridoxal-5′-phosphate (PLP)-dependent enzyme that catalyzes the irreversible transamination of L-kynurenine to kynurenic acid (KYNA). This reaction is a critical branch point of the kynurenine pathway, which is a major route for tryptophan catabolism in mammals.

Kynurenic acid is an endogenous antagonist of ionotropic glutamate receptors and the alpha-7 nicotinic acetylcholine receptor, making KYAT1 a subject of intense investigation in neurological and psychiatric disease research, including schizophrenia, Alzheimer's disease, and depression. Beyond the central nervous system, KYAT1 is also studied in the context of immune modulation, cancer biology, and metabolic disease, where the kynurenine pathway plays regulatory roles.

Recombinant Human KYAT1 Protein enables researchers to study enzyme kinetics, screen inhibitors, validate assay conditions, and interrogate the biochemical consequences of KYAT1 modulation in a controlled in vitro system.

Technical Specifications and Quality of rp368909

Aladdin Scientific's Recombinant Human KYAT1 Protein (rp368909) is produced as a recombinant protein for use in research applications. The following technical points are relevant for experimental planning:

• Gene: KYAT1 (Human)
• Expression Host: Specific expression system details (e.g., E. coli, baculovirus/insect cell, or mammalian) should be confirmed from the product datasheet or by contacting Aladdin Scientific technical support.
• Tag: Tag configuration (His-tag, GST-tag, or tag-free) should be verified on the current product datasheet, as this affects downstream assay compatibility.
• Purity: Purity specification should be confirmed from the current lot-specific Certificate of Analysis (CoA). Research-grade recombinant proteins typically target ≥85–95% purity by SDS-PAGE, but the CoA is the authoritative source.
• Formulation & Storage: Supplied lyophilized or in liquid format as specified on the datasheet. Recommended storage is typically at −20°C or −80°C; avoid repeated freeze-thaw cycles. Specific buffer composition should be confirmed from the datasheet.

Researchers are strongly encouraged to download the current product datasheet and lot-specific CoA from the Aladdin Scientific website or request them through customer support before experimental use.

Recommended Applications and Handling Best Practices

Recombinant Human KYAT1 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Enzyme Activity Assays: Measuring transamination of kynurenine to kynurenic acid under defined conditions with PLP as cofactor.
• Inhibitor Screening: Testing small-molecule compounds for their ability to inhibit KYAT1 enzymatic activity, relevant for CNS drug discovery programs.
• Biochemical Characterization: Studying substrate specificity, kinetic parameters (Km, Vmax), and cofactor requirements.
• Pathway Research: Investigating the role of KYAT1 in the kynurenine pathway in contexts such as neuroinflammation, immunology, and cancer metabolism.
• Protein–Protein Interaction Studies: As a binding partner in pull-down or co-immunoprecipitation experiments (tag-dependent; verify tag configuration).

Handling Best Practices: Reconstitute lyophilized protein according to datasheet instructions, typically using sterile water or the provided buffer. Prepare single-use aliquots to minimize freeze-thaw cycles. Include PLP in activity assay buffers, as KYAT1 is PLP-dependent. Centrifuge briefly after thawing to collect contents. Keep protein on ice during use. Avoid vortexing, which may cause denaturation.

Ordering, Storage, and Customer Support

How to Order: Recombinant Human KYAT1 Protein (SKU: rp368909) can be ordered directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative. Bulk quantities and custom formulations may be available upon request.

Storage Conditions: Upon receipt, store the product according to the conditions specified on the product label and datasheet. Typically, recombinant proteins are stored at −20°C for short-term use or −80°C for long-term storage. Prepare aliquots to prevent repeated freeze-thaw cycles, which can reduce protein activity and stability.

Shipping: Products are shipped under cold-chain conditions appropriate to the formulation. Upon receipt, inspect the package and product vial for integrity, and record the lot number for traceability.

Technical Support: Aladdin Scientific's technical support team can assist with questions on protein reconstitution, assay setup, storage troubleshooting, and compatibility with downstream applications. Contact us through the website or by email/phone as listed on the product page.

Returns and Refunds: Aladdin Scientific's standard return and refund policy applies. Please contact customer service within the specified window if any product quality concerns arise upon receipt.

Frequently asked questions

What is the biological function of KYAT1?

KYAT1 (Kynurenine Aminotransferase I) is a pyridoxal-5′-phosphate (PLP)-dependent transaminase that catalyzes the transamination of L-kynurenine to kynurenic acid, a neuroactive metabolite and endogenous antagonist of NMDA-type glutamate receptors and alpha-7 nicotinic acetylcholine receptors. KYAT1 is an important enzyme in the kynurenine pathway of tryptophan metabolism and is studied in the context of neurological diseases, immune regulation, and cancer biology.

Does KYAT1 require a cofactor for enzymatic activity?

Yes. KYAT1 is a PLP (pyridoxal-5′-phosphate)-dependent enzyme, meaning PLP is an essential cofactor for its transaminase activity. When setting up in vitro enzyme activity assays with Recombinant Human KYAT1 Protein, researchers should ensure that PLP is included in the assay buffer at an appropriate concentration. Failure to supplement PLP may result in reduced or absent enzymatic activity.

What are the recommended storage conditions for Recombinant Human KYAT1 Protein (rp368909)?

Upon receipt, store Recombinant Human KYAT1 Protein according to the conditions specified on the product label and datasheet. Generally, recombinant proteins are stored at −20°C for short-term use or −80°C for long-term storage. To maintain protein integrity and activity, prepare single-use aliquots and avoid repeated freeze-thaw cycles. For exact buffer composition and reconstitution instructions, consult the current lot-specific Certificate of Analysis (CoA) and product datasheet available from Aladdin Scientific.

What research applications is Recombinant Human KYAT1 Protein suitable for?

Recombinant Human KYAT1 Protein (rp368909) is suitable for in vitro research applications including enzyme activity assays, inhibitor screening for drug discovery, kinetic characterization (Km, Vmax), kynurenine pathway studies, and protein interaction studies. It is widely used in neuroscience, immunology, oncology, and metabolic disease research. Suitability for specific assay formats should be verified with the product datasheet or Aladdin Scientific technical support.

How can I obtain the Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for rp368909?

The Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for Recombinant Human KYAT1 Protein (rp368909) can be downloaded directly from the product page on the Aladdin Scientific website by entering the lot number. If you require assistance locating these documents, please contact Aladdin Scientific customer support with your order details and lot number.

References

1. Han Q, Robinson H, Cai T, Tagle DA, Li J. Biochemical and structural properties of mouse kynurenine aminotransferase III. Mol Cell Biol. 2009;29(3):784-793.
2. Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012;13(7):465-477.
3. Aladdin Scientific. Recombinant Human KYAT1 Protein (rp368909) Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.