Recombinant Human LANCL1 Protein: Biology & Research Use

Recombinant Human LANCL1 Protein is a prokaryotic or eukaryotically expressed form of the human LanC-like protein 1, a peripheral membrane-associated GTPase-related factor implicated in cellular energy sensing, signal transduction, and metabolic regulation.

Product Name

Recombinant Human LANCL1 Protein

SKU

rp369007

Protein Family

LanC-like (LANCL) protein family, homologous to bacterial LanC enzymes

Species

Human (Homo sapiens)

Primary Research Areas

Signal transduction, metabolic regulation, energy sensing, neuroscience

Form

Recombinant protein (expression system details: see product datasheet)

What Is LANCL1? Gene, Structure, and Protein Family

LANCL1 (LanC-like protein 1) is a member of the LanC-like protein family, which shares structural homology with bacterial LanC enzymes involved in lantibiotic biosynthesis. In mammals, LANCL1 functions as a peripheral membrane-associated protein and has been studied in the context of GTPase-related activity and nucleotide binding.

The human LANCL1 gene encodes a protein of approximately 45 kDa. LANCL1 is broadly expressed across human tissues, with notably high expression in the brain, making it of particular interest in neuroscience and metabolic research. The protein localizes primarily to the plasma membrane and cytoplasm, where it is thought to participate in signal transduction cascades.

The LANCL family in humans includes LANCL1, LANCL2, and LANCL3. While LANCL2 has been more extensively characterized as a receptor for abscisic acid (ABA) and a regulator of immune and metabolic responses, LANCL1 is increasingly recognized for its roles in neuronal signaling and energy homeostasis.

Biochemical Properties and Production of Recombinant LANCL1

Recombinant Human LANCL1 Protein (rp369007) is produced using recombinant DNA technology to ensure consistent quality, batch-to-batch reproducibility, and suitability for a range of in vitro assays. Recombinant production allows researchers to obtain the protein in quantities and purity levels not readily achievable from natural sources.

Key biochemical properties associated with LANCL1 include its ability to bind GTP and ATP, consistent with its classification as a GTPase-related protein. The protein's peripheral membrane association is mediated in part through palmitoylation, a post-translational lipid modification. When produced recombinantly, the lipid modification status may differ from the native protein; researchers should consult the product datasheet for specific information regarding post-translational modifications, tag design, purity grade, and storage conditions for SKU rp369007.

Purity of recombinant proteins from Aladdin Scientific is assessed by SDS-PAGE and/or HPLC. Endotoxin levels are controlled and tested to ensure suitability for cell-based assays. For precise specifications—including molecular weight of the recombinant construct, expression host, buffer composition, and concentration—please refer to the official product datasheet for rp369007.

Research Applications of Recombinant Human LANCL1 Protein

Recombinant Human LANCL1 Protein is a versatile reagent suitable for multiple research applications. Common uses include:

• In vitro binding and interaction assays: LANCL1 can be used as bait or prey protein in pull-down assays to identify novel protein–protein interactions within signaling networks.
• Enzyme activity assays: As a GTPase-related protein, LANCL1 is used to study nucleotide binding and hydrolysis activity under defined in vitro conditions.
• Antibody validation: Recombinant LANCL1 serves as a positive control or immunogen for validating anti-LANCL1 antibodies in western blot, ELISA, and immunoprecipitation applications.
• Structural studies: Purified recombinant protein provides the starting material for X-ray crystallography, cryo-EM, or NMR-based structural investigations.
• Cell-based functional studies: When used in cell treatment or transfection-based experiments, recombinant LANCL1 helps elucidate the protein's role in energy sensing and neuronal metabolism.

Researchers in neuroscience, metabolic biology, and chemical biology will find recombinant LANCL1 particularly relevant given the protein's emerging roles in brain glucose metabolism and potential links to neurological conditions.

Handling, Storage, and Quality Considerations

To maintain the activity and integrity of Recombinant Human LANCL1 Protein, researchers should adhere to the following general best practices, while always consulting the specific product datasheet for rp369007:

• Storage: Recombinant proteins are generally stored at −80 °C for long-term stability. Avoid repeated freeze-thaw cycles by preparing single-use aliquots upon receipt.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified in the datasheet. Allow the protein to equilibrate to the appropriate temperature before use.
• Handling: Use low-binding microtubes and pipette tips to minimize protein adsorption to surfaces. Keep the protein on ice during experimental setup.
• Quality verification: Confirm protein integrity by running an SDS-PAGE gel before critical experiments. Check for aggregation by dynamic light scattering (DLS) if structural activity is critical.
• Carrier proteins: For experiments requiring very low protein concentrations, consider adding BSA or another carrier to prevent loss through adsorption.

Aladdin Scientific performs rigorous QC testing on all recombinant proteins, including purity analysis and functional testing where applicable. Certificate of Analysis (CoA) documents are available for each lot upon request.

Frequently asked questions

What is LANCL1 and why is it relevant to my research?

LANCL1 (LanC-like protein 1) is a mammalian peripheral membrane protein homologous to bacterial LanC enzymes. It is implicated in GTP/ATP binding, energy sensing, neuronal metabolism, and signal transduction. Researchers in neuroscience, metabolic biology, and protein biochemistry will find it particularly relevant due to its high expression in the brain and its emerging roles in glucose metabolism and cellular signaling.

What applications is Recombinant Human LANCL1 Protein (rp369007) suitable for?

Recombinant Human LANCL1 Protein is suitable for in vitro binding assays, enzyme activity studies, antibody validation (western blot, ELISA, immunoprecipitation), structural biology studies, and cell-based functional assays. Please refer to the product datasheet for a confirmed application list specific to lot rp369007.

How should I store and handle Recombinant Human LANCL1 Protein?

For long-term storage, keep the protein at −80 °C and avoid repeated freeze-thaw cycles by aliquoting into single-use portions. During experiments, keep the protein on ice and use low-binding tubes and tips. Always refer to the specific storage and reconstitution instructions on the rp369007 product datasheet, as these take precedence over general guidelines.

How does Recombinant Human LANCL1 differ from native LANCL1 protein?

Recombinant LANCL1 is produced in a heterologous expression system (e.g., E. coli, insect cells, or mammalian cells) and may carry affinity tags to facilitate purification. Post-translational modifications such as palmitoylation, which influence native LANCL1 membrane association, may be absent or altered depending on the expression host. Consult the rp369007 datasheet for details on the specific construct and expression system used.

References

1. Sturla L, et al. LANCL2 is necessary for abscisic acid binding and signaling in human granulocytes and in rat insulinoma cells. J Biol Chem. 2009;284(41):28045-28057. doi:10.1074/jbc.M109.035329
2. Aladdin Scientific. Recombinant Human LANCL1 Protein (rp369007) Product Datasheet. Aladdin Scientific; [current version].
3. Mayer M, et al. LanC proteins are widely distributed in the tree of life and are characterised by different GTP- and ATP-binding and hydrolysis motifs. PeerJ. 2019;7:e6659. doi:10.7717/peerj.6659

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.