Recombinant Human MED12L Protein: FAQs & Technical Guide

Recombinant Human MED12L Protein is a research-grade recombinant protein representing the mediator complex subunit 12-like protein, essential for studying transcriptional regulation and mediator complex biology in human cells.

Product Name

Recombinant Human MED12L Protein

SKU

rp368224

Protein Target

MED12L (Mediator Complex Subunit 12-Like)

Species

Human (Homo sapiens)

Application

Research use only; suitable for biochemical and cell-biology studies

Supplier Reference

absea#PP-3243

What Is MED12L and Why Is It Important?

MED12L (Mediator Complex Subunit 12-Like) is a paralog of MED12 and functions as a structural and regulatory subunit of the Mediator complex, a large multi-protein assembly that acts as a transcriptional coactivator in eukaryotic cells. The Mediator complex bridges gene-specific transcription factors and the general RNA polymerase II machinery, thereby enabling precise control of gene expression programs.

MED12L is implicated in developmental gene regulation and has been studied in the context of neurodevelopmental disorders and cancer biology. Its paralogy with MED12 suggests overlapping yet distinct functional roles, making recombinant MED12L protein a valuable tool for dissecting Mediator complex assembly, protein–protein interactions, and transcriptional regulation studies.

Product Specifications and Quality

Recombinant Human MED12L Protein (rp368224) is produced using recombinant expression technology to yield a protein suitable for research applications. The product is referenced under supplier code absea#PP-3243.

Key specification considerations include:

• Purity: Recombinant proteins in this category are typically assessed by SDS-PAGE and may be further verified by Western blot. Confirm lot-specific purity data on the certificate of analysis (CoA).
• Formulation: The buffer composition, protein concentration, and any carrier proteins or stabilizers should be verified in the product datasheet or CoA prior to use.
• Endotoxin levels: For cell-based assays, check the CoA for endotoxin testing results.

Review flag: Specific purity percentage, host expression system, molecular weight confirmation, and exact buffer formulation were not available in the source material and should be confirmed in the product datasheet.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are critical to maintaining activity and integrity throughout the product's shelf life. The following general best-practice guidelines apply to Recombinant Human MED12L Protein:

• Short-term storage: Store at 4 °C if the product will be used within 1–2 weeks of receipt.
• Long-term storage: Aliquot the protein into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Avoid repeated freeze-thaw cycles: Each freeze-thaw event can degrade protein quality; prepare working aliquots upon first thaw.
• Carrier protein addition: If the protein is supplied without a carrier such as BSA, consider adding a stabilizing carrier per the datasheet recommendation.

Review flag: Exact recommended storage temperature, shelf life, and reconstitution instructions should be verified against the official product datasheet and CoA for lot rp368224.

Recommended Research Applications

Recombinant Human MED12L Protein is intended for research use and may be applicable across a range of biochemical and cell biology experiments. Typical applications for recombinant mediator subunit proteins include:

• Protein–protein interaction studies: Co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) to map MED12L interactions within the Mediator complex or with transcription factors.
• Structural biology: Use as a component in reconstitution experiments to study Mediator sub-module architecture.
• Antibody validation: Positive control antigen for validating anti-MED12L antibodies by Western blot or ELISA.
• Biochemical assays: Enzyme-linked assays or activity studies related to transcriptional coactivation pathways.
• Drug discovery: Target protein for screening small-molecule inhibitors of Mediator complex assembly or MED12L-related protein interactions.

Review flag: Specific validated applications with supporting experimental data were not available in the source material; confirm validated applications in the official datasheet.

Ordering, Documentation, and Support

Recombinant Human MED12L Protein is available from Aladdin Scientific under SKU rp368224 (supplier reference absea#PP-3243). To ensure a smooth procurement and research workflow, consider the following:

• Certificate of Analysis (CoA): Request the lot-specific CoA at the time of order to confirm purity, concentration, and quality control testing results.
• Safety Data Sheet (SDS): Available upon request; review prior to handling in accordance with your institution's safety protocols.
• Technical support: Contact Aladdin Scientific's technical support team for application-specific guidance, custom formulation inquiries, or troubleshooting assistance.
• Returns and refunds: Consult Aladdin Scientific's returns and refund policy if the product does not meet stated specifications upon receipt.
• Bulk and custom orders: Inquire directly with Aladdin Scientific for large-quantity pricing or custom protein formulations.

Frequently asked questions

What is the biological function of MED12L?

MED12L encodes a subunit of the Mediator complex, a conserved multiprotein coactivator that facilitates communication between transcription factors and RNA polymerase II during gene transcription. As a paralog of MED12, MED12L is believed to participate in Mediator complex assembly and transcriptional regulation, with potential roles in development and disease. Specific functional details for MED12L should be verified against current primary literature.

In which expression system is rp368224 produced?

The specific expression system (e.g., E. coli, insect cells, mammalian cells, or wheat germ) used for Recombinant Human MED12L Protein (rp368224) was not specified in the available source material. We recommend consulting the official product datasheet or contacting Aladdin Scientific technical support for confirmed expression system details, as this may affect post-translational modification status and suitability for certain assays.

How should Recombinant Human MED12L Protein be stored to maintain stability?

As a general best practice for recombinant proteins, MED12L protein should be stored at −20 °C or −80 °C for long-term preservation, aliquoted to avoid repeated freeze-thaw cycles, and kept at 4 °C only for short-term use within 1–2 weeks. Always refer to the lot-specific CoA and product datasheet for exact storage conditions recommended for rp368224.

What applications is Recombinant Human MED12L Protein validated for?

Recombinant mediator subunit proteins are commonly used in protein–protein interaction studies (Co-IP, pull-down), antibody validation (Western blot, ELISA), structural biology reconstitution experiments, and drug discovery screening. However, specific validated applications for rp368224 were not detailed in the source material; please consult the official datasheet or contact Aladdin Scientific for confirmation.

How do I request a Certificate of Analysis (CoA) for rp368224?

You can request the lot-specific Certificate of Analysis for rp368224 by contacting Aladdin Scientific's customer service team with your order number and lot number. The CoA contains critical quality control data including purity assessment, protein concentration, and endotoxin levels where applicable.

References

1. Bourbon HM. Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex. Nucleic Acids Research. 2008.
2. Allen BL, Taatjes DJ. The Mediator complex: a central integrator of transcription. Nature Reviews Molecular Cell Biology. 2015.
3. Aladdin Scientific product page for Recombinant Human MED12L Protein, SKU rp368224 (absea#PP-3243).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.