Topic

Stains&Dyes

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

4 resources · protocols, FAQs & articles
protocol

Standard Operating Procedure for SYTO 9 and PI Combined Live/Dead Staining

This SOP is intended for the standardized application of SYTO 9 and PI combined live/dead staining in microbial viability evaluation. It is suitable for suspended bacterial samples such as Escherichia coli and Staphylococcus aureus, and can also be extended to membrane integrity stratification ...

Updated Apr 202612 min read✓ Reviewed
protocol

DRAQ5 and DRAQ7 Nuclear Dye Experimental Operating Procedure

This procedure is intended for the standardized use of the two far-red nuclear dyes DRAQ5 and DRAQ7. It is applicable to live-cell nuclear labeling, dead-cell identification, nuclear counterstaining of fixed samples, and immunofluorescence-associated detection on fluorescence microscopy, ...

Updated Apr 202612 min read✓ Reviewed
protocol

Fura-2 AM Staining Protocol

This protocol has been successfully adapted to different cell types, including fibroblasts, PC12 cells, and embryonic neurons, with different Fura-2 AM concentrations corresponding to different cell densities .

Updated Jul 202412 min read✓ Reviewed

Related topics

Techniques often explored alongside immunological experiments.

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