Endogenous pathological deposits are a subset of pigment deposits. Tissue cells undergo certain pathological changes to form deposits with distinct morphological characteristics. These polymerized special proteins can exhibit fibrin after staining. Fibrin, is a special protein formed by the polymerization of fibrin molecules present in the blood. It exists in tissues in the form of curved filamentous cellulose, mostly presenting a reticular structure, and sometimes forming a thick cellulose network. Old cellulose deposits can aggregate into amorphous masses.
Fibrin Staining Solution (Gram Crystal Violet Method) is mainly composed of Gram Eosin Staining Solution, Crystal Violet Staining Solution, and Gram Iodine Solution. It is a simple and cost-effective reagent for cellulose staining, which stains cellulose blue. This reagent is for research use only and not suitable for clinical diagnosis or any other purposes.
C1508743
| Component | 3×50 mL | Storage |
| C1508743A | Gram Eosin Staining Solution
| 50 mL | RT. Store in the dark.
|
| C1508743B | Crystal Violet Staining Solution
| 50 mL | RT. Store in the dark.
|
| C1508743C | Gram Iodine Solution
| 50 mL | RT. Store in the dark. |
Materials to Be Prepared by the User
1. Graded ethanol solutions, distilled water, deionized water, xylene or environment-friendly dewaxing and clearing solution, neutral mounting medium
2. Absorbent paper, microscope
Operating Procedures (For Reference Only)
1. Prepare routine paraffin sections, then perform routine dewaxing with xylene or dewaxing and clearing solution and hydrate the sections stepwise to water.
2. Immerse the sections in Gram Eosin Staining Solution for 10 minutes, followed by brief rinsing with water.
3. Transfer the sections to Crystal Violet Staining Solution for 3-5 minutes, then rinse briefly with water.
4. Immerse the sections in Gram Iodine Solution for 3-5 minutes.
5. Pour off the Gram Iodine Solution and blot the sections dry with absorbent paper.
6. Prepare a differentiation solution by mixing aniline and xylene in equal volumes, and differentiate the sections for approximately 30 seconds.
7. Rinse the tissues with fresh xylene or dewaxing and clearing solution, then mount the sections with neutral mounting medium.
8. Observe the sections under a microscope.
Staining Results
Cellulose: Blue
Background: Red
Precautions
1. After staining with Gram Eosin Staining Solution, ensure that the tissues retain sufficient red color during water rinsing.
2. When differentiating with the equal-volume mixture of aniline and xylene, shake the container gently to ensure uniform decolorization of the tissues.
3. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
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