GAPDH Mouse mAb - Anticuerpo primario, specific to GAPDH, IgG1

Cat. No.: Ab179920

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, See COA

Application

Flow Cytometry, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

B07/4G9

Conjugation

Unconjugated

Descripción general

Application
WB: 1/2,000-1/50,000 ; Predicted molecular weight: 37 kDa
Flow: 0.016 μg/mL-0.5 μg/mL
Description
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. This GAPDH antibody can be used as a loading control antibody.

Specifications

Product Name

GAPDH Mouse mAb - Anticuerpo primario, specific to GAPDH, IgG1

Sinónimos

38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

Especies hospedadoras

Ratón

Especificidad

GAPDH

Inmunógeno

Recombinant full length human GAPDH

Control positivo

WB: HEK293, HeLa, Jurkat, A431, HepG2 and K562 whole cell lysates Flow: HeLa cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotipo

IgG1

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: GAPDH Mouse mAb (Ab179920)

GAPDH Mouse mAb (Ab179920) - Western Blot
All lanes: GAPDH Antibody (Ab179920) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 35.8 kDa
Observed band size: 36.4 kDa
Exposure time: 36.9 s

Flow Cytometry: GAPDH Mouse mAb (Ab179920)

GAPDH Mouse mAb (Ab179920) - Flow Cytometry
Overlay histogram showing HeLa cells stained with GAPDH Mouse mAb (Ab179920) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Aplicación

Aplicación

Dilution info

Flow Cytometry

0.016 μg/mL - 0.5 μg/mL

WB

1/2,000-1/50,000 ; Predicted molecular weight: 37 kDa

Objetivos asociados (humanos)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)

Discover Target: GAPDH

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GAPDH Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.