GAS7 Mouse mAb - Anticuerpo primario, specific to GAS7

Cat. No.: Ab104836

CALIDAD & PUREZA See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón, Sin embargo

Isotype

Mouse IgG2a

Clone Number

OTI2F6

Conjugation

Unconjugated

Descripción general

Mouse anti Human GAS-7 Antibody, Monoclonal (OTI2F6), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Especificaciones

Product Name

GAS7 Mouse mAb - Anticuerpo primario, specific to GAS7

Sinónimos

GAS 7|GAS-7|Gas7|Growth arrest specific 7|Growth arrest-specific protein 7|KIAA0394|MLL/GAS7|MLL/GAS7 fusion protein|GAS 7 antibody|GAS-7 antibody|Gas7 antibody|Growth arrest specific 7 antibody|Growth Arrest Specific Protein 7 antibody|Growth arrest-specific protein 7 antibody|KIAA0394 antibody|MGC1348 antibody|MLL/GAS7 antibody|MLL/GAS7 fusion protein antibody

Especificaciones y pureza

See COA

Mecanismos bioquímicos y fisiológicos

May play a role in promoting maturation and morphological differentiation of cerebellar neurons.

Especies hospedadoras

Ratón

Especificidad

GAS7

Inmunógeno

Recombinant full length protein of human GAS7 produced in HEK293T.

Control positivo

WB: HEK293T transfected with pCMV6-ENTRY GAS7 cDNA for 48 hrs, HepG2, HeLa, HT29, Jurkat, MDCK, PC12, MCF7 lysates. IHC: Human adenocarcinoma of endometrium, kidney tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY GAS7. Flow Cytometry:

Conjugación

Unconjugated

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Aplicación

Aplicación

Dilution info

WB

1/500-1/2000

Flow Cytometry

1/100

IHC

1/150

IF/ICC

1:100

Objetivos asociados (humanos)

GAS7 Tbio Growth arrest-specific protein 7 (0 Activities)

Discover Target: GAS7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Tested applications, recommended dilutions, positive controls, and antigen retrieval conditions are not specified for SKU Ab104836; refer to CoA/Spec Sheet.

General guidance for initial optimization (not product specifications):

• Begin with a small panel of dilutions (e.g., for WB and IF/IHC) to identify optimal signal-to-noise under your conditions.
• Employ appropriate secondary antibodies against mouse IgG, matched to detection platform (HRP, AP, or fluorophore-conjugated). Verify cross-adsorption when working with multiple species.
• Include controls: isotype control, no-primary control, and a known GAS7-positive lysate/tissue.
• Document lot number, buffer composition, incubation times/temperatures, and wash stringency to support reproducibility.

For definitive, item-specific protocols, consult the CoA/Spec Sheet supplied with the lot.

Biological Roles

GAS7 (Growth Arrest–Specific 7) is a cytoskeleton-associated protein implicated in neuronal differentiation and morphogenesis (literature):

• Family/Domain: GAS7 belongs to the PCH/F-BAR family, proteins that bind membranes and modulate actin cytoskeleton dynamics, influencing membrane curvature and neurite extension.
• Expression: Enriched in neural tissues, particularly during development and differentiation. Expression can increase upon growth arrest in certain cell types.
• Cellular localization: Predominantly cytoplasmic with enrichment at neurites and actin-rich structures; co-localization with microtubules and F-actin reported.
• Isoforms: Multiple splice variants (e.g., GAS7a, GAS7b, GAS7c) differ in domain architecture and cellular distribution, with functional implications for neuritogenesis.
• Functions:
  • Promotes neurite outgrowth and branching by coordinating actin remodeling and microtubule stabilization.
  • May scaffold signaling components at the membrane-cytoskeleton interface to regulate neuronal morphology.
• Pathway interactions: Interfaces with actin regulatory networks; has been linked to pathways controlling growth arrest and differentiation states.

Application context for a GAS7 antibody (general):

• Mapping spatial/temporal expression during neuronal differentiation in vitro (e.g., NGF-treated PC12, iPSC-derived neurons).
• Monitoring cytoskeletal remodeling and neuritogenesis via co-staining with markers such as βIII-tubulin, MAP2, and phalloidin.

Note: Species reactivity, isoform specificity, and epitope for SKU Ab104836 are not specified; consult the CoA/Spec Sheet for any item-specific data.

Buffer Applications

This product is an antibody reagent rather than a buffering agent. Therefore, classical buffer preparation/use (buffer capacity, pH range) does not apply.

Practical notes (general):

• Working buffers: PBS or TBS at pH ~7.2–7.6 are commonly used for dilution and washes in immunoassays. Add 0.05% Tween-20 for TBST/PBST where reduced nonspecific binding is desired.
• Blocking buffers: 3–5% non-fat milk or BSA are standard for WB; serum or protein-based blockers may be chosen for IHC/IF depending on background and secondary antibody.

Item-specific buffer composition and recommended diluents are not specified for this SKU; refer to the CoA/Spec Sheet.

Green Alternatives

While this product is a biological reagent rather than a chemical solvent, environmentally mindful practices can still reduce footprint:

• Minimize single-use plastics by employing reusable racks and buffer reservoirs where sterility permits.
• Prepare buffers with concentrated stocks and validated tablets to reduce packaging waste and shipping mass.
• Choose non-toxic blocking agents and detergents (e.g., avoid excessive use of solvent-based clearing agents for IHC when aqueous alternatives suffice).
• Prefer HRP substrates and fluorophores with longer shelf-life to reduce waste from expired reagents; consolidate orders to reduce shipment frequency.
• If sodium azide is present (check CoA/Spec Sheet), collect azide-containing liquid waste separately to prevent formation of metal azides in plumbing and enable appropriate disposal.

Comparison (general):

• Traditional xylene clearing for histology vs. limonene-based or aqueous clearing systems: aqueous/limonene options can reduce VOC emissions but may require protocol adjustments and have different compatibility with mounting media.
• Methanol-based fixation vs. paraformaldehyde fixation: PFA (in fume hood) can reduce solvent VOC exposure and often preserves ultrastructure for cytoskeletal targets like GAS7, but disposal requirements apply.

Adopt institutional green lab guidelines (e.g., My Green Lab) to balance performance with sustainability.

Pharmaceutical Uses

This product is supplied strictly for research use and is not intended for human or veterinary use, diagnostics, or therapeutic applications.

In a laboratory/manufacturing context (general):

• Analytical/release testing: Research-grade antibodies can be used as in-process controls or analytical reagents in development labs (e.g., to assess expression of GAS7 in cell-based models), but not for GMP/clinical release without appropriate qualification.
• Biospecimen characterization: Utility in preclinical discovery to characterize differentiation states in neural cell models.
• Platform development: May support method development (e.g., WB, IHC) before transitioning to qualified or GMP-grade reagents.

Regulatory note: No pharmacopeial status, therapeutic claims, or diagnostic clearance are provided for this SKU. Any use in regulated workflows requires user-led qualification/validation. Item-specific formulation, purity, and lot release criteria are not specified; consult CoA/Spec Sheet for available quality data.

Physical Properties

• Appearance, concentration, buffer formulation, and preservative content: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight (item-specific): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general reference values for typical mouse IgG (for context; not product specifications):

• Approximate molecular mass: ~150 kDa for full-length IgG.
• Isoelectric point (IgG class): typically pI ~6–9 depending on isotype and glycosylation.
• Solubility: Highly soluble in aqueous physiological buffers (e.g., PBS, TBS); stability shaped by pH, ionic strength, and excipients (e.g., sugars, amino acids, proteins).
• Stability considerations: Antibodies are sensitive to repeated freeze–thaw; avoid vigorous agitation and elevated temperatures to prevent aggregation.

Note: Do not treat the literature values above as item specifications. For exact concentration, buffer composition, presence/absence of carrier proteins (e.g., BSA) or preservatives (e.g., sodium azide), and any other physicochemical details, consult the product’s CoA/Spec Sheet.

Quality and Grades

• Grade/Purity (item-specific): Not specified for this item; refer to CoA/Spec Sheet.

What quality typically means for monoclonal antibodies (general):

• Monoclonal specificity: A single B-cell–derived clone yields a uniform antibody population with consistent epitope recognition, enhancing lot-to-lot reproducibility.
• Purity metrics: Common assessments include SDS-PAGE (reducing/non-reducing) for heavy/light chain profiles, SEC-HPLC for aggregate content, and residual host-cell proteins/DNA quantitation where applicable.
• Functional validation: Performance is application-dependent (e.g., WB, IHC, IF, IP). Absent item-specific validations, users should optimize empirically under their assay conditions.
• Isotype/host: Mouse origin typically enables pairing with anti-mouse secondary antibodies across multiple detection platforms; exact isotype for this item is not specified.
• Preservatives/stabilizers: Formulation may include proteins (BSA), sugars (trehalose), or azide for stability/bioburden control—check CoA/Spec Sheet for this item.

Lot-to-lot considerations:

• Maintain consistent blocking buffers, secondary antibodies, and sample prep to minimize variability.
• For critical applications, consider aliquoting a single lot and validating under intended conditions to avoid cross-lot variability.

Reaction and Applications

As a monoclonal antibody targeting GAS7, this reagent is suited to immunoassay-based applications. Item-specific validation data are not provided; the following are general use-cases and optimization guidance for GAS7 antibodies (literature/general):

• Western blot (WB):

  • Objective: Detect GAS7 isoforms in lysates from neuronal or differentiating cells where GAS7 expression is enriched.
  • Tips: Use RIPA or mild lysis buffers to preserve isoform integrity; include protease inhibitors. Load 10–40 µg total protein per lane initially and optimize. Employ appropriate anti-mouse secondary antibodies and stringent blocking (5% milk or BSA).

• Immunofluorescence (IF)/Immunocytochemistry (ICC):

  • Fixation: Paraformaldehyde (3–4%) typically preserves cytoskeletal association; methanol fixation can alter epitope exposure—optimize empirically.
  • Permeabilization: 0.1–0.3% Triton X-100 or saponin as needed. Include counterstains for F-actin/microtubules to study GAS7 localization.

• Immunohistochemistry (IHC):

  • Tissues: Brain and neural tissues where GAS7 is expressed; antigen retrieval (citrate pH 6 or Tris-EDTA pH 9) may improve signal—optimize conditions.

• Immunoprecipitation (IP)/Co-IP:

  • Use mild non-denaturing buffers to preserve complexes with actin-associated partners.

Note: Specific dilutions, retrieval conditions, and positive controls for SKU Ab104836 are not specified; users should perform small-scale titrations and include appropriate isotype/negative controls.

Reaction Conditions

Not applicable in the chemical synthesis sense. However, for immunoassay “reaction” conditions, consider the following general, literature-based guidance (not item-specific specifications):

• Western blot: Incubate primary antibody with membrane in blocking buffer (e.g., 5% milk/TBST) at 4°C overnight or at room temperature for 1–2 h, followed by species-appropriate secondary. Optimize primary/secondary dilutions empirically.
• IF/IHC: Primary incubation typically 1–16 h at room temperature or 4°C. Antigen retrieval conditions and permeabilization should be optimized per tissue/cell type.
• IP: Incubate clarified lysate with antibody for 1–4 h at 4°C with rotation; capture using Protein A/G resin compatible with the antibody isotype (isotype for this item is not specified—test A vs. G or consult CoA/Spec Sheet).

For SKU Ab104836, no validated conditions, dilutions, or controls are provided. Users should perform pilot titrations and include appropriate positive/negative and isotype controls.

Safety and Handling

• Research use: For research use only.
• GHS/CLP classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS for authoritative safety information.
• Primary hazards: Proteins/antibodies generally present low acute toxicity but may cause allergic reactions or irritation upon exposure; handle as a biological reagent.
• Preservatives: Some antibody formulations contain sodium azide or thimerosal. Presence/amount for this item is not specified; check the CoA/Spec Sheet. If sodium azide is present, avoid contact with copper/lead plumbing (azide can form explosive metal azides in drains) and follow appropriate disposal protocols.
• Recommended PPE: Lab coat, nitrile gloves, and safety glasses. Work in a clean area to minimize microbial contamination.
• Handling:
  • Avoid repeated freeze–thaw cycles (see Storage & Reconstitution).
  • Do not vortex harshly; mix by gentle inversion to limit aggregation/denaturation.
  • Keep on ice during use if supplied as a liquid to maintain stability.
• Incompatibilities: Strong oxidizers and extreme pH can denature proteins; avoid adsorption to untreated glass/plastic by using low-bind tubes when possible.
• First aid (general): In case of skin/eye contact, rinse with water. If inhaled or ingested, seek medical attention. Provide SDS to medical personnel.
• Spill/leak: Absorb with inert material, clean with aqueous detergent, and dispose according to institutional and local regulations.
• Always consult the product SDS for definitive safety, handling, and disposal guidance.

Solvent Selection

This product is an aqueous protein reagent (antibody), not an organic chemical or solvent. Traditional “solvent selection” does not apply.

Practical notes for buffer compatibility (general):

• Compatible media: Physiological buffers such as PBS or TBS are commonly used. The optimal pH is typically near neutral (pH 7.2–7.6) to preserve IgG stability.
• Additives: Detergents (e.g., 0.05% Tween-20) can reduce nonspecific binding in immunoassays; sugars (trehalose/sucrose) and proteins (BSA) may stabilize during storage. Presence/amounts for this item are not specified—consult CoA/Spec Sheet before adding or diluting.
• Avoid: Strong organic solvents, extreme pH, and high shear; these may denature or aggregate antibodies.
• Filtration: If needed, use low-protein–binding membranes (e.g., PES, PVDF) to minimize loss.

If you require chemical solvent guidance (e.g., for tissue processing or epitope retrieval), refer instead to the Reaction & Applications and Application Protocols sections along with your institutional SOPs.

Storage and Reconstitution

• Storage conditions (item-specific): Store at -20°C. Avoid repeated freezing and thawing.
• Shipping (item-specific): Shipped in an ice chest with ice pads.

Working practices (general):

• Aliquoting: Upon first receipt/thaw, aliquot into single-use portions to prevent freeze–thaw cycles. Use low-bind tubes.
• Short-term storage: If supplied as liquid and compatible with the formulation, 2–8°C storage may be suitable for short periods (days to weeks); check CoA/Spec Sheet for this item.
• Long-term storage: -20°C as specified; some antibodies perform better at -80°C for multi-year storage—user may evaluate as needed.
• Freeze–thaw: Minimize to preserve activity and prevent aggregation. Thaw on ice and mix gently.
• Light sensitivity: Protect from prolonged light if conjugated; conjugation status for this item is not specified.
• Reconstitution: If supplied lyophilized (status not specified), reconstitute with sterile, antibody-grade water or buffer as directed on the CoA/Spec Sheet, then allow to equilibrate gently on ice. Avoid vigorous agitation.
• Dilution: Use compatible buffers (e.g., PBS/TBS with carrier protein if recommended). Filter sterilize with low-protein–binding membranes only if necessary.

Always consult the lot-specific CoA/Spec Sheet for exact formulation, concentration, and handling instructions.

Structure and Identity

GAS7 Mouse mAb is a mouse-derived monoclonal antibody raised against the GAS7 (Growth Arrest-Specific 7) protein target.

• Item-specific identifiers:

  • SKU: Ab104836
  • Antibody type: Mouse monoclonal antibody (mAb) against GAS7
  • Clone, isotype, light chain type, and epitope: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES: Not applicable to antibodies.

• Molecular characteristics (item-specific):

  • Molecular formula, exact molecular weight, sequence: Not specified for this item; refer to CoA/Spec Sheet.

• Structural/biochemical features (general, literature):

  • Typical IgG mAbs are heterotetramers (2 heavy chains + 2 light chains) with disulfide bonds and conserved Fc region; variable regions (VH/VL) define antigen binding.
  • Common isotypes for mouse mAbs include IgG1, IgG2a, IgG2b, IgG3, and IgM; exact isotype for this product is not provided.
  • Approximate mass for a full-length IgG is ~150 kDa (literature).

Synthetic Utility

Not applicable. This product is a biological monoclonal antibody reagent and is not used as a synthetic building block or reagent in chemical synthesis. For practical laboratory guidance, see Reaction & Applications and Application Protocols.

Target Specificity

• Antigen: GAS7 (Growth Arrest–Specific 7)
• Species reactivity (tested), cross-reactivity, and epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Clone designation and isotype (e.g., IgG1, IgG2a): Not specified for this item; refer to CoA/Spec Sheet.
• Applications tested/validated: Not specified for this item; refer to CoA/Spec Sheet.

General notes (literature): GAS7 is a cytoskeleton/membrane-associated protein involved in neuritogenesis. Antibody performance can vary by isoform and sample prep; confirm target band size and staining pattern with appropriate controls.

Preguntas frecuentes

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GAS7 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.