GBP2 Mouse mAb - Anticuerpo primario, specific to GBP2

Cat. No.: Ab104951

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

OTI4E7

Conjugation

Unconjugated

Descripción general

Mouse anti Human GBP2 Antibody, Monoclonal (OTI4E7), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

GBP2 Mouse mAb - Anticuerpo primario, specific to GBP2

Sinónimos

GBP 2 antibody | GTP binding protein 2 antibody | Guanine nucleotide binding protein 2antibody | Guanylate binding protein 2 interferon inducibleantibody | HuGBP 2 antibody | HuGBP2 antibody | Interferon induced guanylate binding protein 2 antibody | GBP2

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP. Followin

Especies hospedadoras

Ratón

Especificidad

GBP2

Inmunógeno

Recombinant full length protein of human GBP2.

Control positivo

WB: HeLa, A549, MCF7 cell lysates. IHC: Human colon, colon adenocarcinoma, ovary, pancreas, endometrium, prostate, bladder, bladder carcinoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY GBP2. Flow Cytometry: HEK293T cells transfec

Conjugación

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - Western Blot
All lanes: GBP2 Mouse mAb (Ab104951) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 68 kDa
Exposure time: 90.0 s

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human colon tissue using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using GBP2 Mouse mAb (Ab104951). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IHC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using GBP2 Mouse mAb (Ab104951) . (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab104951)

IF/ICC: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - IF/ICC
GBP2 Mouse mAb (Ab104951) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2.

Flow Cytometry​: GBP2 Mouse mAb (Ab104951)

GBP2 Mouse mAb (Ab104951) - Flow Cytometry​
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by GBP2 Mouse mAb (Ab104951), and then analyzed by flow cytometry.

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 ;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/50

Objetivos asociados (humanos)

GBP2 Tbio Guanylate-binding protein 2 (0 Activities)

Discover Target: GBP2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GBP2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.