GRADE & PURITYMoligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
This compound belongs to the class of organic compounds known as imidazopyridines. These are organic polycyclic compounds containing an imidazole ring fused to a pyridine ring. Imidazole is 5-membered ring consisting of three carbon atoms, and two nitrogen centers at the 1- and 3-positions. Pyridine is a 6-membered ring consisting of five carbon atoms and one nitrogen center.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
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Application Protocols
Item-specific, catalog-verified protocols: Not specified for this item.
General guidance for small-molecule tool use (not item-specific)
Stock solution: Prepare 10 mM in DMSO; aliquot and store to avoid repeated freeze–thaw.
Biochemical kinase assay (example framework):
Buffer: 50 mM HEPES pH 7.4, 10 mM MgCl2, 0.01% Tween-20, 1 mM DTT.
Preincubate enzyme with compound for 10–30 min at room temperature; initiate with ATP/substrate.
Maintain final DMSO ≤0.5% v/v; include vehicle and positive control wells.
Readout: ADP-Glo, radiometric, or TR-FRET as validated.
Cell-based assay framework:
Treat cells with serial dilutions (e.g., 10-point, 3-fold) for 0.5–2 h.
Harvest for phospho-protein analysis (e.g., p-ERK Western blot) or phenotypic readouts.
These outlines are generic starting points. Optimize concentrations, exposure times, and detection modalities for your system.
Biological Roles
Item-specific biological claims: None provided in the Product Data. No claims are made here about potency, selectivity, or activity for this specific catalog item.
Literature/general context (not item-specific)
GDC-0623 is described in the literature as a small-molecule inhibitor used to interrogate the MAPK/ERK pathway at the level of MEK1/2 (MAP2K1/2). In cellular systems, tool compounds of this class are employed to modulate ERK phosphorylation and to study feedback regulation within RAF–MEK–ERK signaling.
Typical experimental roles include: validating pathway dependence in cell models, benchmarking with orthogonal chemotypes, and supporting phospho-protein biomarker development (e.g., p-ERK readouts by Western blot or AlphaLISA).
Off-target profiling (kinome scans, safety panels) is commonly used in publications to contextualize selectivity; consult primary literature for specific datasets and assay conditions.
Practical considerations for bioassays
Establish DMSO tolerance for each assay system and confirm compound solubility at the top test concentration (inspect for precipitation, use light scattering if available).
Include positive and negative controls, time-course sampling for pathway dynamics, and washout studies to assess reversibility.
For quantitative work, verify stock concentration by qNMR or calibrated UV to reduce variability in IC50/EC50 determinations.
All biological work should be conducted under “research use only” conditions with appropriate institutional approvals.
Buffer Applications
Not typically applicable. GDC-0623 is a small-molecule screening compound and not a buffering agent.
Practical notes for assay buffers (general guidance)
Common buffers for kinase/cell assays: HEPES, Tris, or PBS at pH 7.2–7.5; include Mg2+/ATP and cofactors as required by the enzyme system.
Maintain final DMSO content (typically ≤0.1–1% v/v) to balance solubility and biological tolerance.
Filter-sterilize working solutions when used in cell culture; verify compound stability over assay duration.
No buffer capacity, pKa, or buffer recipes are provided or implied for this item.
Green Alternatives
This section is less directly applicable because GDC-0623 is a discrete screening compound rather than a process solvent or bulk reagent where greener substitutions are typically evaluated.
General green chemistry considerations for handling small-molecule tools
Minimize DMSO volumes by preparing concentrated stocks and performing accurate serial dilutions to reduce solvent waste.
Prefer aqueous assay buffers with low organic content when feasible; validate compound solubility and activity.
For cleaning and sample prep, replace chlorinated solvents with alcohols or acetone where method-permissive.
Comparison of common stock solvents (general guidance)
DMSO: Excellent solvency, miscible with water; relatively benign but enhances dermal absorption—use strict PPE.
DMF: Strong solvency but with higher regulatory scrutiny; avoid when DMSO suffices.
Acetonitrile: Volatile and suitable for LC; flammable and toxic—use in hood, recover via solvent recycling if available.
Operational practices
Use micro-aliquoting to avoid repeated freeze–thaw cycles and reduce discarded material.
Implement solvent capture and recycling programs in HTS labs where volumes justify recovery.
Note: There is no greener “substitute” for the compound itself; greenness pertains to how it is formulated, delivered, and disposed.
Pharmaceutical Uses
Item-specific pharmacopeial status: Not specified for this item; refer to CoA/Spec Sheet. Supplied strictly for research use only.
General, non-clinical context (not item-specific and with no therapeutic claims)
In pharmaceutical R&D settings, pathway tool compounds like GDC-0623 may be used as internal reference standards, controls in signaling assays, or comparators in SAR campaigns. They can support method development (LC–MS bioanalytical calibrators), permeability assessments, and stability studies.
Formulation research may explore solubilization strategies (e.g., cyclodextrins, lipid vehicles) for in vivo preclinical model work; any such use must be supported by institution-specific approvals and is outside the scope of this catalog.
Compliance notes
Not for human or veterinary use. Not a drug substance or excipient. No claims are made regarding GMP status, pharmacopoeial monographs, or clinical suitability.
For regulated studies, procure appropriate GMP-grade materials and definitive reference standards; verify identity/purity with compendial or validated methods.
Physical Properties
Item-specific (from Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Aggregated physicochemical parameters for MEK-pathway tool compounds generally fall into: MW ~350–550 Da, cLogP ~2–5, HBD 0–2, HBA 5–9, and polar surface area ~60–110 Ų. These ranges balance cell permeability with solubility; they are not specific to this product and must not be used as specifications.
Solubility practice: Many screening compounds dissolve well in anhydrous DMSO (stock solutions 10–50 mM). Aqueous solubility commonly requires cosolvent (DMSO 0.1–1% v/v) or surfactant systems for biochemical assays. Always determine solubility experimentally for the exact lot.
Spectral characterization typically uses 1H/13C NMR, HRMS/ESI, and HPLC/UPLC purity assessment; UV–Vis profiles vary with chromophores.
Unavailable/unspecified values for this catalog item
Melting point, boiling point, density, refractive index, pKa, logP/logD, UV cut-off: Not specified for this item; refer to CoA/Spec Sheet.
Water content, residual solvents, metal content: Not specified for this item; refer to CoA/Spec Sheet.
Note: Where exact numerical values are required for method development, please consult the item’s CoA/Spec Sheet or acquire them experimentally on the received lot.
Quality and Grades
Item-specific details
Grade/Purity: Moligand™ (as listed). No numeric purity or stabilizers are specified for this item; refer to CoA/Spec Sheet for lot-specific data.
About Moligand™ grade (general description; not a specification)
Moligand™ designates small-molecule ligands supplied for screening, probe development, and SAR exploration. Typical expectations for library-grade compounds include analytical confirmation of identity (NMR/MS) and chromatographic purity suitable for discovery workflows, but exact test methods and thresholds vary by supplier and should be verified on the item’s CoA.
UV background and LC/MS compatibility: Library-grade materials are generally amenable to UHPLC/LC–MS for QC. If your application is bioassay or HTS, review the reported purity basis (e.g., HPLC area %, single wavelength vs. PDA) and counterion/solvate content.
Practical recommendations
Request or download the CoA to confirm: purity basis and percent, residual solvent profile, water content, salt/solvate form, and identity data (HRMS, NMR).
If quantitative work is planned (e.g., IC50/Ki determination), we recommend independent verification of purity and concentration (qNMR or calibrated UV) to minimize assay-to-assay variance.
Reaction and Applications
Manufacturer applications (from catalog)
Not specified for this item beyond “For research use only.”
Practical research uses (general, not item-specific specifications)
Screening/profiling: GDC-0623 is widely referenced as a small-molecule tool compound in MAPK pathway studies (biochemical and cellular). Typical applications include target engagement assays, pathway phosphorylation readouts, and SAR benchmarking alongside related chemotypes. Verify identity and assay-grade purity before quantitative comparisons.
Reference standard: Useful as a chromatographic or bioassay reference when building internal panels of pathway modulators. For LC–MS, prepare accurately weighed DMSO stocks and store aliquots to minimize freeze–thaw.
Analytical and formulation notes
Assess compound stability in DMSO and in aqueous buffers (pH 6–8) over relevant time frames (hours to days). Use LC–MS to monitor degradation/adsorption.
If solubility is limiting, employ staged dilution (DMSO → buffer with vigorous mixing) and consider inclusion of low nonionic surfactant if assay-compatible.
Not typically used as a synthetic reagent
As a finished tool compound, GDC-0623 is not commonly leveraged as a building block or catalyst; see “Synthetic Utility” for caveats if derivatization or bioconjugation is intended.
Reaction Conditions
Not directly applicable, as GDC-0623 is offered as a finished screening compound rather than a reagent or catalyst.
General laboratory handling of solutions (not item-specific reaction data)
Stock preparation: Dissolve in anhydrous DMSO to 10–50 mM. Mix thoroughly (vortex/sonicate briefly), then sterile-filter (0.22 µm) if needed for cell-based assays.
Aqueous dilution: Add DMSO stock to vigorously stirred or pipette-mixed buffer to avoid localized supersaturation. Aim for final DMSO ≤0.1–1% v/v.
Stability checks: Use LC–MS or HPLC to assess stability at room temperature and 4 °C over 24–72 h in your specific buffer system. Protect from light if chromophores are present (unknown for this item; take conservative precautions).
If chemical modification is attempted (requires known structure)
Typical conditions for heteroaryl couplings (literature, not specific): Pd-catalyzed Suzuki–Miyaura (ligated Pd(0), 50–100 °C, aqueous organic solvents), Buchwald–Hartwig aminations (Pd/BINAP or dialkylbiaryl phosphines), or SNAr in polar aprotic solvents at 60–120 °C. These are scaffold-dependent and provided only as general references.
Safety and Handling
Item-specific hazard information (from Product Data)
Signal word: Not specified for this item; refer to SDS.
H-statements: Not specified for this item; refer to SDS.
GHS classification/pictograms: Not specified for this item; refer to SDS.
General laboratory safety guidance for small-molecule research compounds (not item-specific)
Handle in a chemical fume hood; avoid inhalation and contact with skin/eyes. Wear appropriate PPE: lab coat, safety glasses, and chemically resistant gloves (e.g., nitrile). Consider double-gloving for DMSO solutions given enhanced dermal uptake.
Avoid ingestion and aerosol generation. Use secondary containment for solutions and clearly label concentration and solvent.
Storage incompatibilities: Keep away from strong oxidizers and strong acids/bases until specific compatibility is known. Protect from moisture if the compound is hygroscopic and from light if chromophoric; verify with CoA.
First aid (overview; defer to SDS): Eye/skin contact—rinse with water for at least 15 minutes; remove contaminated clothing. Inhalation—move to fresh air. Ingestion—rinse mouth; seek medical attention. Provide SDS to responders.
Spill response: Absorb small spills with inert material (vermiculite), collect in a suitable waste container. Decontaminate area, ventilate, and dispose per institutional chemical waste procedures.
Always consult the product-specific SDS for authoritative hazard, toxicological, and disposal information before use.
Solvent Selection
Item-specific solubility data: Not specified for this item; refer to CoA/Spec Sheet and test empirically on your lot.
General guidance for small-molecule screening compounds (not item-specific)
Primary stock solvent: Anhydrous DMSO is typically preferred due to broad solvating power and assay compatibility. Common stock concentrations: 10–50 mM; filter-sterilize if required for cell-based assays (0.22 µm PTFE or PVDF compatible with DMSO).
Secondary solvents: DMF, acetonitrile, ethanol, or isopropanol may be viable. Evaluate stability and assay interference (e.g., ACN in enzyme kinetics; alcohols in membrane assays).
Aqueous use: Prepare working solutions by serial dilution into assay buffer, keeping final DMSO ≤0.1–1% v/v depending on biological tolerance. Add cosolvents/surfactants (e.g., 0.01–0.05% Tween-20) only if validated for your assay.
Polarity and miscibility: DMSO is fully miscible with water and many organics; perform solubility checks at intended temperatures. Monitor for precipitation on chilling or upon buffer addition.
Choosing among alternatives
If photosensitivity or baseline UV absorbance is a concern, consider DMF or ACN for analytical prep and run PDA blanks to assess background.
For crystallography or biophysical assays (SPR, ITC), prioritize buffers with minimal organic content and pre-validate compound solubility and aggregation behavior (DLS or light scattering).
Storage and Reconstitution
Item-specific conditions (from Product Data)
Storage: Room temperature.
Shipped in: Not specified for this item.
Reconstitution guidance (general; not item-specific specifications)
Recommended stock solvent: Anhydrous DMSO. Typical stock concentration: 10–50 mM.
Technique: Allow vial to equilibrate to room temperature before opening to minimize moisture condensation. Weigh accurately, dissolve with gentle vortexing; brief sonication may assist.
Aliquoting: Dispense into inert, tightly sealed microtubes (e.g., amber PP or glass) to minimize headspace and adsorption. Avoid repeated freeze–thaw; use single-use aliquots.
Storage of solutions (general)
DMSO stocks: Store at −20 °C or −80 °C, protected from light. Verify stability periodically by LC–MS/HPLC; discard if precipitation or degradation is observed. If stored at room temperature per specific protocol, confirm stability empirically.
Aqueous working solutions: Prepare fresh for each experiment. If short-term storage is necessary, keep at 2–8 °C, protected from light, and verify stability prior to use.
Additional notes
No stabilizers, salt form, or hydrate/solvate state are specified for this item; consult the CoA/Spec Sheet for lot-specific details.
For best reproducibility, document lot number, exact concentration (gravimetric), solvent batch, and storage history in your ELN.
Structure and Identity
Concise identity summary for this screening compound entry.
Item-specific (from Product Data)
Product name: GDC-0623 (SKU: G610532)
Catalog category: Small Molecules & Compound Libraries (小分子和化合物库)
CAS No.: G610532 (as listed; not a standard CAS format)
PubChem CID: 42642654
InChIKey: 315358 (as listed; not a standard 27-character InChIKey)
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Literature/general notes (for orientation only; not item specifications)
GDC-0623 is commonly described in the literature as a small-molecule inhibitor used to interrogate the MAPK/ERK signaling cascade at the level of MEK1/2. Exact structural descriptors (SMILES/InChIKey) should be verified from primary databases before use in regulated contexts.
Typical features for molecules in this class include multiple heteroatoms (N, O), one or more fused/aromatic ring systems, and functional groups that confer kinase hinge-binding and allosteric interactions. Stereochemistry is often not central (frequently achiral), but this must be confirmed case-by-case.
2D structure description (general)
Anticipated as a heteroaromatic, polycyclic scaffold bearing hydrogen-bond donor/acceptor motifs and a lipophilic surface suitable for cell permeability. Confirm actual structural features from an authenticated reference structure before drawing or modeling.
Synthetic Utility
This compound is supplied as a finished small-molecule tool; it is not primarily intended as a synthetic building block.
General considerations if derivatization is contemplated (not item-specific)
Without a confirmed structure, generic modifications (prodrugging, biotinylation, handle installation) cannot be advised. Confirm the exact functional groups and reactive sites from an authenticated structure before planning transformations.
If a heteroaromatic, amide/urea, or ether functionality is present (typical in kinase tools), common transformations include N-alkylation/acylation, Suzuki/Negishi cross-coupling on aryl halides, or late-stage SNAr where activated rings exist. Reaction feasibility is highly scaffold-dependent.
Use as an analytical standard
The most reliable “synthetic” role is as a calibration/retention-time standard in LC–MS or as a control in bioassays. Ensure accurate weighing (use microbalance), and prepare gravimetrically standardized DMSO stocks.
Because the exact reactive handles are not provided for this item, do not plan synthetic campaigns without obtaining the complete structure and reactivity profile from validated sources.
Target Specificity
Item-specific, catalog-verified target data: Not specified for this item.
Policy note
In keeping with catalog rules, target, potency, selectivity, clone/epitope, or species reactivity information is only reported when explicitly provided in the Product Data. Please consult primary literature and your internal validation for any biological claims you intend to make.
Preguntas frecuentes
What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
How should this product be stored?
Store at room temperature.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is G610532. InChIKey RFWVETIZUQEJEF-UHFFFAOYSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
