GI-560192 - ≥98% , CAS No.301326-41-0

CAS: 301326-41-0 Cat. No.: G416718 Fórmula: C₂₀H₁₆N₂O₂ Peso molecular: 316.35

GRADE & PURITY ≥98%

Synonyms

N'-([1,1'-Biphenyl]-4-ylmethylene)-4-hydroxybenzohydrazide

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

anti-cancer

Specifications

Sinónimos

N'-([1,1'-Biphenyl]-4-ylmethylene)-4-hydroxybenzohydrazide

Especificaciones y pureza

≥98%

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Tipo de acción

MODULATOR

Pureza

≥98%

Propiedades del producto

• ALogP: 3.959
• hba_count: 2
• Recuento HBD: 2
• Enlace rotable: 4

Nombres e identificadores

• Pubchem Sid: 504764324
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504764324
• Sonrisas canónicas: C1=CC=C(C=C1)C2=CC=C(C=C2)C=NNC(=O)C3=CC=C(C=C3)O
• IUPAC Name: 4-hydroxy-N-[(E)-(4-phenylphenyl)methylideneamino]benzamide
• InChIKey: PNAOAWLPWZRVCK-KGENOOAVSA-N
• INCHI: 1S/C20H16N2O2/c23-19-12-10-18(11-13-19)20(24)22-21-14-15-6-8-17(9-7-15)16-4-2-1-3-5-16/h1-14,23H,(H,22,24)/b21-14+
• Peso molecular: 316.35
• Reaxy-Rn: 24576172
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=24576172&ln=

Propiedades químicas y físicas

• DMSO (mM) Solubilidad máxima: 10

Application Protocols

No tested applications are provided for this item. The following is general guidance for handling small-molecule research compounds; adapt to your assay and consult the CoA/SDS.

General DMSO stock and plate protocol (not item-specific)

1. Equilibrate vial to room temperature in a desiccator to avoid moisture condensation.
2. Open vial and promptly prepare a concentrated stock (e.g., 10–50 mM) in anhydrous DMSO; vortex and, if needed, sonicate briefly.
3. Pass the solution through a PTFE syringe filter only if particulates persist and the compound is known to be stable/soluble at working concentration.
4. Aliquot stock into low-bind tubes (10–50 µL) to minimize freeze–thaw; store at -20°C.
5. For assays, thaw an aliquot once, dilute into assay buffer/media to the desired final concentration ensuring constant final DMSO (≤0.1–1% v/v).
6. Inspect for precipitation after dilution. If observed, increase organic cosolvent slightly or reduce compound concentration; mix gently to avoid bubbles.
7. Record batch/lot, stock concentration, solvent lot, and dilution scheme for reproducibility.

For analytical method development, prepare a calibration series in matched matrix and evaluate linearity, carryover, and stability (bench-top, autosampler, freeze–thaw).

Biological Roles

Item-specific biological information

• No target class, pathway, or mechanism data are provided for GI-560192 in the current product listing.

General considerations (not item-specific; for research use only)

• Small molecules like GI-560192 are commonly evaluated in vitro for binding to enzymes, receptors, or protein–protein interfaces. Without disclosed structure or annotations, no assumptions about biological role, potency, or selectivity can be made.
• If used as a putative probe:
  • Establish on-target activity and orthogonal confirmation (e.g., biochemical IC50, biophysical Kd via ITC/SPR, and cellular phenotype rescue studies where applicable).
  • Assess off-target liabilities via counter-screens and broad profiling panels as appropriate to the research aim.
• ADME/T and safety pharmacology are outside the scope of this product; do not use for diagnostic or therapeutic purposes.

Please consult your internal data, literature associated with CAS 301326-41-0 / CID 6898894, and our CoA/SDS to define any biological handling precautions and assay relevance.

Buffer Applications

This product is not a buffering agent. However, small-molecule stocks are often introduced into buffered systems for in vitro assays.

Practical guidance (general; not item-specific)

• Stock preparation: Dissolve in DMSO or ethanol to 10–50 mM, then dilute into buffer immediately before use. Common assay buffers include Tris, HEPES, or PBS at pH appropriate for the biological target.
• Control the final organic solvent: Keep DMSO/EtOH ≤0.1–1% v/v; match solvent content across controls and treated samples.
• Order of addition: Add compound last, with rapid mixing, to minimize localized supersaturation and precipitation.
• Solubility troubleshooting:
  • Increase ionic strength or add minimal surfactant (e.g., 0.01–0.05% Tween 20/80) if compatible with the assay readout.
  • Adjust pH only if the compound has ionizable groups and remains stable within that pH window (verify experimentally).
• Filtration: If particulate matter persists and the compound is stable, pass through 0.22–0.45 µm PTFE filters; avoid adsorptive membranes if losses are observed.

Document final buffer composition, solvent content, and any additives to ensure reproducibility.

Green Alternatives

Given the absence of a defined use-pattern for GI-560192 (reagent vs analyte), solvent and process choices should follow green chemistry best practices where compatible with your application.

General greener solvent guidance (not item-specific)

• For dissolution and handling:
  • Prefer ethanol, isopropanol, or water/biobased co-solvent systems when they meet solubility and assay constraints.
  • Reserve DMF, NMP, and chlorinated solvents for cases where benign options fail; document justification.
• For analytical workflows:
  • Use MeOH or MeCN sparingly; optimize gradient methods to minimize solvent use; employ smaller ID columns and UHPLC where feasible.
• For workup/purification of any derivative chemistry:
  • Replace dichloromethane with ethyl acetate or MTBE when practicable; consider 2-MeTHF or CPME instead of THF/Et2O.

Illustrative comparison (general)

• Ethanol vs DMF for stock prep: Ethanol is greener and easier to dispose of, but has lower solvating power; DMF dissolves more structures but raises EHS concerns.
• 2-MeTHF vs THF (if synthetic derivatization is attempted): 2-MeTHF offers better safety and renewable sourcing; note differences in polarity and peroxide formation tendencies.

Always ensure that greener substitutions do not compromise compound stability, solubility, or the scientific question at hand.

Pharmaceutical Uses

No excipient role, pharmacopeial status, or formulation use is specified for GI-560192. This material is supplied strictly for research use only and is not intended for human or veterinary use, clinical diagnosis, or as an API.

General information (not item-specific)

• In pharmaceutical R&D settings, research chemicals may be employed as analytical reference standards, spike/recovery controls, or internal standards in method development. Such use requires independent qualification of identity and purity and robust stability data.
• If a formulation-like study is needed (e.g., for in vitro delivery), consider:
  • Preparing aliquoted DMSO stocks and diluting into acceptable vehicles (PEG400, Captisol, or micellar systems) for nonclinical experiments only.
  • Evaluating compound stability under stress (light, heat, oxidative) to understand handling constraints.

No claims are made regarding regulatory status, GMP suitability, or pharmacopoeial monographs for this item.

Physical Properties

Item-specific (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Key physicochemical parameters

• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa(s): Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/good practice; not item-specific)

• Many small-molecule research compounds are handled as solids and dissolved initially in DMSO to prepare 10–50 mM stocks, then diluted into aqueous buffers or cell media; keep final DMSO ≤0.1–1% v/v in bioassays to minimize solvent effects.
• If aqueous solubility is low, cosolvent systems (DMSO:buffer, ethanol:buffer) or surfactants (e.g., 0.01–0.05% Tween 80) may aid dissolution; verify that additives do not interfere with your assay readout.
• For chromatography, start with reverse-phase LC conditions (C18, H2O/MeCN or H2O/MeOH + 0.1% formic acid or ammonium acetate) and optimize based on observed retention and ionization.

Quality and Grades

Item-specific (from Product Data)

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

What the grade implies (general guidance)

• If provided, research-grade small molecules typically include a purity specification determined by HPLC/UPLC, GC, or quantitative NMR. “≥98% (HPLC)” often refers to UV-integrated area percent at a specified wavelength and method.
• For bioassay use, low residual solvents, defined counterions/salt form, and controlled polymorph/solvate state can be important. Verify these on the CoA or request additional documentation if needed.

Batch-to-batch documentation

• Lot-specific CoA should include: analytical method for purity, chromatogram, identity confirmation (MS/NMR), and water/volatile content where applicable. If any of these are critical to your workflow, request them prior to use.

Stabilizers/additives

• Stabilizer presence is not specified for this item. If stabilizers are used in any lot, they will be declared on the CoA/label. Stabilizers can impact UV baselines and bioassays; adjust methods accordingly.

Fit-for-purpose recommendations

• For analytical standards, consider verifying purity by orthogonal techniques (qNMR vs HPLC area%). For biological assays, confirm endotoxin is not a concern (most small molecules are inherently low, but verify if required by SOPs).

Reaction and Applications

Item-specific applications

• Manufacturer Applications: Not specified in the product data.

General usage context (not item-specific)

• GI-560192 is listed as a research-use small molecule. In typical laboratories, such compounds are employed as:
  • Reference or control compounds in biochemical/biophysical assays.
  • Chemical matter in screening cascades (e.g., HTS, fragment/lead evaluation) and SAR exploration.
  • Analytical spikes/standards to validate LC–MS detection and sample preparation workflows.
• If the compound is a salt or contains hydrolyzable motifs, consider preparing fresh solutions and minimizing exposure to moisture and light.

If using in reaction discovery (generic guidance)

• Although not supplied as a reagent, researchers may sometimes derivatize screening compounds for SAR. If so, confirm functional handles (amines, acids, halides) via NMR/MS before attempting transformations, and expect yields to be substrate-specific.

Documentation

• Because no specific reactivity, target class, or application notes are provided, consult primary literature associated with CAS 301326-41-0 or PubChem CID 6898894 for any peer-reviewed use cases prior to experimental deployment.

Reaction Conditions

Specific reaction conditions are not applicable because GI-560192 is not provided as a reagent for named transformations, and no functional group information is disclosed.

If derivative chemistry is attempted (general, not item-specific)

• Solvents: Start with mild, anhydrous media (MeCN, THF, toluene, DCM alternatives like EtOAc/2-MeTHF when possible) guided by observed solubility and stability.
• Temperatures: Begin at ambient temperature; escalate cautiously while monitoring for decomposition.
• Catalysts/bases/acids: Choose non-nucleophilic bases (DIPEA, Hünig’s base) or weak acids first; avoid strong reagents until stability is known.
• Times/yields: Highly substrate-dependent; establish via time-course sampling (UPLC–MS). Document impurity profiles and mass balance.
• Workup/purification: Favor minimal aqueous workup if hydrolysis is suspected; use reverse-phase prep-LC for complex mixtures.

Because item-specific conditions are not available, rely on exploratory screening and analytical control tailored to the compound’s verified structure.

Safety and Handling

Item-specific (from Product Data)

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.
• Storage: Store at -20°C. Shipped in ice chest with ice pads.

General laboratory precautions (not item-specific; follow your SDS)

• Handle in a chemical fume hood with appropriate PPE: lab coat, safety glasses, and nitrile gloves. Avoid inhalation, ingestion, and skin/eye contact.
• Prevent exposure to moisture and excessive heat; keep container tightly closed. Warm to room temperature before opening to minimize condensation ingress.
• Unknown hazard profile: Treat as potentially harmful. Avoid aerosolization and dust formation; use containment during weighing.
• Incompatibilities (general): Strong oxidizers and strong acids/bases may degrade typical organic compounds; segregate accordingly. Avoid sodium/peroxide-forming practices unless specifically known to be stable.
• First aid (general): In case of skin/eye contact, rinse with water for at least 15 minutes; remove contaminated clothing. If inhaled, move to fresh air. If swallowed, rinse mouth. Seek medical attention in all exposure cases.
• Spill response: Absorb with inert material, collect in chemical waste. Decontaminate area per institutional EHS guidance.
• Waste disposal: Dispose of contents/container in accordance with local/regional regulations; label as research chemical waste.

Solvent Selection

Item-specific status

• Solubility profile and solvent preferences: Not specified for this item; refer to CoA/Spec Sheet.

General approach for small-molecule dissolution (not item-specific)

• Prepare a concentrated DMSO stock (e.g., 10–50 mM), then dilute into the working medium. Maintain final DMSO ≤0.1–1% v/v in biochemical/cell-based assays.
• If DMSO is unsuitable, screen common solvents in a tiered manner:
  • Polar aprotic: DMF, NMP, acetonitrile.
  • Protic: ethanol, isopropanol, methanol (verify assay compatibility).
  • Aqueous co-solvent systems: 10–40% solvent in buffer; adjust pH if ionizable groups are present (pH 2–10 as appropriate for stability).
• For LC/MS, use MeCN/H2O or MeOH/H2O with volatile modifiers (0.1% formic acid, 5–20 mM ammonium acetate) to balance chromatographic retention and ionization.

Practical tips

• Warm gently (25–40°C) and vortex/sonicate to aid dissolution; avoid overheating. Filter sterilize only if known to be stable and soluble at working concentration.
• Watch for precipitation after dilution into aqueous media; if observed, increase organic content slightly or reduce concentration.

Quick comparison (general)

• DMSO: maximal solvating power; high bp; widely biocompatible at ≤0.5%.
• Ethanol: good miscibility with water; evaporates readily; may affect cells at ≥0.5–1%.
• DMF/NMP: strong solvents; consider toxicity and downstream compatibility.

Storage and Reconstitution

Item-specific (from Product Data)

• Storage: Store at -20°C.
• Shipping: Ice chest with ice pads.

General storage best practices (not item-specific)

• Keep the container tightly closed in a dry, inert environment. If feasible, store under desiccation and protect from light until stability is known.
• After first opening, promptly prepare single-use aliquots to avoid repeated freeze–thaw cycles. Record open date and aliquot map.
• Avoid long-term storage in frost-free freezers due to temperature cycling; prefer a manual-defrost unit.

Reconstitution guidance (general)

• Solvent choice: Start with anhydrous DMSO for maximal solubility. Alternative solvents (ethanol, MeCN, DMF) may be considered depending on downstream use; confirm compatibility.
• Concentration: Typical lab stocks are 10–50 mM; use amber vials if light sensitivity is suspected.
• Thawing and handling: Warm sealed aliquots to room temperature before opening to minimize moisture uptake; reseal promptly.
• Stability checks: Periodically verify by LC–MS/HPLC and visual inspection for color change or precipitation. Discard aliquots showing degradation or contamination.

Molecular formula, weight, and exact solubility are not specified for this item; refer to the CoA/Spec Sheet for any lot-specific details.

Structure and Identity

Item-specific (from Product Data)

• SKU: G416718
• Product Name: GI-560192
• CAS: 301326-41-0
• PubChem CID: 6898894
• InChIKey: 281805 (as provided)
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Notes on structural features

• The detailed 2D/3D structure, functional groups, and stereochemistry are not provided in the current product data. Please consult the lot-specific Certificate of Analysis (CoA) or Spectral Pack (NMR/LC–MS/HRMS) for definitive structural confirmation.

Descriptive identity guidance (general)

• GI-560192 is cataloged as a small-molecule research chemical (category: chemical and biochemical reagents). Without a disclosed SMILES or IUPAC name, structural inference is intentionally withheld; end users should authenticate identity via orthogonal methods after receipt.
• Recommended identity verification workflows (general):
  • LC–MS with exact mass confirmation (if MW available on CoA) and retention-time match to supplier reference.
  • 1H/13C NMR in an appropriate deuterated solvent to confirm integrity and exclude major impurities/solvates.
  • Optional: IR and elemental analysis if the empirical formula is supplied.

Synthetic Utility

GI-560192 is listed as a research-use small molecule; it is not presented as a general synthetic reagent or building block in the current product data.

Consequences for synthetic use (not item-specific)

• Without disclosed functional groups or a SMILES, no reactivity, protecting-group strategy, or cross-coupling suitability can be inferred. If derivatization is desired (e.g., to generate analogs for SAR), fully characterize the structure first and identify any reactive handles.
• If the compound contains labile motifs (esters, acylals, diazonium surrogates, etc.), it may be incompatible with strong acids/bases, nucleophiles, or elevated temperatures typically used in synthesis.

Recommendations

• Consult the CoA and any available spectral data to identify functional groups.
• Run small-scale pilot reactions under mild conditions with in-process analytical monitoring (TLC/UPLC–MS) to assess stability before committing material.
• For tagging/immobilization experiments (e.g., biotinylation, photoaffinity), ensure presence of an appropriate attachment point and verify that modifications do not abrogate the intended research performance.

Target Specificity

No target, enzyme/receptor class, or binding epitope information is provided for GI-560192.

• Item-specific data such as target family, species selectivity, and mechanism of action: Not specified for this item; refer to literature associated with CAS 301326-41-0 or PubChem CID 6898894 and any internal data generated by your lab.
• Without validated target specificity, treat GI-560192 as an unannotated chemical entity. Perform appropriate counter-screens and orthogonal assays to define selectivity in your system of interest.