GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human GIT1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function： GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
Specifications
Product Name
GIT1 Antibody - Primary antibody, specific to GIT1, Rabbit IgG
Synonyms
ARF GAP GIT1 | ARF GTPase-activating protein GIT1 | CAT1 | CAT-1 | Cool-associated and tyrosine-phosphorylated protein 1 | G protein-coupled receptor kinase interacting ArfGAP 1 | G protein-coupled receptor kinase interactor 1 | G protein-coupled receptor
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species
Rabbit
Specificity
GIT1
Immunogen
A synthetic peptide derived from human GIT1
Positive Control
WB: A549, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
GIT1 Antibody (Ab105406) - Western Blot All lanes: GIT1 Antibody (Ab105406) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 90 kDa Exposure time: 60.0 s
GIT1 Antibody (Ab105406) - Western Blot All lanes: GIT1 Antibody (Ab105406) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 86 kDa Exposure time: 60.0 s
GIT1 Antibody (Ab105406) - IHC Immunohistochemical analysis of GIT1 Antibody (Ab105406) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the GIT1 Antibody (Ab105406) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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