Grp75 Mouse mAb - Anticuerpo primario, specific to Grp75, IgG2b

Cat. No.: Ab181865

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón

Isotype

Mouse IgG2b

Clone Number

CD02/2E10

Conjugation

Unconjugated

Descripción general

Application

WB: 1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa

ICC/IF: 1/200

Flow: 1/200 - 1/25,000

Especificaciones

Product Name

Grp75 Mouse mAb - Anticuerpo primario, specific to Grp75, IgG2b

Sinónimos

75 kDa glucose regulated protein antibody|75 kDa glucose-regulated protein antibody|CSA antibody|Glucose Regulated Protein antibody|Grp 75 antibody|GRP-75 antibody|GRP75 antibody|Heat shock 70 kDa protein 9 antibody|Heat shock 70kD protein 9 antibody|heat shock 70kDa protein 9 antibody|Heat shock 70kDa protein 9B antibody|Heat shock protein 74 kDa A antibody|Heat shock protein A antibody|Heat shock protein cognate 74 antibody|Hsc74 antibody|Hsp74 antibody|Hsp74a antibody|HSPA9 antibody|Hspa9a antibody|HSPA9B antibody|MGC4500 antibody|mitochondrial antibody|Mortalin 2 antibody|Mortalin antibody|Mortalin perinuclear antibody|Mortalin2 antibody|MOT 2 antibody|MOT antibody|MOT2 antibody|Mthsp70 antibody|p66 mortalin antibody|P66 MOT antibody|PBP74 antibody|Peptide binding protein 74 antibody|Peptide-binding protein 74 antibody|Stress 70 protein mitochondrial antibody|Stress 70 protein mitochondrial precursor antibody|Stress-70 protein antibody|Mouse anti-Human Grp75|Anti-Grp75 mAb|Human Grp75 alpha (HSP70) Antibody|Monoclonal Anti- Grp75 antibody

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL

Especies hospedadoras

Ratón

Especificidad

Grp75

Inmunógeno

Recombinant protein corresponding to Human Grp75

Control positivo

WB: HeLa, HEK293, Jurkat, MCF-7, HepG2, A431, A549, K562, Mouse Brain, NIH/3T3 and C6 Whole cell Lysates Flow: HeLa cells ICC/IF: HeLa cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotipo

IgG2b

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 16.0 s 

Western Blot: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 71 kDa
Exposure time: 2.3 s 

Flow Cytometry: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Grp75 Mouse mAb (Ab181865) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

ICC/IF: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - ICC/IF
IF analysis of Grp75 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Grp75 Mouse mAb (Ab181865) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

Aplicación

Dilution info

IF/ICC

1/200

WB

1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa

Flow

1/200 - 1/25,000

Objetivos asociados (humanos)

HSPA9 Tchem Stress-70 protein, mitochondrial (0 Activities)

Discover Target: HSPA9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Grp75 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.