Hsp60 Mouse mAb - Anticuerpo primario, specific to Hsp60, Mouse IgG1

Cat. No.: Ab169190

CALIDAD & PUREZA Carrier Free, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

EF02/2E10

Conjugation

Unconjugated

Descripción general

Application
WB: 1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
ICC/IF: 1/200
Flow: 1/800 - 1/200,000

Especificaciones

Product Name

Hsp60 Mouse mAb - Anticuerpo primario, specific to Hsp60, Mouse IgG1

Sinónimos

60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib

Especificaciones y pureza

Carrier Free,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Especies hospedadoras

Ratón

Especificidad

Hsp60

Inmunógeno

Recombinant full length protein corresponding to Human Hsp60

Control positivo

WB: HEK293, HeLa, HepG2, A549, MOTL-4, MCF-7, Jurkat, K562, A431 and THP-1 Whole cell Lysates ICC/IF: U-2 OS cells Flow: Jurkat cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, High Performance, PBS Only, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s 

Western Blot: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s 

Flow Cytometry: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

ICC/IF: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

Aplicación

Dilution info

IF/ICC

1/200

Flow Cytometry

1/800 - 1/200,000

WB

1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa

Objetivos asociados (humanos)

HSPD1 Tbio 60 kDa heat shock protein, mitochondrial (0 Activities)

Discover Target: HSPD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HSPD1 Tbio Heat shock protein HSP 60 (26 Activities)

Discover Target: HSPD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp60 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.