Iba1 Mouse mAb - Anticuerpo primario, specific to Human Iba1

Cat. No.: Ab156545

GRADE & PURITY Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Clone Number

AB05/2A5

Conjugation

Unconjugated

Descripción general

Mouse anti Human Iba1 Antibody, clone AB05/2A5, could be used for Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

Iba1 Mouse mAb - Anticuerpo primario, specific to Human Iba1

Sinónimos

AIF 1 antibody | AIF-1 antibody | Aif1 antibody | AIF1 protein antibody | AIF1_HUMAN antibody | Allograft inflammatory factor 1 antibody | Allograft inflammatory factor 1 splice variant G antibody | allograft inflammatory factor-1 splice variant Hara-1 an

Especificaciones y pureza

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the p

Especies hospedadoras

Ratón

Especificidad

Human Iba1

Inmunógeno

Recombinant protein corresponding to Human Iba1

Control positivo

Flow Cytometry: THP-1 cells IF/ICC: U-2 OS cells

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Flow Cytometry: Iba1 Mouse mAb (Ab156545)

Iba1 Mouse mAb (Ab156545) - Flow Cytometry ​
Flow Cytometry analysis of THP-1 cells stained with Iba1  Mouse mAb (Ab156545) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

ICC/IF: Iba1 Mouse mAb (Ab156545)

Iba1 Mouse mAb (Ab156545) - ICC/IF
IF analysis of Iba1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Iba1 Mouse mAb (Ab156545) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

Aplicación

Dilution info

IF/ICC

1/200

Flow Cytometry

1/200 - 1/125000

Objetivos asociados (humanos)

AIF1 Tbio Allograft inflammatory factor 1 (0 Activities)

Discover Target: AIF1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Iba1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.