IGJ Mouse mAb - Anticuerpo primario, specific to IGJ

Cat. No.: Ab109641

GRADE & PURITY Validated, ExactAb™, See COA

Application

IHC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

OTI3H7

Conjugation

Unconjugated

Descripción general

Mouse anti Human IGJ Antibody, Monoclonal (OTI3H7), could be used for WB, IHC and so on.

Specifications

Product Name

IGJ Mouse mAb - Anticuerpo primario, specific to IGJ

Sinónimos

IGCJantibody | IGJ_HUMAN antibody | Immunoglobulin J chain antibody | Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody | immunoglobulin joining chain antibody | JCH antibody | JCHAIN antibody | Joining chai

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to

Especies hospedadoras

Ratón

Especificidad

IGJ

Inmunógeno

Recombinant full length protein of human IGJ.

Control positivo

WB: HEK293T transfected with pCMV6-ENTRY IGJ cDNA for 48 hrs; Human testis, omentum, uterus, breast, brain, ovary, thyroid gland, colon lysates. IHC: Human bladder carcinoma, lymphoma tissues.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - Western Blot
All lanes: IGJ Mouse mAb (Ab109641) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa
Exposure time: 100.0 s

Western Blot: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - Western Blot
All lanes: IGJ Mouse mAb (Ab109641) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/40000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa
Exposure time: 18.0 s

IHC: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using IGJ Mouse mAb (Ab109641). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab109641).

IHC: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using IGJ Mouse mAb (Ab109641). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab109641).

Aplicación

• Aplicación: Dilution info
• WB:

  1/2000;

• IHC:

  1/50

Objetivos asociados (humanos)

JCHAIN Tbio Immunoglobulin J chain (0 Activities)

Discover Target: JCHAIN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IGJ Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.