ITS Plus - Animal Free, sterile-filtered, BioReagent, for cell culture, 100×

Cat. No.: U777559

CALIDAD & PUREZA Animal Free, Sterile-filtered, BioReagent, for Cell culture, 100×

Synonyms

ITSplus | ITS supplement | IGF-1-Transferrin-Selenium Media Supplement

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Descripción general

ITS Plus consists of recombinant human IGF-1, recombinant transferrin, sodium selenite and other components. It contains no phenol red. As a supplement for basal cell culture media, it can reduce the usage of fetal bovine serum during the culture of various cells, and is commonly applied in serum-free or low-serum cell culture.

IGF-1 is a polypeptide hormone with pleiotropic anabolic effects on mammalian cells. It facilitates the uptake of glucose and amino acids, lipogenesis, transport of monovalent cations and phosphates, as well as the synthesis of proteins and nucleic acids. Transferrin acts as an intracellular iron carrier and helps mitigate the toxicity of oxygen free radicals and peroxides. Sodium selenite serves as a cofactor for glutathione peroxidase and other proteins, functioning as an antioxidant in culture media.

Product Features:

1. This product is sterilized by filtration.

2. Free of animal-derived components, it supports low-serum and serum-free cell culture.

3. Applicable to the culture of various cell types and can promote cell growth.

4. Compatible with a wide range of culture media. It effectively boosts cell growth, facilitates primary cell culture, improves the success rate of single-cell cloning and increases protein production.

Precautions:

For research use only. Not intended for clinical diagnosis or treatment. 

Especificaciones

Sinónimos

ITSplus | ITS supplement | IGF-1-Transferrin-Selenium Media Supplement

Especificaciones y pureza

Animal Free, sterile-filtered, BioReagent, for cell culture, 100×

Estabilidad y almacenamiento

Store at -20℃ long term (12 months).

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

Animal Free, BioReagent, Filtrado estéril, para el cultivo celular

Product Line

Productos biológicos

Application Protocols

Item-specific use instructions, concentration, and composition are Not specified for this item; refer to the CoA/Spec Sheet. The following guidance reflects common literature practice for ITS/ITS+ supplements and should be validated empirically:

• Thawing: Store at −20°C (Product Data). Thaw on ice or at 2–8°C. Mix gently by inversion; avoid vigorous vortexing or foaming.
• Aliquoting: Immediately aliquot into single-use portions to avoid repeated freeze–thaw. Keep on ice during aliquoting in a biosafety cabinet.
• Working concentration: Many ITS/ITS+ products are supplied as concentrates (e.g., 100×) and used at 1× final in basal media (literature). Confirm the specific dilution for this item via CoA.
• Medium preparation: Add supplement to pre-warmed (room temperature) or cold basal medium under aseptic conditions. Gently invert to mix. If required, perform sterile filtration (0.2 µm, low-protein-binding).
• Compatibility checks: After supplementation, verify pH and osmolality. Observe for turbidity or precipitation. If combining with growth factors or albumin substitutes, add sequentially and monitor clarity.
• Culture adaptation: When moving from serum-containing conditions, gradually reduce serum while adding the supplement over several passages to support adaptation (literature best practice).
• Storage of prepared medium: Typically 2–8°C for limited periods (days), protected from light; confirm stability for this item via CoA/Spec Sheet.

Biological Roles

Literature context for typical ITS/ITS+ components (not item-specific; composition of this product is Not specified and must be verified by CoA/Spec Sheet):

• Insulin (peptide hormone): Facilitates cellular glucose uptake and metabolism; supports anabolic pathways, promoting survival and proliferation in vitro. Activates PI3K/AKT and MAPK signaling cascades (literature).
• Transferrin (iron transport glycoprotein): Delivers Fe3+ to cells via transferrin receptor–mediated endocytosis, supporting heme synthesis, mitochondrial respiration, and DNA synthesis (literature). Animal-free sources or recombinant forms are commonly used in defined media.
• Selenium (as selenite/selenate): Essential micronutrient incorporated into selenoproteins (e.g., glutathione peroxidases, thioredoxin reductases) that protect against oxidative stress and support redox homeostasis (literature).
• “Plus” components (literature variants): Ethanolamine can serve as a precursor for phosphatidylethanolamine, aiding membrane biosynthesis; some ITS+ blends may include additional defined lipids or factors.

Functional outcome (general): When added to basal media, ITS/ITS+ supplements help sustain proliferation and viability under reduced-serum or serum-free conditions, stabilize metabolic activity, and improve reproducibility by replacing undefined serum factors with defined components.

Note: Verify the exact composition, concentration, and recommended use level for this item via the CoA/Spec Sheet.

Buffer Applications

This product is not a buffering agent per se. However, practical integration with buffered media is important.

• Compatibility: Designed for use in standard cell culture media buffered with bicarbonate/CO2 or HEPES (general, literature). After addition, confirm pH (typically 7.2–7.4 for many mammalian lines) and osmolality to ensure the supplement does not perturb medium conditions.
• Order of addition: Add to cooled, pre-buffered basal medium under aseptic conditions. Mix gently to minimize foaming and protein denaturation. If compounding with other defined additives, verify compatibility and absence of precipitation.
• Filtration: If the final medium must be sterilized, use low-protein-binding 0.2 µm filters. Avoid repeated filtration passes.

Item-specific pH, buffering capacity, and ionic contributions are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

While “green solvent” considerations do not directly apply, the selection of an animal-free, defined supplement can contribute to more sustainable and ethical lab practices.

• Animal Free vs. animal-derived supplements:
  • Potential benefits: Reduces reliance on animal serum, improves batch-to-batch consistency, and can lower environmental and ethical impact associated with animal sourcing (general perspective).
  • Tradeoffs: Some cell lines may require optimization of growth factor and lipid content to match performance of FBS-containing media.

Comparison (general, literature; not item-specific):

• FBS supplementation (10% typical) — robust growth but high variability, animal-origin risks, and significant sourcing footprint.
• ITS/ITS+ supplementation — supports serum-free/low-serum culture with defined components; may require line-specific optimization of concentrations and additional defined additives (e.g., hormones, lipids, albumin substitutes).

For laboratories pursuing greener and more reproducible workflows, transitioning to animal-free, chemically defined media supplemented with ITS-type cocktails is a practical step, provided performance is validated per cell line.

Pharmaceutical Uses

No therapeutic/clinical use is claimed. For research use only (Product Data).

Formulation/manufacturing context (general, literature):

• Animal-free, defined supplements are often evaluated during process development for cell-based production (e.g., recombinant protein or viral vector research) to reduce variability seen with serum. Such evaluation occurs in research and preclinical process development settings.
• Benefits: Lot-to-lot consistency, reduced adventitious agent risk compared with animal serum, and improved analytical characterization of process fluids.
• Considerations: Regulatory-grade (GMP) equivalents may be required for clinical manufacturing; this product is not designated for GMP use. Transitioning from serum-containing to defined systems typically requires cell line–specific optimization.

Item-specific pharmacopeial status, excipient listings, endotoxin limits, and bioburden specifications are Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

As a multi-component biological supplement, discrete single-compound physical constants are not applicable.

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/format (e.g., 100× stock, liquid/frozen): Not specified for this item; refer to CoA/Spec Sheet.
• pH (as supplied): Not specified for this item; refer to CoA/Spec Sheet.
• Osmolality/ionic strength: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Typically fully miscible with aqueous cell culture media (general, literature). Exact solubility/compatibility for this item: refer to CoA/Spec Sheet.
• Density, refractive index, logP, pKa: Not applicable/not defined for a mixture.
• Thermal behavior: Store at −20°C (Product Data). Avoid repeated freeze–thaw to preserve protein integrity (general best practice).
• Light sensitivity: Many protein/supplement components are light-sensitive; minimize light exposure during handling (general guidance).

Literature context (not item-specific): Commercial ITS/ITS+ supplements are commonly provided as sterile-filtered aqueous concentrates (e.g., 100×) intended for 1× final use after dilution into basal media. However, exact formulation, concentration, and stabilizers vary; consult the CoA/Spec Sheet for item-specific properties.

Quality and Grades

• Grade/Purity (Product Data): Animal Free; Sterile-filtered; BioReagent; for Cell culture.
• Animal Free: Manufactured without animal-derived components to minimize adventitious agent risk and batch variability attributable to animal origin materials. This can support regulatory expectations for biologics development and reproducibility in serum-reduced systems (general implications).
• Sterile-filtered: Passed through a sterilizing-grade membrane (typically 0.2 µm) to reduce bioburden. End-user must maintain aseptic technique to preserve sterility.
• BioReagent; for Cell culture: Suitable for research-grade cell culture workflows (not GMP). Low bioburden/particulates; typical QC may include sterility and functional growth-support checks, but item-specific tests are Not specified for this item; refer to CoA/Spec Sheet.
• Appearance and assay: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet.

How to use these grades in practice:

• Choose Animal Free when minimizing animal-origin risks or when transitioning toward chemically defined conditions.
• Use sterile technique end-to-end; consider aliquoting to avoid freeze–thaw cycles.
• For analytical applications (e.g., proteomics-sensitive workflows), verify absence/presence of carrier proteins or additives in the CoA/specification before use.

Reaction and Applications

This product is a biological cell culture supplement, not a chemical reagent for synthetic or analytical reactions.

• Not typically used for organic synthesis, catalysis, or small-molecule reactions.
• For practical use, see Biological Roles and Application Protocols for guidance on supplementing mammalian cell cultures in serum-reduced/serum-free media (general, literature).

If you require a reagent for chemical transformations, consider selecting a pure small-molecule or buffer system tailored to that chemistry domain.

Reaction Conditions

Not applicable. This product is not intended for chemical reactions. There are no recommended temperatures, catalysts, or reaction parameters beyond those relevant to sterile handling and media preparation.

For experimental use, focus on:

• Maintaining asepsis during media supplementation.
• Observing recommended storage at −20°C (Product Data) and minimizing freeze–thaw.
• Verifying cell growth performance through small-scale pilot cultures when adjusting supplement levels (general, literature).

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; consult the SDS for authoritative safety information.
• General hazards (literature/general): Proteinaceous supplements and trace element salts can cause irritation or allergic reactions in sensitized individuals. Handle as a laboratory chemical/biological reagent using appropriate aseptic technique.
• PPE: Lab coat, gloves (nitrile), and safety glasses recommended. Use a certified biological safety cabinet when preparing sterile media to prevent contamination (general best practice).
• Handling: Maintain sterility; work on ice or at 2–8°C during preparation to limit protein degradation. Avoid creating aerosols/splashes. Do not pipette by mouth.
• Incompatibilities: Avoid strong oxidizers and reactive chemicals. Do not mix with media components known to precipitate in the presence of divalent cations or high phosphate without prior compatibility checks (general guidance). Avoid repeated freeze–thaw.
• First aid (general): On skin/eye contact, rinse with water for several minutes. If inhaled, move to fresh air. If ingested, rinse mouth with water. Seek medical attention if symptoms persist. Always defer to SDS.
• Waste: Dispose of according to institutional biosafety and chemical waste procedures. Decontaminate work surfaces after use.

Always consult the SDS and institutional EHS policies for item-specific guidance.

Solvent Selection

This product is intended for aqueous cell culture use rather than as an organic solvent.

• Medium compatibility (general, literature): ITS/ITS+ supplements are typically added to common basal media (e.g., DMEM, RPMI, Ham’s F-12) and serum-free or low-serum formulations. They are fully miscible with water-based media.
• Ionic/serum considerations: Divalent cations, high phosphate, or certain buffer systems can affect protein stability or solubility. When formulating custom media, test for precipitation and verify pH/osmolality post-addition (general guidance).
• Filtration: If compounded into custom media, final solution is commonly sterilized by 0.2 µm filtration under aseptic conditions. Avoid filtration of highly proteinaceous mixes through small-volume syringe filters that can exhibit significant protein binding; use low-protein-binding membrane types (general guidance).
• Alternative carriers: For lipid-containing variants (literature), ensure compatibility with emulsifiers or albumin substitutes used in animal-free systems.

Item-specific vehicle, concentration, and excipients are Not specified for this item; refer to CoA/Spec Sheet for definitive solvent/media compatibility notes.

Storage and Reconstitution

• Storage temperature: Store at −20°C (Product Data). Shipments are made in an ice chest with ice pads (Product Data). Minimize time at ambient temperature upon receipt.
• Light and temperature: Protect from light. Avoid prolonged exposure to room temperature. Do not store in frost-free freezers that cycle above −20°C.
• Freeze–thaw: Avoid repeated freeze–thaw cycles. Aliquot into sterile, single-use portions upon first thaw (general best practice).
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If supplied lyophilized (unknown), reconstitute with sterile water or buffer per the CoA, mix gently until fully dissolved, and sterile-filter if instructed. If supplied as a liquid concentrate, dilute to working strength with sterile basal medium under aseptic conditions (general, literature).
• Shelf life/stability: Not specified for this item; refer to CoA/Spec Sheet. For prepared media, typical in-use stability at 2–8°C is limited (days) and varies with composition (literature); prepare fresh as feasible.
• Handling: Use aseptic technique in a certified biosafety cabinet. Label aliquots with lot number, concentration, and preparation date to maintain traceability.

Always consult the product CoA/Spec Sheet and SDS for authoritative instructions and stability information specific to this SKU.

Structure and Identity

This product is a defined, multi-component cell culture supplement commonly known as “ITS Plus,” intended to support serum-reduced or serum-free growth conditions. It is not a single, discrete molecule; therefore, small-molecule identifiers do not apply.

• Item nature: Multi-component supplement (mixture); animal-free; sterile-filtered; BioReagent grade for cell culture (Product Data)
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not applicable to a mixture; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable to a mixture; Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChI / InChIKey: Not applicable to a mixture.
• Structural features (general, literature): Typical “ITS” supplements include insulin (peptide hormone), transferrin (iron-transport glycoprotein), and selenium (as a small-molecule selenite/selenate salt); “ITS+” or “ITS Plus” formulations in the literature may also contain ethanolamine and/or other lipid precursors. Exact species, concentrations, and stabilizers vary by supplier and lot; consult the CoA for this item.
• 2D description (general): Aqueous solution or reconstituted mixture of macromolecular proteins (insulin, transferrin) and small inorganic/organic micronutrients (selenium salt; optionally ethanolamine), designed to complement basal media.

Important: Composition and concentrations are not specified for this item; refer to the CoA/Spec Sheet for definitive identity details.

Synthetic Utility

Not applicable. This is a biological supplement for cell culture and is not used as a building block or reagent in chemical synthesis.

For laboratory planning, please see Biological Roles, Application Protocols, and Storage & Reconstitution sections for guidance relevant to this product type.

Target Specificity

Not applicable. This item is not an antibody, probe, or targeted binding reagent. No antigen/epitope specificity, clone, isotype, or species reactivity applies.