Serum Preparation Experiment

Summary

Antisera are sera containing a certain class of antibody molecules with specific immune functions, generally prepared from animals that have been artificially injected with a certain type of antigen. Highly effective antisera are used in research as well as in the diagnosis and treatment of disease.

Operation method

Serum Preparation Experiment

Materials and Instruments

Blood
Cryogenic centrifuge 4 ℃ refrigerator Test tubes -80 ℃ low temperature storage box

Move

1. After blood collection, allow blood to clot for 1 to 2 hours at 37°C (without anticoagulant);
2. Refrigerate at 4°C overnight (to allow the clot to solidify);
3. When the serum precipitates naturally, centrifuge the serum at 3000 rpm for 10 minutes at 4°C. Separate the serum and discard the insoluble material;
4. Transfer the serum to a clean test tube and divide into small portions for storage at -80°C.

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