Recombinant Human C8orf48 Protein: FAQs & Key Facts
Recombinant Human C8orf48 Protein: FAQs & Key Facts
Recombinant Human C8orf48 Protein is a research-grade recombinant protein derived from the human chromosome 8 open reading frame 48 gene, produced for use in biochemical assays, functional studies, and antibody validation workflows.
- Product Name
- Recombinant Human C8orf48 Protein
- SKU
- rp376076
- Gene / Target
- C8orf48 (Chromosome 8 Open Reading Frame 48)
- Species Origin
- Human (Homo sapiens)
- Format
- Recombinant protein; specific formulation details should be confirmed in the product datasheet
- Recommended Storage
- Refer to product datasheet for validated storage conditions and stability data
- Supplier
- Aladdin Scientific
What Is C8orf48 and Why Is It Studied?
C8orf48 (Chromosome 8 Open Reading Frame 48) is a human gene located on chromosome 8. As with many open reading frame genes, detailed functional characterization of C8orf48 is an active area of investigation in the research community. Recombinant human C8orf48 protein enables researchers to study the molecular function, protein–protein interactions, and potential biological roles of this gene product in both normal physiology and disease contexts.
Access to a purified, recombinant form of the protein is essential for generating reliable experimental data, including use in binding assays, enzyme activity studies (if applicable), and as an antigen for antibody production or validation.
Product Specifications and Quality
Recombinant Human C8orf48 Protein (SKU: rp376076) supplied by Aladdin Scientific is intended for research use. For precise specification details — including expression host system, protein tag, molecular weight, purity grade, endotoxin levels, and formulation buffer — researchers should consult the official product datasheet available on the Aladdin Scientific website or request it from technical support.
When evaluating recombinant proteins, key quality attributes to confirm include:
- Purity: Typically assessed by SDS-PAGE and/or HPLC; purity thresholds vary by intended application.
- Endotoxin levels: Critical for cell-based assays; confirm LAL assay results in the datasheet.
- Biological activity: Confirm whether functional activity data is available for this product.
Handling, Reconstitution, and Storage
Proper handling of recombinant proteins is critical to maintaining their integrity and activity. General best practices applicable to Recombinant Human C8orf48 Protein include:
- Reconstitution: If supplied lyophilized, reconstitute in the buffer specified in the product datasheet. Allow the vial to equilibrate to room temperature before opening to avoid condensation.
- Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
- Storage: Follow the specific storage temperature and stability guidelines provided in the product datasheet. Recombinant proteins are typically stored at –20 °C or –80 °C depending on formulation.
- Avoid repeated freeze–thaw: Each cycle can degrade protein quality; use stabilizing additives such as carrier proteins if required for long-term stability.
Always refer to the product-specific datasheet for validated handling and storage conditions for rp376076.
Common Research Applications
Recombinant Human C8orf48 Protein is suitable for a range of in vitro research applications. Typical uses for recombinant proteins of this class include:
- ELISA and immunoassay development: As a standard antigen for quantitative assay calibration.
- Antibody production and validation: As an immunogen for raising antibodies or as a positive control antigen to validate antibody specificity.
- Protein interaction studies: Co-immunoprecipitation (Co-IP), pull-down assays, or surface plasmon resonance (SPR) to identify binding partners.
- SDS-PAGE / Western blot controls: As a molecular weight marker or positive control.
- Structural studies: Depending on purity and yield, may be suitable for biophysical characterization techniques.
Researchers should validate the suitability of rp376076 for their specific assay format prior to use in critical experiments.
Ordering, Availability, and Technical Support
Recombinant Human C8orf48 Protein is available from Aladdin Scientific under SKU rp376076. To place an order or check current stock availability, visit the Aladdin Scientific website or contact your regional sales representative.
For technical questions regarding reconstitution protocols, recommended assay conditions, or compatibility with specific experimental formats, please contact Aladdin Scientific's technical support team. The team can also provide the most current product datasheet and certificate of analysis (CoA) for any given lot.
For questions about shipping, storage during transit, payment options, returns, or account management, refer to the relevant sections of the Aladdin Scientific website or contact customer service directly.
Frequently asked questions
What is C8orf48 and what does the recombinant protein offer for research?
C8orf48 (Chromosome 8 Open Reading Frame 48) is a human gene whose protein product is under active functional investigation. The recombinant human C8orf48 protein (rp376076) provides researchers with a purified, defined protein substrate for studying its molecular interactions, validating antibodies, or developing immunoassays targeting this protein.
What expression system is used to produce Recombinant Human C8orf48 Protein (rp376076)?
The specific expression host system (e.g., E. coli, baculovirus/insect cell, mammalian, or yeast) for rp376076 should be confirmed in the product datasheet provided by Aladdin Scientific. Choosing the appropriate expression system is important for downstream applications where post-translational modifications may be required.
How should I store Recombinant Human C8orf48 Protein to maintain its activity?
General best practice for recombinant proteins is to aliquot the protein upon reconstitution, store at –20 °C or –80 °C as specified in the product datasheet, and avoid repeated freeze–thaw cycles. Always consult the rp376076 product datasheet for lot-specific validated storage conditions and shelf life.
Can Recombinant Human C8orf48 Protein be used for antibody validation?
Yes, recombinant proteins are commonly used as positive control antigens in Western blotting, ELISA, and immunoprecipitation workflows to confirm antibody specificity and sensitivity. Confirm that rp376076 carries the relevant epitope region before use and consult the datasheet for purity and formulation details.
How do I place an order for rp376076 and what support is available?
Recombinant Human C8orf48 Protein (rp376076) can be ordered directly through the Aladdin Scientific website or via your regional sales contact. For technical guidance, certificate of analysis requests, or questions about compatibility with your specific experimental application, Aladdin Scientific's technical support team is available to assist.
References
- Aladdin Scientific product page: Recombinant Human C8orf48 Protein, SKU rp376076.
- NCBI Gene entry for C8orf48 (Homo sapiens). National Center for Biotechnology Information.
- General principles of recombinant protein expression and quality assessment — textbook reference. Specific edition and authors to be verified by editorial team.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
