Recombinant Human GPATCH4 Protein: Overview & Research Use

Recombinant Human GPATCH4 Protein is a recombinantly expressed form of the human G-patch domain-containing protein 4, a nuclear factor implicated in RNA processing and the regulation of RNA helicase activity.

Protein Name

Recombinant Human GPATCH4 Protein

SKU

rp375064

Protein Family

G-patch domain-containing proteins

Species Origin

Human (Homo sapiens)

Primary Function

Putative RNA helicase cofactor involved in RNA processing

Cellular Localization

Nucleus

Format

Recombinant protein (details: see product datasheet)

Biological Background of GPATCH4

GPATCH4 (G-patch domain-containing protein 4) is a member of the G-patch domain protein superfamily, characterized by a conserved glycine-rich motif of approximately 45–50 amino acids. G-patch domains are found across eukaryotes and are known to interact with and stimulate the ATPase and helicase activities of DEAH/RHA-family RNA helicases, thereby playing important regulatory roles in pre-mRNA splicing, ribosome biogenesis, and other RNA metabolic processes.

GPATCH4 is predominantly localized to the nucleus, consistent with a role in nuclear RNA processing events. While its precise molecular functions are still under active investigation, sequence analysis and domain architecture suggest that GPATCH4 may function as a cofactor that recruits or activates specific RNA helicases at defined steps in RNA maturation pathways.

Biochemical Properties & Product Specifications

Recombinant Human GPATCH4 Protein (SKU: rp375064) is produced using recombinant expression technology to ensure consistent quality and batch-to-batch reproducibility. The protein retains the characteristic G-patch domain and is intended for use in biochemical and cell biology research applications.

Key biochemical attributes of GPATCH4 include its glycine-rich G-patch motif, which mediates protein–protein interactions with RNA helicase partners, and its nuclear localization, which reflects its role in nuclear RNA biology. Researchers should consult the official product datasheet for confirmed details on host expression system, tag information, purity grade, molecular weight, buffer composition, and recommended storage conditions, as these specifications are subject to lot-specific variation.

Note: Specific numerical values for purity, concentration, and endotoxin levels are not reproduced here to avoid inaccuracy; please refer to the current product datasheet (SKU: rp375064) or contact Aladdin Scientific technical support.

Research Applications

Recombinant Human GPATCH4 Protein is suitable for a range of in vitro and biochemical research applications. Primary areas of use include:

• Protein–protein interaction studies: GPATCH4 can be used in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance to characterize its interactions with DEAH-box and other RNA helicase family members.
• RNA helicase activity assays: As a putative helicase cofactor, recombinant GPATCH4 can be evaluated for its ability to stimulate the ATPase or unwinding activity of partner helicases in biochemical reconstitution experiments.
• Structural biology: The purified protein may serve as a component for crystallography, cryo-electron microscopy, or NMR studies aimed at resolving the structure of the G-patch domain and its complexes.
• Antibody development & validation: Recombinant GPATCH4 can serve as an immunogen or positive control antigen for antibody production and validation workflows.
• Functional genomics support: Used alongside knockdown or knockout cellular models to perform rescue experiments and confirm on-target phenotypes.

Researchers should validate the protein's performance in their specific assay system prior to use in critical experiments.

Handling, Storage & Quality Considerations

Proper handling and storage of recombinant proteins are essential for maintaining activity and preventing degradation. General best practices for Recombinant Human GPATCH4 Protein include:

• Storage temperature: Store at –80 °C for long-term storage. Avoid repeated freeze–thaw cycles, which can reduce protein integrity. Aliquot upon receipt if repeated access is anticipated.
• Reconstitution: If supplied lyophilized, reconstitute according to the product datasheet using the recommended buffer. Allow the vial to equilibrate at room temperature briefly before opening.
• Handling: Work on ice where possible. Use low-protein-binding tubes and pipette tips to minimize adsorption losses.
• Quality control: Each lot is subject to quality control testing; refer to the certificate of analysis (CoA) provided with your shipment for lot-specific data including purity by SDS-PAGE and biological activity where applicable.

For application-specific buffer requirements or compatibility questions, consult Aladdin Scientific technical support.

Frequently asked questions

What is GPATCH4 and what is its biological function?

GPATCH4 is a G-patch domain-containing nuclear protein found in humans. It belongs to a family of proteins that use a conserved glycine-rich motif to interact with and modulate the activity of DEAH/RHA-family RNA helicases. GPATCH4 is thought to act as a cofactor in RNA processing pathways, although its precise in vivo substrates and regulatory mechanisms remain under investigation.

What expression system is used to produce Recombinant Human GPATCH4 Protein (rp375064)?

The specific expression system, tag configuration, and host organism for SKU rp375064 are detailed in the official product datasheet available from Aladdin Scientific. Researchers are encouraged to download the current datasheet or contact technical support for the most up-to-date lot-specific information before placing an order.

What applications is Recombinant Human GPATCH4 Protein validated for?

Recombinant Human GPATCH4 Protein is suitable for applications including protein–protein interaction studies (e.g., pull-down, co-IP), biochemical helicase activity assays, structural biology studies, and antibody development or validation. Users should confirm suitability for their specific assay conditions and validate performance in their own experimental system prior to critical use.

How should I store Recombinant Human GPATCH4 Protein to maintain its activity?

Store Recombinant Human GPATCH4 Protein at –80 °C for long-term stability. Avoid repeated freeze–thaw cycles by aliquoting the protein upon receipt. Always refer to the product-specific datasheet and certificate of analysis for storage buffer details and any special handling instructions applicable to the current lot.

Can Recombinant Human GPATCH4 Protein be used for structural biology applications such as cryo-EM or X-ray crystallography?

Recombinant Human GPATCH4 Protein may be suitable for structural biology applications, including cryo-EM or X-ray crystallography, particularly for studies of the G-patch domain or its interaction with RNA helicase partners. However, suitability depends on achieved purity, homogeneity, and conformational integrity; researchers should assess these parameters and consult the product datasheet for lot-specific purity data.

References

1. Studer MK, Ivanović L, Weber ME, Marti S, Jonas S. Structural basis for DEAH-helicase activation by G-patch proteins. Proc Natl Acad Sci USA. 2020;117(13):7159-7170. doi:10.1073/pnas.1913880117
2. Bohnsack KE, Bohnsack MT. Uncovering the assembly pathway of human ribosomes and its emerging links to disease. EMBO J. 2019;38(13):e100278. doi:10.15252/embj.2018100278
3. Robert-Paganin J, Réty S, Leulliot N. Regulation of DEAH/RHA helicases by G-patch proteins. Biomed Res Int. 2015;2015:931857. doi:10.1155/2015/931857

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.