KDM5-C70 - 10mM in DMSO , CAS No.1596348-32-1

CAS: 1596348-32-1 Cat. No.: K656700 Fórmula: C₁₇H₂₈N₄O₃ Peso molecular: 336.43 PubChem CID: 90094283

GRADE & PURITY 10mM in DMSO

Storage

Argon charged,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

KDM5-C70 is an ethyl ester derivative of KDM5-C49 and a potent, cell-permeable and pan- KDM5 histone demethylase inhibitor. KDM5-C70 has an antiproliferative effect in myeloma cells, leading to genome-wide elevation of H3K4me3 levels

In Vitro

KDM5-C70 (10 -9 -10 -5 M; 7 days; MM.1S myeloma cells) treatment shows antiproliferative effects after 7 days of treatment at elevated concentrations (estimated 50% reduction of viability/proliferation for KDM5-C70 at ~20 μM). KDM5-C70 (50 μM; 7 days; MM.1S myeloma cells) treatment decreases the level of phosphorylation of retinoblastoma protein (Rb), while leaving the total level of phosphorylated Rb (pRb) unchanged, indicating impairment of cell cycle progression. Chromatin immunoprecipitation followed by next-generation sequencing shows an increase in H3K4me3 levels around transcription start sites with KDM5-C70 but little change with GSK467A at 50 μM inhibitor concentrations. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: MM.1S myeloma cells Concentration: 10 -9 -10 -5 M Incubation Time: 7 days Result: Showed antiproliferative effects after 7 days of treatment at elevated concentrations. Western Blot AnalysisCell Line: MM.1S myeloma cells Concentration: 50 μM Incubation Time: 7 days Result: Decreased the level of phosphorylation of retinoblastoma protein (Rb).

IC50& Target:KDM5 histone demethylase

Specifications

Especificaciones y pureza

10mM in DMSO

Mecanismos bioquímicos y fisiológicos

KDM5-C70 is an ethyl ester derivative of KDM5-C49 and a potent, cell-permeable and pan- KDM5 histone demethylase inhibitor. KDM5-C70 has an antiproliferative effect in myeloma cells, leading to genome-wide elevation of H3K4me3 levels.

Condiciones de almacenamiento de almacenamiento

Argon charged,Store at -80°C

Enviado en

Dry ice packs + Cold packs

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Tipo de acción

INHIBITOR

Nombres e identificadores

• Isómeros SMILES: CCN(CCN(C)C)C(=O)CNCC1=NC=CC(=C1)C(=O)OCC
• PubChem CID: 90094283
• Peso molecular: 336.43