Is it sterile: Yes
Metallozyme is a water-soluble protein using recombinant genes, lyophilized and irradiated with cobalt-60, and aseptic packaging of celine bottles. A recombinant gene protein constructed according to the chemical structure of cephalosporins and penan. The active center of its gene fragment can destroy the chemical structural formula of cephalosporins and penan antibiotics, so that the antibacterial properties of the two drugs are lost after ring opening/chain breaking.
When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly.
Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane.
During sterility test, take 1 bottle of freeze-dried metal enzyme powder and add 5ml of sterile water to dissolve, shake well to make a metal enzyme solution, take 2ml of enzyme solution and put 1500ml of washing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. A manual syringe was used to Pierce the respiratory mouth of the three incubators, and 1ml of enzyme solution was added to each of them, and the enzymes were spread on the entire surface of the filter membrane as far as possible. Then, the high concentration of enzymes was fully in contact with the filter membrane of the incubator, so as to destroy (neutralize and inactivate) the residual cephalosporins and penem drugs on the filter membrane, and then pumped into the corresponding medium and shook well. Positive pairs were treated with 1ml of E. coli (100CFU/ml).
Adding 1ml of the metal enzyme solution to the three incubators can remove the residual cephalosporins and penem on the inner wall of the incubators and the surface of the filter membrane.
Shake the positive pair gently once a day in the morning and afternoon.
Customers can do methodological verification according to the above, but also according to the actual operation of the verification.
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