Lactose Broth (USP) - BioReagent

Cat. No.: L1528500

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Descripción general

For coliform determination. Preparation method: Weigh 13.0 g of this product in 1 L of distilled water, heat to boiling until completely dissolved, autoclave at 121°C for 15 minutes, cool rapidly after sterilization, and set aside for use.

Specifications

Especificaciones y pureza

BioReagent

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Grado

BioReagent

Product Line

Productos biológicos

Application Protocols

Item‑specific, validated protocols are Not specified for this item; refer to CoA/Spec Sheet and your governing method. The following is general literature guidance for a common use case—presumptive lactose fermentation testing:

• Medium preparation: Weigh dehydrated Lactose Broth; add to purified water to the method‑specified concentration. Heat with stirring to dissolve. Dispense into tubes containing inverted Durham vials if gas detection is required. Autoclave per validated cycle; cool to room temperature without trapping air.
• Inoculation: Add the specified inoculum or sample volume aseptically. Include a positive control (e.g., known lactose‑fermenter) and a negative control (non‑fermenter or sterile water).
• Incubation: Incubate at the temperature/time defined by your method (literature examples: 30–37 °C, 24–48 h).
• Readout: Observe for turbidity (growth), gas in Durham tube, and acid (if pH indicator is present in your method). Record results and proceed to confirmatory tests on selective/differential media as required.
• Quality records: Document lot number, preparation date, sterilization parameters, and control results for audit compliance.

Research Use Only: As per Product Data, this product is for research use only.

Biological Roles

While the product itself is a culture medium, its biological relevance centers on bacterial lactose metabolism and growth support.

• Lactose utilization (literature): Many Enterobacteriaceae and other bacteria ferment lactose via beta‑galactosidase (LacZ), cleaving lactose to glucose and galactose, which feed into glycolysis and the Leloir pathway. Acid and/or gas production from this metabolism is the basis for presumptive tests.
• Growth support: Complex nutrients (e.g., peptones, beef extract—literature) provide amino acids, peptides, vitamins, and trace minerals to sustain a broad range of non‑fastidious microbes.
• Induction: Presence of lactose can induce the lac operon in organisms possessing the regulatory machinery, affecting expression of transport (LacY) and catabolic enzymes.
• Selectivity: Lactose Broth is generally non‑selective; it does not inhibit non‑target organisms, making it suitable for enrichment but necessitating confirmatory selective steps downstream.
• QC organisms (examples, literature): Escherichia coli and Enterobacter aerogenes are frequently used as positive controls for lactose fermentation; Pseudomonas aeruginosa or Proteus spp. may serve as negative/weak fermenters depending on the method. Specific control strains for this item are Not specified; refer to your method.

Note: Use of this medium is strictly for research or laboratory quality control. It is not intended for clinical diagnosis or therapeutic applications.

Buffer Applications

Lactose Broth (USP) is a nutrient culture medium, not a buffering reagent. It does not function as a defined buffer system with a specific pKa/pH window.

• If pH control is critical, utilize dedicated buffer systems (e.g., phosphate, HEPES, MOPS) or select media with defined buffering capacity appropriate for your organism and assay.
• Prepared Lactose Broth typically exhibits a near‑neutral pH (literature), adequate for many enteric bacteria during short incubations, but its buffering capacity is modest and composition‑dependent.

For electrophoresis, chromatography, or biochemical buffers, refer to dedicated buffering reagents rather than this complex medium.

Green Alternatives

For culture media, “green” considerations focus on sourcing, waste, and energy, rather than solvent replacement.

• Energy and sterilization: Optimize autoclave loading and cycle efficiency to reduce energy and water consumption. Where validated, terminal sterile filtration of small volumes can reduce autoclave runs.
• Sourcing: Consider media that use plant‑derived peptones to reduce animal‑origin inputs when acceptable for your workflow (check compendial compatibility first).
• Waste minimization: Prepare only the volume needed; store aliquots appropriately to extend shelf life and reduce discards. Deactivate spent cultures efficiently (e.g., consolidated autoclave cycles) to cut consumable use.
• Ready‑to‑use vs dehydrated: Dehydrated media (like this product) generally reduce shipping mass/volume and container waste compared with bottled ready‑to‑use broths.

Comparison (general literature guidance):

• Lactose Broth vs Lauryl Tryptose Broth (LTB): LTB contains selective agents (e.g., sodium lauryl sulfate) that can reduce false positives but may add environmental load if disposed in bulk. Lactose Broth is non‑selective, often yielding higher sensitivity in presumptive steps with potentially simpler waste handling.
• Powdered media vs prepared plates/broth: Powdered formats reduce single‑use plastics and transport emissions; prepared formats save in‑lab energy but generate more packaging waste.

Always balance sustainability with method compliance and data quality.

Pharmaceutical Uses

No therapeutic or clinical use is claimed. In pharmaceutical and related regulated environments, Lactose Broth (USP) is relevant as a laboratory medium for microbiological quality control (QC) testing.

• Role (literature): Used in compendial microbiological tests as a presumptive enrichment or detection medium for lactose‑fermenting organisms in non‑sterile product testing, water systems, and environmental monitoring workflows, depending on the method.
• Regulatory context: “USP” in the name denotes alignment with pharmacopeial applications. Users are responsible for verifying method suitability and growth promotion with their products and organisms of interest per USP chapters (e.g., microbiological examination of non‑sterile products). This item is labeled For research use only; it is not manufactured under GMP unless explicitly stated elsewhere.
• Formulation/excipient status: Not an excipient; not intended for incorporation into drug products.
• Documentation: Maintain CoA, SDS, and internal qualification records (growth promotion, negative controls) with each lot for audit trails.

Item‑specific compendial references, organism panels, and performance ranges: Not specified for this item; refer to CoA/Spec Sheet and your governing pharmacopeia/method.

Physical Properties

• Physical state (as supplied): Typically a dry, free‑flowing powder blend; appearance for this specific item: Not specified for this item; refer to CoA/Spec Sheet.
• Color/odor: Literature—light tan to beige, mild characteristic aroma of complex peptones. Item‑specific attributes: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Water‑soluble; formulated to dissolve/disperse in purified water at the method‑specified concentration (literature: commonly on the order of tens of grams per liter). Exact use concentration for this item: Not specified for this item; refer to CoA/Spec Sheet.
• pH (prepared medium): Literature—USP lactose broth is typically near neutral pH after sterilization (approx. pH 6.5–7.5). Item‑specific pH: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling/melting point, density, refractive index, logP, pKa: Not applicable to a complex solid mixture or not typically reported; Not specified for this item; refer to CoA/Spec Sheet.
• Osmolality/ionic strength (prepared medium): Method‑dependent; Not specified for this item; refer to CoA/Spec Sheet.

Preparation behavior (literature): The broth usually clarifies upon heating; minor opalescence can remain due to complex nutrients. Autoclaving commonly used (e.g., 121 °C, 15 min), but follow your validated method.

Note: Because this is a formulated medium, most physicochemical constants are not represented by single values; consult the product label/CoA for target pH and recommended preparation parameters.

Quality and Grades

• Item grade: BioReagent. This indicates suitability for biological laboratory workflows. It emphasizes consistent performance and low levels of inhibitory contaminants for microbial culture applications, but is not equivalent to GMP unless specifically stated.
• “USP” designation: In the context of culture media, “USP” refers to alignment with United States Pharmacopeia applications (e.g., microbiological quality tests). It typically implies the formulation/performance supports compendial methods. Always confirm compatibility with the version of the USP you follow and consult the product CoA for lot‑specific performance criteria (e.g., growth promotion tests), if available.
• Lot-to-lot consistency: Critical for QC labs. For this item, specific acceptance ranges (pH of prepared medium, sterility, growth promotion) are Not specified for this item; refer to CoA/Spec Sheet.
• Additives/stabilizers: Not specified for this item; refer to CoA/Spec Sheet. In general, properly blended dehydrated media are stable under dry, room‑temperature storage.
• Suitability: Appropriate for research use (Research Use Only as noted). If using for regulated QC testing, ensure your internal qualification confirms performance with relevant compendial organisms and conditions.

Practical tip: Retain a sample of each lot for internal verification. Document growth promotion (positive/negative controls) upon receipt and prior to use in critical assays.

Reaction and Applications

This product is used for microbiological culture, not for chemical synthesis. Key laboratory applications include:

• Presumptive detection of lactose‑fermenting bacteria by monitoring acid and/or gas formation from lactose.
• Quality control testing in food, water, cosmetics, and pharmaceutical environments aligned with compendial methods that begin with non‑selective lactose fermentation screening.
• Pre‑enrichment or presumptive test before confirmatory/confirmed tests using selective/differential media.
• Use with Durham tubes (literature) to capture gas as an indicator of fermentation; incubate at method‑defined temperatures and times.
• Educational and research workflows exploring carbohydrate utilization, beta‑galactosidase induction, and metabolic profiling.

Practical tips (literature/general):

• Prepare the broth with high‑purity water; mix thoroughly to fully dissolve. Distribute into tubes with Durham vials if gas detection is required. Autoclave per validated cycle (e.g., 121 °C) and cool without introducing air bubbles into Durham tubes.
• Inoculate with a defined volume of sample or culture as directed by the method (volume typically scaled to vessel size). Incubate under aerobic conditions unless otherwise specified.
• Always include positive and negative controls to verify medium performance and interpretive criteria. For regulatory testing, document growth‑promotion suitability for the organisms of interest.

Item‑specific directions for use: Not specified for this item; refer to CoA/Spec Sheet and your governing method.

Reaction Conditions

This section is interpreted as preparation and incubation conditions for microbiological use, not chemical reactions.

• Reconstitution (general literature): Dissolve the dehydrated medium in purified water at the method‑specified concentration. Item‑specific concentration and target pH: Not specified for this item; refer to CoA/Spec Sheet.
• Sterilization: Typically autoclave at 121 °C for ~15 minutes for standard volumes, unless your validated method dictates otherwise. Avoid overheating that can cause Maillard reactions or caramelization affecting performance.
• Dispensing: For gas detection, place Durham tubes inverted in culture tubes before dispensing. Fill to cover Durham tube fully; cap loosely for autoclaving to avoid collapse, then tighten aseptically after cooling.
• Incubation (literature): Presumptive tests often incubate at 30–37 °C for 24–48 h, monitoring for acid (pH drop/indicator in some protocols) and/or gas production. Exact temperatures, durations, and interpretation criteria must follow your chosen standard (USP, AOAC, etc.).
• Controls: Include appropriate positive/negative control strains to verify growth promotion and selectivity (if any). Document results before testing samples.

Item‑specific time/temperature parameters and acceptance criteria: Not specified for this item; refer to CoA/Spec Sheet and your validated SOP.

Safety and Handling

• GHS classification, signal word, pictograms, H‑statements: Not specified for this item; refer to the product SDS for authoritative information.
• General hazards (literature/good practice): Culture media powders can cause dust irritation to eyes, skin, and respiratory tract. Avoid inhalation and contact. Not known to be acutely toxic at use concentrations, but can support microbial growth—treat used broth as biohazardous material per institutional procedures.
• PPE: Wear lab coat, nitrile gloves, and safety glasses/goggles. Use a dust mask or respirator if dust generation cannot be controlled (per risk assessment/SDS).
• Handling: Minimize dust. Weigh in a clean, dry area. Use clean glassware and high‑purity water to prevent contamination. Sterilize per your validated protocol before microbiological use.
• Incompatibilities: Store away from moisture and strong oxidizers. Avoid prolonged exposure to heat and light that may degrade growth factors.
• First aid (overview; defer to SDS): If inhaled, move to fresh air. In case of skin/eye contact, rinse with water. If ingested, rinse mouth; seek medical advice as needed. For exposure to cultures grown in the broth, follow biosafety procedures.
• Waste: Autoclave or chemically disinfect spent cultures before disposal in accordance with local regulations and institutional biosafety policies.
• Shipping/storage: Shipped under normal conditions; store at room temperature as provided in the Product Data. Keep container tightly closed and dry.

Solvent Selection

As a microbiological broth medium, the intended solvent is purified water. Organic solvent selection is not applicable.

• Solvent: Use sterile, deionized/distilled water that meets your laboratory or pharmacopeial requirements (e.g., Purified Water per USP). High ionic contaminants or residual sanitizers can inhibit growth.
• Miscibility: Fully soluble/dispersible in water at the method‑specified concentration (item‑specific concentration Not specified for this item; refer to CoA/Spec Sheet).
• Preparation considerations (literature): Dissolve the dehydrated medium with gentle heat and stirring. Adjust final volume at temperature per method. Sterilize (commonly by autoclaving) if required by your application; some labs sterile‑filter heat‑labile supplements added post‑autoclave.
• When to choose Lactose Broth vs alternatives:
  • Choose Lactose Broth for presumptive detection of lactose‑fermenting bacteria (e.g., coliform screening with Durham tubes).
  • Consider alternatives (e.g., Lauryl Tryptose Broth, MacConkey Broth) when selective agents or differential indicators are needed to suppress non‑targets or visualize acid production.

Note: Water quality and container cleanliness frequently dominate performance; verify that carboys and dispensers are free of residual detergents or disinfectants.

Storage and Reconstitution

• Storage (as supplied): Store at room temperature in a tightly closed container, protected from moisture and excessive heat/light. Shipped under normal conditions (per Product Data).
• Shelf life: Item‑specific expiration and retest dates are Not specified for this item; refer to CoA/Spec Sheet and package label.
• Reconstitution (general literature guidance):
  • Use purified water. Add the dehydrated medium to water while stirring; warm gently to aid dissolution.
  • Adjust final volume at temperature as per method. Target pH after sterilization is method‑defined; item‑specific pH Not specified for this item; refer to CoA/Spec Sheet.
  • Sterilize by autoclaving if required. Do not over‑autoclave to avoid nutrient degradation.
  • For supplements (if applicable), sterilize separately by filtration and add aseptically after the base medium cools to ~45–50 °C.
• Prepared medium storage: If not used immediately, store prepared broth at 2–8 °C (literature) for a limited period to maintain performance; duration Not specified for this item—establish per your lab’s QC.
• Freeze–thaw: Avoid freezing prepared complex media unless validated, as precipitation and performance changes can occur.

Always consult the CoA/Spec Sheet and your validated SOP for item‑specific preparation ratios, pH targets, and hold times.

Structure and Identity

Lactose Broth (USP) is a microbiological culture medium, not a single chemical substance. It is a formulated mixture intended for cultivation and detection of lactose‑fermenting microorganisms.

• Item type: Complex culture medium (mixture); individual compound identifiers (CAS, InChIKey, SMILES) are not applicable to the complete medium.
• SKU: L1528500; Product Name: Lactose Broth (USP); Grade: BioReagent.
• Molecular formula and molecular weight: Not specified for this item; refer to CoA/Spec Sheet. (Not applicable to mixtures.)
• Structural features (descriptive): The medium typically contains an energy/carbon source (lactose) plus nitrogen sources and growth factors (e.g., peptone/tryptone, beef extract) and mineral content, providing nutrients for bacterial growth. As a broth, it is supplied as a homogeneous powder blend; when dissolved in water and sterilized, it forms a clear to slightly opalescent nutrient solution.
• Identity standard: “USP” in the name denotes alignment with United States Pharmacopeia compendial use for specified microbiological tests; specific recipe and performance criteria should be confirmed against the current USP chapter and the product’s CoA.

General (literature) note: USP Lactose Broth is classically used for presumptive coliform testing by monitoring acid and/or gas from lactose fermentation. Exact composition and performance parameters vary by manufacturer and edition; verify against your method.

Synthetic Utility

Not applicable as a chemical building block. Lactose Broth (USP) is a microbiological medium and is not used as a reagent or substrate in organic synthesis.

For chemical synthesis involving carbohydrates or peptides, select defined reagents (e.g., lactose monohydrate, protected sugars, amino acid derivatives) rather than complex nutrient blends.

Target Specificity

Not an antibody or affinity reagent. Target specificity does not apply.

Contextual note (literature): As a non‑selective enrichment medium, Lactose Broth supports a broad range of bacteria, with observational “specificity” arising only from the organism’s ability to ferment lactose (e.g., coliforms). Confirmatory testing on selective/differential media is required for identification.