LY2922470 - 10mM in DMSO , CAS No.1423018-12-5

CAS: 1423018-12-5 Cat. No.: L654938 Fórmula: C₂₈H₂₉NO₄S Peso molecular: 475.60 PubChem CID: 71257551

GRADE & PURITY 10mM in DMSO

Storage

Protected from light,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

LY2922470 is a potent, selective and orally available agonist of the G protein-coupled receptor 40 (GPR40) , with EC 50 s of 7 nM, 1 nM and 3 nM for human GPR40, mouse GPR40 and rat GPR40, respectively. LY2922470 reduces glucose levels along with significant increases in insulin and GLP-1 , is potential for the treatment of type 2 diabetes mellitus (T2DM)

IC50& Target:EC50：7 nM (human GPR40), 1 nM (mouse GPR40), 3 nM (rat GPR40)

Specifications

Especificaciones y pureza

10mM in DMSO

Mecanismos bioquímicos y fisiológicos

LY2922470 is a potent, selective and orally available agonist of the G protein-coupled receptor 40 (GPR40) , with EC 50 s of 7 nM, 1 nM and 3 nM for human GPR40, mouse GPR40 and rat GPR40, respectively. LY2922470 reduces glucose levels along with signific

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -80°C

Enviado en

Dry ice packs + Cold packs

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Tipo de acción

AGONIST

Nombres e identificadores

Isómeros SMILES

CC#C[C@@H](CC(=O)O)C1=CC=C(C=C1)OCC2=CC=C(S2)CN3CCCC4=C3C(=CC=C4)OC

CAS alternativo

1423018-12-5

PubChem CID

71257551

Términos de entrada MeSH

LY2922470

Peso molecular

475.60

Objetivos asociados (humanos)

FFAR1 Tchem Free fatty acid receptor 1 (3 Activities)

Discover Target: FFAR1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

No validated, item-specific experimental protocols are provided for LY2922470 in this listing.

General small-molecule screening workflow (for adaptation as needed):

• Stock preparation: Dissolve in an appropriate solvent (commonly dry DMSO) to a verified concentration (e.g., 10 mM). Filter if needed (0.22 µm PTFE) after confirming low adsorption.
• Aliquoting: Dispense single-use aliquots in amber vials or plates to avoid repeated freeze–thaw and light exposure.
• Assay setup: Perform serial dilutions maintaining constant vehicle across wells; include positive/negative controls and blanks. Equilibrate solutions to room temperature before dispensing to minimize precipitation caused by temperature gradients.
• Data quality: Monitor Z′-factor/assay window; check for precipitation or color/fluorescence interference.

Always tailor parameters (solvent type, dilution series, incubation time/temperature, detection wavelength) to your assay and to stability/solubility confirmed from the CoA/SDS.

Biological Roles

Item-specific biological target, pathway, or mechanism of action for LY2922470 is not provided in this listing.

What researchers can do (general guidance):

• Consult public databases using CAS 1423018-12-5 or PubChem CID 71257551 to identify reported targets or pathways. Verify consistency with your lot documentation before relying on literature claims.
• Profile activity using orthogonal assays (biochemical vs. cellular) to distinguish direct target engagement from pathway-level or phenotypic effects.
• Consider target deconvolution strategies if used as a phenotypic probe: chemoproteomics (affinity capture/thermal proteome profiling), CRISPR knockouts/knockdowns, or transcriptomic signatures.
• Assess ADME-relevant liabilities that may confound readouts (e.g., solubility limits, aggregation, redox activity, fluorescence interference) using counter-screens.

Notes and caveats:

• Do not infer clinical or therapeutic relevance from research-grade material. Any biological roles must be confirmed experimentally under your specific conditions.
• Maintain constant vehicle concentration and include appropriate controls to account for solvent or assay component effects.

Summary:

• Biological function and specificity for this item are not specified here; use literature resources for hypothesis generation and validate experimentally with rigorous controls.

Buffer Applications

This product is a small-molecule screening compound, not a buffering agent. Therefore, conventional buffer preparation guidance (pH range, capacity, recipes) does not apply.

Practical notes for assay buffers (general):

• Choose buffer systems that maintain stability and solubility of LY2922470 under your assay conditions once its ionization profile is confirmed from the CoA or literature.
• Keep co-solvent (e.g., DMSO) percentage constant across all samples and controls. Include carrier proteins (e.g., 0.1% BSA) or mild nonionic surfactants when appropriate to reduce nonspecific adsorption.
• Verify compatibility of buffer components (salts, detergents, reducing agents) with your detection modality to avoid artifacts.

Green Alternatives

While LY2922470 is a defined small-molecule research compound (not a solvent or reagent), greener choices can be made around its handling and workflow.

Greener dissolution/assay media (general guidance):

• Prefer aqueous buffers with minimal co-solvent when solubility permits. If co-solvent is required, evaluate ethanol or propylene carbonate as alternatives to high DMSO fractions, balancing assay tolerance and solubility.
• Consider cyclodextrin complexation (e.g., HP-β-CD) to reduce organic co-solvent usage for hydrophobic analytes.

Process choices and waste minimization:

• Scale solutions to immediate need to reduce expired stock disposal.
• Use 96/384-well plate formats that minimize dead volume; adopt automated low-volume dispensing to cut solvent waste.
• Consolidate compatible organic wastes; segregate halogenated streams to enable optimized treatment.

Comparison snapshot (general, not item-specific):

• DMSO: Excellent solvency and low volatility; challenging biodegradability; generally low toxicity but can enhance dermal uptake—use minimal effective %.
• Ethanol: Renewable feedstock, readily biodegradable; higher volatility/flammability; may limit solubility.
• Propylene carbonate: Low vapor pressure, favorable environmental profile; viscosity higher than DMSO; assay compatibility to be verified.

Packaging/handling:

• Reuse amber glass vials where validated; employ cold-chain shippers designed for recyclability. Limit dry-ice use by planning consolidated shipments while maintaining product integrity.

Pharmaceutical Uses

This listing supplies LY2922470 strictly for research use. No pharmacopoeial status, excipient role, or formulation use is specified for this item.

General considerations (for preformulation research only):

• If used in formulation feasibility studies, determine the confirmed salt form, polymorph, and purity from the CoA before conducting solubility/stability screens.
• For solution studies, characterize pH–solubility profile, cosolvent tolerance, and potential for cyclodextrin inclusion or lipid-based systems as needed. Document photostability given the light-protection requirement.
• Do not use this material for human or veterinary applications. It is not intended for clinical, diagnostic, or household use.

Physical Properties

Item-specific physicochemical data are not provided in this listing.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point / Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD, pKa: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice, not item-specific):

• Small-molecule screening compounds are commonly supplied as solids intended to be reconstituted in an aprotic polar solvent (e.g., DMSO) to make concentrated stocks (e.g., 10–50 mM) for HTS or biochemical assays. Verify actual solubility for LY2922470 from the CoA prior to use.
• If UV–Vis quantification is intended, determine the compound’s wavelength of maximum absorbance and extinction coefficient experimentally; do not assume generic values.
• Hygroscopicity, polymorphism, or light-sensitivity may affect handling; for this item, protection from light and deep-freeze storage are specified (see Storage & Reconstitution). Conduct small-scale pretests to confirm dissolution kinetics and stability under your assay conditions.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and best practices:

• Screening/Library compounds are typically supplied at high chemical purity suitable for discovery assays (commonly ≥95% by HPLC), but do not assume this for LY2922470—confirm on the lot-specific CoA, including assay method (e.g., HPLC-UV, ELSD, or qNMR) and reporting basis (area% vs. weight%).
• Identity confirmation: Review provided spectra/chromatograms. Ideally confirm by LC–MS and, if critical, 1H/13C NMR against reference data.
• Residual solvents, water content, and counter-ions/salt form can influence assay results. If these may impact your application, request the detailed specification or analyze in-house (e.g., KF for water, GC for residuals).
• Stabilizers: Not specified for this item; refer to CoA/Spec Sheet. If present, document compatibility with your assay readout.
• Light and freeze stability: Product Data specifies protection from light and −80 °C storage. Consider accelerated stability checks (e.g., 24–48 h at 4–25 °C) to understand bench-time limits for your workflow.
• Batch-to-batch consistency: For SAR or HTS, lock to a single lot when possible; otherwise, bracket with QC checks (retention time, purity, potency) to ensure continuity.

Reaction and Applications

This product is provided for use as a research small molecule (screening/assay) rather than as a synthetic reagent.

Typical research applications (general, non–item-specific):

• High-throughput screening (HTS), fragment or focused library testing, and hit confirmation using biochemical, biophysical (e.g., thermal shift, SPR), or cell-based assays.
• Chemical biology: Probe development, pathway interrogation, and phenotypic screening with subsequent target deconvolution.
• Method development: Assay optimization for DMSO tolerance, adsorption mitigation, and plate handling.

Assay design tips (practice-based):

• Prepare concentrated DMSO stock (e.g., 10 mM), then perform serial dilutions to achieve a consistent vehicle concentration across all wells (commonly 0.1–0.5% v/v DMSO).
• Verify solubility at working concentrations and under assay buffer conditions; check for precipitation after dilution and during incubation.
• Mitigate nonspecific binding: Use low-binding polypropylene plates/tubes; include a small amount of nonionic surfactant (e.g., 0.01–0.05% Tween-20) when compatible.
• Light sensitivity: Since protection from light is specified, limit light exposure during preparation and assays (use amber vials/plates).
• Stability: Establish bench stability windows (e.g., repeated reads at 0, 2, 4, 24 h) and freeze–thaw tolerance using LC–MS.

Controls:

• Include vehicle-only controls, positive/negative assay controls, and, when possible, orthogonal readouts to rule out assay interference (e.g., redox cycling, fluorescence quench/enhance).

Reaction Conditions

Not typically applicable. LY2922470 is supplied as a research compound for screening and assay use, not as a reagent or catalyst with defined reaction condition envelopes.

General laboratory handling conditions (non-synthetic):

• Solution preparation at ambient temperature in low-light conditions is recommended due to the stated light sensitivity.
• Avoid elevated temperatures unless stability has been verified by analytical methods (e.g., LC–MS after 1–2 h at 37 °C in the intended matrix).

Safety and Handling

Safety information provided on this page is limited; always defer to the SDS for authoritative, lot-specific guidance.

• Research use: For research use only.
• GHS classification: Not specified for this item; refer to SDS.
• Signal word / Hazard (H) statements / Pictograms: Not specified for this item; refer to SDS.
• Known storage precautions (from Product Data):
  • Store at −80 °C.
  • Protect from light.
  • Shipped on dry ice packs + cold packs to maintain cold chain.

General laboratory precautions (good practice):

• PPE: lab coat, safety glasses, and suitable chemically resistant gloves. Handle powders/solutions in a fume hood to avoid inhalation/dermal exposure.
• Incompatibilities: Unknown for this specific item; until confirmed by SDS, avoid strong oxidizers and reactive bases/acids. Keep away from light as indicated.
• Spill/first aid (overview; consult SDS):
  • Skin/eye contact: Rinse with water for ≥15 minutes; remove contaminated clothing; seek medical attention as needed.
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Ingestion: Rinse mouth; get medical advice.
• Special risks: For small molecules in DMSO, note rapid dermal absorption potential—avoid skin contact with solutions. Prevent repeated freeze–thaw to minimize aerosolization and container stress.

Waste: Dispose of in accordance with institutional and local regulations; segregate halogenated/non-halogenated solvent waste as applicable.

Solvent Selection

Item-specific solubility data are not provided. The guidance below reflects common practice for small-molecule screening compounds.

• Primary solvent for stock solutions (general):
  • DMSO: Widely used for 10–50 mM stocks due to broad solvency and assay compatibility at ≤0.5–1% v/v final concentration.
• Secondary solvents/co-solvents (as needed):
  • DMF or NMP (for challenging solutes; verify assay tolerance), ethanol, methanol, or acetonitrile.
  • Aqueous buffers with co-solvent and/or pH adjustment if ionizable groups are confirmed from the CoA.
• Solubility verification: Conduct a small-scale shake-flask or microplate solubility test at target stock concentration; inspect for haze/precipitate after 15–30 min and after freeze–thaw.

Practical tips:

• Warm to ambient and vortex/sonicate to assist dissolution; avoid prolonged heating or light exposure.
• Filter 0.22 µm if particulate persists and the compound is not adsorptive; pre-rinse filters with solvent to minimize analyte loss.
• Minimize water uptake in DMSO by working quickly in low humidity; cap immediately.

Comparison (general considerations):

• DMSO vs ethanol: DMSO typically provides higher solubility but can affect some cell-based assays at lower percentages; ethanol is more volatile and may introduce evaporation/edge effects.
• Acetonitrile vs methanol: Both are miscible with water and support LC workflows; methanol may better solubilize some polar/heteroatom-rich scaffolds.

Storage and Reconstitution

Authoritative item-specific instructions (from Product Data):

• Storage: Store at −80 °C.
• Light sensitivity: Protect from light (use amber vials/foil wrap; minimize exposure during weighing and solution prep).
• Shipping: Shipped with dry ice packs + cold packs to maintain the cold chain.

General reconstitution guidance (apply after confirming solubility on the CoA):

• Solvent: Use anhydrous DMSO for initial stock solutions unless otherwise specified. Alternative solvents may be used if validated for your assay.
• Concentration: Common practice is 10 mM stocks for HTS. Adjust based on actual solubility and assay needs.
• Technique: Allow vial to equilibrate to room temperature in the dark before opening to avoid moisture condensation. Weigh quickly; cap promptly. Vortex/sonicate gently to aid dissolution.
• Aliquoting: Prepare single-use aliquots in amber, low-binding tubes; purge headspace with inert gas if oxidative or hydrolytic lability is suspected.
• Freeze–thaw: Avoid repeated cycles; store aliquots at −80 °C. Record bench-time limits based on internal stability checks.
• Short-term handling: Keep on ice or at 2–8 °C during assay setup if stability at room temperature is unverified.

Documentation: Retain CoA/SDS with the lot; annotate preparation date, solvent, concentration, and number of freeze–thaw events for data integrity.

Structure and Identity

Brief overview: LY2922470 is offered as a research-only small-molecule screening compound in our compound library portfolio.

• SKU: L654938
• Product name: LY2922470
• CAS: 1423018-12-5
• PubChem CID: 71257551 (for public structural reference)
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general notes):

• Item-specific 2D/3D structure is not provided in this listing. For researchers needing exact atom connectivity, stereochemistry, and functional group annotation, consult the Certificate of Analysis (CoA) or authoritative databases (e.g., PubChem CID 71257551) and verify against your internal acceptance criteria.
• Without a confirmed structure on the product page, avoid inferring functional groups or reactivity profiles; base any assay design on verified structural data from the CoA/SDS bundle.

Identity best practices:

• Cross-check CAS and lot-specific identifiers on receipt.
• Confirm identity by orthogonal methods when critical (e.g., LC–MS, HRMS, 1H/13C NMR, and HPLC/UPLC retention time vs. reference), especially prior to SAR or mechanistic studies.

Synthetic Utility

LY2922470 is provided as a finished small-molecule research compound rather than a synthetic building block or reagent. As such, it is not typically employed as a starting material or intermediate in routine synthesis.

If derivatization is scientifically justified (general guidance):

• Consult the definitive structure (CoA/authoritative database) to identify modifiable positions and functional groups.
• Plan orthogonal protection and mild transformation conditions to preserve sensitive motifs (especially if light-sensitive behavior suggests photolabile features).
• Validate structural integrity post-modification with LC–MS and NMR, and re-establish purity and activity profiles before use as a probe analog.

For most users, the primary value of this item is in biological/biochemical evaluation rather than in synthetic transformations.

Target Specificity

Target, selectivity profile, and potency data are not provided for this item.

• Antigen/epitope/clone/isotype: Not applicable (this is a small molecule, not an antibody or protein reagent).
• If target-specific use is intended, consult peer-reviewed literature via CAS 1423018-12-5 or PubChem CID 71257551 and verify findings with your lot via in-house assays.
• Incorporate orthogonal assays and counter-screens to confirm specificity and to identify potential off-target or assay-interference effects.