MAD2L1BP Mouse mAb - Anticuerpo primario, specific to MAD2L1BP

Cat. No.: Ab325658

GRADE & PURITY KD Validation

Application

Flow Cytometry, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Conjugation

Unconjugated

Specifications

Product Name

MAD2L1BP Mouse mAb - Anticuerpo primario, specific to MAD2L1BP

Especificaciones y pureza

KD Validation

Especies hospedadoras

Ratón

Especificidad

MAD2L1BP

Inmunógeno

Recombinant protein of human MAD2L1BP

Conjugación

Unconjugated

Grado

KD Validation

Método de purificación

Protein A/G purified

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C for one year.

Application Protocols

Tested/validated applications and recommended conditions are not provided in the product data for this item. Refer to the lot-specific CoA/Spec Sheet for:

• Tested applications (e.g., WB, IF, IHC, IP, ELISA) and positive control lysates/tissues
• Recommended starting dilutions and incubation conditions
• Sample preparation tips (lysis buffers, fixation methods)
• Secondary antibody recommendations and detection systems

General starting points (not item-specific; for pilot optimization only)

• WB: 1:1000 in TBS-T with 5% BSA; incubate overnight at 4 °C; use mitotic cell lysates as potential positives.
• IF: 1:200 in PBS with 1% BSA; fix with PFA and permeabilize with 0.1% Triton X-100; include no-primary control.
• IP: 1–5 µg antibody per mg total protein; capture with Protein A/G resin; validate specificity by KD/KO control when possible.

These are generic guidelines; prioritize the CoA/Spec Sheet for item-specific protocols.

Biological Roles

Target context (literature; not item-specific performance claims)

• MAD2L1BP, also known as p31comet, is a spindle assembly checkpoint (SAC) regulator that antagonizes MAD2 function to promote checkpoint silencing and anaphase onset. It binds the closed conformation of MAD2 and participates in disassembly of the mitotic checkpoint complex (MCC), facilitating APC/C activation and progression through mitosis.
• By modulating the SAC, MAD2L1BP contributes to fidelity of chromosome segregation. Perturbation of MAD2L1BP levels can alter mitotic timing, affect MCC stability, and influence genome stability in proliferating cells (literature).
• Interactions: MAD2L1BP interacts with MAD2L1 and other checkpoint components; its recruitment and activity are regulated temporally during mitosis (literature). Post-translational modifications (e.g., phosphorylation) have been reported to influence function and localization in some systems.

Experimental applications enabled by anti-MAD2L1BP antibodies (general guidance)

• Western blotting to assess MAD2L1BP expression across cell cycle stages or following RNAi/CRISPR perturbations.
• Immunoprecipitation to study complexes with SAC proteins under nocodazole- or taxol-induced arrest.
• Immunofluorescence to visualize mitotic localization patterns in fixed cells.

Note: The above describes the biology of the antigen. This product’s tested applications, species reactivity, and validated use cases are not specified here; consult the CoA/Spec Sheet for item-specific validation and performance data.

Buffer Applications

Antibody performance is highly dependent on buffer composition. No item-specific buffer is provided for this product; refer to the CoA/Spec Sheet. General, practice-tested guidance follows.

• Stock/storage buffer (general): Many IgG preparations are supplied in PBS (10–50 mM phosphate, 150 mM NaCl, pH 7.2–7.4) with stabilizers (e.g., 0.02–0.05% sodium azide, 10–50% glycerol) to support freeze–thaw stability. This item’s exact buffer is not specified.
• Working buffers by assay (general):
  • Western blot: TBS-T (20–50 mM Tris, 150 mM NaCl, 0.05–0.1% Tween-20, pH 7.4–7.6); block with 3–5% BSA or milk (optimize to reduce background).
  • Immunofluorescence/IHC: PBS or TBS with 0.05% Tween-20 for washes; blocking with 1–5% BSA/serum. If azide is present, avoid HRP-based amplification without adequate washing.
  • ELISA: Coating in carbonate–bicarbonate buffer (pH ~9.6) for capture Ab; sample and detection in PBS/TBS with 0.05% Tween-20 and 1% BSA.
• Conjugation: Exchange into amine-free buffers (e.g., 50 mM PBS replaced with 50 mM sodium bicarbonate, pH 8.3) before NHS-ester labeling; remove azide prior to HRP conjugation.
• Storage additives (general): 10–50% glycerol permits −20 °C storage without full freezing; sugars (trehalose/sucrose) can improve freeze–thaw resilience.

Always confirm compatibility of buffers with the specific lot (preservatives, stabilizers, carrier protein) on the CoA/Spec Sheet.

Green Alternatives

Conventional green chemistry comparisons (e.g., replacing hazardous organic solvents) are not directly applicable to an aqueous monoclonal antibody reagent.

Sustainability-oriented best practices for antibody use (general)

• Use minimal effective volumes to reduce consumables and waste.
• Choose reusable containers/racks when compatible with sterility needs; minimize single-use plastics.
• Prefer non-mercury, non-chromate reagents in associated assays. If sodium azide is present as a preservative, collect azide-containing waste separately and avoid acidification.
• Cold-chain efficiency: Consolidate shipments and store promptly at the recommended temperature to reduce spoilage and repeat orders.

These are general recommendations. No item-specific green alternative requirements are specified; refer to institutional EHS guidance.

Pharmaceutical Uses

Not applicable. This product is designated “For research use only” and is not formulated, tested, or released for diagnostic or therapeutic applications.

General information (context; not item-specific claims)

• Monoclonal antibodies used in pharmaceutical manufacturing require GMP production, defined viral safety, extensive characterization (purity, charge/size variants, glycosylation), and compliance with pharmacopeial/ICH guidelines—none of which are implied by this research-grade reagent.
• Excipient systems for clinical formulations (e.g., histidine, sucrose/trehalose, polysorbate 80) differ from common research buffers and are selected via developability studies. Such formulation considerations do not apply to this catalog item.

For any translational intent, procure appropriate GMP-grade materials and follow regulatory guidance.

Physical Properties

Antibodies are aqueous protein preparations; exact physicochemical parameters depend on formulation and concentration.

Item-specific values

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration: Not specified for this item; refer to CoA/Spec Sheet.
• Formulation/buffer, stabilizers, and preservative: Not specified for this item; refer to CoA/Spec Sheet.
• pH and osmolality: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance (for context; not item-specific specifications)

• Typical IgG molecular mass: ~150 kDa (literature).
• Solubility: IgGs are water-soluble; stability depends on pH (~6.5–7.5 common), ionic strength, and excipients (literature).
• Isoelectric point (pI): IgGs often pI ~6–9 depending on subclass/sequence (literature).
• Thermal behavior: Denaturation/onset typically >60 °C but varies with buffer and concentration (literature); avoid heat exposure.
• Light sensitivity: Aromatic residues and disulfides can undergo photo-oxidation; store protected from light when possible (literature).

Practical notes

• Avoid repeated freeze–thaw cycles to minimize aggregation and activity loss (item instruction aligns with best practice).
• Gentle handling (no vigorous vortexing) and use of low-protein-binding plastics/glass helps maintain integrity.

Where precise values are required for assay design (e.g., extinction coefficient for A280 quantitation), consult the lot-specific CoA/Spec Sheet.

Quality and Grades

Item-specific quality note

• Grade/Purity: KD Validation (per product data). While not a standardized pharmacopeial grade, “KD Validation” commonly indicates the antibody has been functionally evaluated in a gene knockdown/knockout (KD/KO) context to confirm target specificity in a defined application. The exact scope (cell line, method, assay type) is not specified for this item; refer to CoA/Spec Sheet for validation details and representative data.

What KD/KO validation implies (general guidance)

• Specificity assessment: Signal reduction/ablation upon silencing MAD2L1BP supports on-target binding and helps rule out major off-target recognition (literature practice).
• Reproducibility: Inclusion of genetic controls improves lot-to-lot and assay-to-assay confidence compared to reliance on peptide competition alone.
• Limitations: Validation is application- and condition-specific. Performance in WB may not translate to IHC/IF or IP without re-optimization.

Additional quality parameters to review per lot (not specified here)

• Clone ID, isotype/subclass, source (hybridoma vs recombinant), concentration, purity (e.g., Protein A/G), endotoxin level, aggregation profile, preservative content, and storage buffer. For chromatography-based or MS workflows, low BSA/carrier-free formats may be preferred—confirm on the CoA.

Recommendation

• Before critical experiments, verify the lot-specific validation data, application suitability, and any tested species/applications listed on the CoA/Spec Sheet.

Reaction and Applications

This section is not typically applicable to monoclonal antibodies, which are biological affinity reagents rather than small-molecule reactants or catalysts.

Guidance

• For intended laboratory uses (e.g., Western blot, immunofluorescence, immunoprecipitation, ELISA), consult the Application Protocols tab and the lot-specific CoA/Spec Sheet for tested applications and recommended dilutions.
• If the antibody is to be conjugated (e.g., HRP/biotin/fluorophore), select carrier-free formats where possible and use appropriate chemistries (e.g., NHS ester labeling) with buffer exchange into amine-free buffers.

No item-specific application data were provided in the product data; see CoA/Spec Sheet.

Reaction Conditions

Not applicable to chemical reactions. Antibody usage conditions are assay-specific rather than governed by classical reaction parameters.

General assay-condition guidance (not item-specific)

• Temperature: Perform antibody incubations at room temperature or 4 °C depending on assay and background; extended incubations at 4 °C often improve specificity.
• Time: Primary antibody incubations typically range from 1–2 h (RT) to overnight (4 °C); secondary incubations are commonly 45–90 min (RT).
• Concentration: Optimize empirically; starting dilutions for WB often 1:500–1:2000; for IF/IHC 1:100–1:500; for IP 1–5 µg per mg lysate. Exact recommendations for this item are not specified; consult CoA/Spec Sheet.

For validated, item-specific conditions, refer to the lot-specific documentation.

Safety and Handling

Safety data

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS for authoritative information.
• Research use: For research use only (per product data). Not for diagnostic or therapeutic use.

General laboratory precautions for antibodies (guidance)

• PPE: Wear lab coat, gloves, and safety glasses. Avoid inhalation of aerosols and contact with skin/eyes.
• Handling: Work on ice for prolonged bench manipulations. Do not vortex vigorously. Avoid repeated freeze–thaw cycles.
• Incompatibilities: Avoid strong oxidizers and extreme pH. Preservatives (if present, e.g., sodium azide) are toxic; prevent contact with acids to avoid hydrazoic acid formation in plumbing.
• Biological safety: Although recombinant/hybridoma-derived antibodies are typically low risk, treat as potentially biohazardous protein solutions. Decontaminate spills with suitable disinfectant (e.g., 0.1% sodium hypochlorite), then rinse.
• First aid (general): In case of skin/eye contact, rinse with water for 15 minutes and seek medical advice if irritation persists. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical attention. Provide SDS to responders.
• Waste: Dispose of proteinaceous waste and azide-containing solutions according to institutional and local regulations.

Always consult the product-specific SDS for hazards, first aid measures, exposure controls, and disposal guidance.

Solvent Selection

Not typically applicable. As a protein reagent, this mouse monoclonal antibody is supplied in an aqueous buffer rather than used with organic solvent systems.

Practical guidance (general, not item-specific)

• Preferred diluents: PBS or TBS at physiological ionic strength, with optional 0.05–0.1% Tween-20 for blot washes. Include a suitable protein carrier (e.g., 1–5% BSA) for blocking and to minimize nonspecific adsorption, unless a carrier-free format is required for conjugation.
• Avoid: Strong organic solvents, extremes of pH, and high chaotrope concentrations unless specifically required for elution (e.g., IP). If antibody is azide-containing, avoid buffers destined for live-cell applications.
• Filtration: 0.22 µm filtration can reduce bioburden; avoid shear/pressure that could denature IgG.

For formulation/dilution details of this specific item (buffer composition, preservative, recommended diluent), refer to the CoA/Spec Sheet.

Storage and Reconstitution

Storage (item-specific)

• Store at −20 °C (per product data).
• Avoid repeated freezing and thawing (per product data). Aliquot upon first thaw to minimize freeze–thaw cycles.
• Shipment: Ice chest + ice pads (per product data). Inspect upon receipt; return to recommended storage promptly.

General best practices (not item-specific)

• If supplied in a glycerol-containing buffer (not specified for this item), storage at −20 °C without full freezing is common. If supplied without glycerol, consider aliquoting and storing at −80 °C for long-term stability.
• Protect from microbial contamination. Use sterile, low-protein-binding tubes. Avoid prolonged exposure to room temperature.
• Do not heat-inactivate; elevated temperatures can irreversibly denature IgG.

Reconstitution

• If provided lyophilized (not specified for this item), reconstitute with sterile water or the recommended buffer to the stated concentration; gently invert to mix. Allow complete dissolution on ice, then aliquot.
• For buffer exchange or concentration, use centrifugal ultrafiltration with an appropriate MWCO (e.g., 30–50 kDa for IgG), pre-rinsed to remove glycerol/detergents if incompatible with downstream assays.

For the exact formulation, concentration, and any stabilizers/preservatives present in this product, consult the CoA/Spec Sheet.

Structure and Identity

This product is a mouse monoclonal antibody (mAb) directed against the protein MAD2L1BP.

• Item type: Monoclonal antibody, mouse origin (Ig class/isotype not specified for this item; refer to CoA/Spec Sheet.)
• Antigen target: MAD2L1BP (target name per product title)
• Molecular formula: Not applicable to protein mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. Typical mouse IgG is ~150 kDa (literature).
• SMILES / InChI / InChIKey: Not applicable for antibodies.
• Structural features (general, literature): Immunoglobulins are Y‑shaped glycoproteins composed of two heavy and two light chains with variable domains (VH/VL) forming the antigen-binding site (paratope) that recognizes a specific epitope on the target antigen.
• 2D description (general): The antibody comprises a Fab region (antigen binding) and an Fc region (effector), connected via hinge. As a monoclonal reagent, all molecules share identical binding site sequences (clone not specified for this item).

Notes specific to this item

• Clone ID, isotype, light chain type, and epitope mapping: Not specified for this item; refer to CoA/Spec Sheet.
• Supplied buffer, concentration, and format: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. As a biological affinity reagent, this monoclonal antibody is not used as a synthetic intermediate, reagent, or catalyst in organic synthesis.

For experimental use, see Biological Roles, Buffer Applications, and Application Protocols (when available on the CoA/Spec Sheet).

Target Specificity

Only information from product data is provided here.

• Target antigen: MAD2L1BP
• Antibody type: Mouse monoclonal antibody (per product title)
• Clone ID: Not specified for this item; refer to CoA/Spec Sheet.
• Isotype/subclass: Not specified for this item; refer to CoA/Spec Sheet.
• Epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity (tested): Not specified for this item; refer to CoA/Spec Sheet.
• Cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• KD/KO validation: Grade indicates KD Validation; details not specified for this item; refer to CoA/Spec Sheet.

Preguntas frecuentes

What does KD Validation mean?

KD Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.