CALIDAD & PUREZABioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Ready-to-use?Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors.Suitable for Immunohistochemistry(IHC)?IHC grade — validated for immunohistochemistry on tissue sections. Use to detect and localize antigens in fixed tissue.
BioReagent, ready-to-use, Suitable for Immunohistochemistry BioReagent,Listo para usar,Suitable for Immunohistochemistry(IHC) para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Protected from light,Room temperature Envío Normal. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Methylgreen-pyronine is a common dye used in tissue or cell staining to color the nucleus green or blue-green and the nucleoli in the cytoplasm and nucleus red. Methyl green can bind to DNA in the nucleus, thus producing nuclear staining; Pyronin can bind to RNA in the cytoplasm or nucleolus, so that the cytoplasm and nucleolus can be stained. Operation process: 1 Sample handling 1.1 For paraffin sections: dewaxing in xylene for 2×5min; Anhydrous ethanol 5 min, 90% ethanol 2 min, 70% ethanol 2 min; Rinse with PBS for 2 min. 1.2 For frozen sections: Rinse with PBS for 2 min. 1.3 For cultured cells: fixed with 4% paraformaldehyde for more than 10 min and washed with PBS for 2×2 min. 2 methyl green-Pyronine staining for the above treated samples: 2.1 Methyl green-Pyronine dyeing solution staining for about 5 ~10min (the time can be adjusted according to the dyeing results and requirements). 2.2 Wash distilled water 2-3 times (each time for seconds). 3 Dehydration, transparency, sealing or other dyeing 3.1 Dehydration of acetone 3 times, 1~2sec(3 dips) each time. 3.2 Wash with acetone/xylene (1:1) twice, 1~2min each time. Xylene transparent 2×1~2min. 3.3 Seal the tablet with neutral gum or other sealant. 3.4 Microscopically, the nucleus appears green or blue-green, while the cytoplasm and nucleolus appear red. Staining results: the nucleus is dyed green or blue-green, and the nucleoli in the cytoplasm and nucleus are dyed red. Note: 1 Need to bring 4% polyformaldehyde, gradient ethanol, xylene, neutral gum or other tablets. 2 When using this kit for the first time, it is recommended to take 1 to 2 samples for pre-test. Warm reminder: For your own safety, please take good protection before using the reagent, such as wearing a lab coat, gloves, etc.
Especificaciones
Sinónimos
Methyl Green-Pyronin Staining Solution
Especificaciones y pureza
BioReagent, ready-to-use, Suitable for Immunohistochemistry
Estabilidad y almacenamiento
Store at Room temperature long term (12 months). Upon receipt, it is recommended to aliquot. Store in the dark.
Condiciones de almacenamiento de almacenamiento
Protected from light,Room temperature
Enviado en
Normal
Grado
BioReagent, Listo para usar, Suitable for Immunohistochemistry(IHC)
Product Line
Productos biológicos
Documentación
📋 Hoja de datos de seguridad (SDS)
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
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