≥96% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 6 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Methylene blue is used as a dye in different staining procedures viz. Wright's stain and Jenner's stain. It also serves as an indicator and medicine. Further, it is used to examine RNA and DNA under the microscope or in a gel. It is widely used as a redox indicator in analytical chemistry. It acts as a photosensitizer utilized to generate singlet oxygen in order to make organic peroxides by a Diels-Alder reaction. It is also used in the sulfide analysis. It plays an important role in aquaculture to treat fungal infections.
| Pubchem Sid | 488199112 |
|---|---|
| Pubchem Sid Url | https://pubchem.ncbi.nlm.nih.gov/substance/488199112 |
| Canonical Smiles | CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.[Cl-] |
| IUPAC Name | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride;hydrate |
| InChIKey | WQVSELLRAGBDLX-UHFFFAOYSA-M |
| INCHI | 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1 |
| Isomeric SMILES | CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.[Cl-] |
| Molecular Weight | 319.85 (anhydrous basis) |
| Reaxy-Rn | 29647196 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=29647196&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Taxonomy Tree
| Kingdom | Organic compounds |
|---|---|
| Superclass | Organoheterocyclic compounds |
| Class | Benzothiazines |
| Subclass | Not available |
| Intermediate Tree Nodes | Not available |
| Direct Parent | Benzothiazines |
| Alternative Parents | Dialkylarylamines Benzenoids Heteroaromatic compounds Azacyclic compounds Organopnictogen compounds Organic oxides Organic chloride salts Hydrocarbon derivatives |
| Molecular Framework | Aromatic heteropolycyclic compounds |
| Substituents | Benzothiazine - Dialkylarylamine - Tertiary aliphatic/aromatic amine - Benzenoid - Heteroaromatic compound - Tertiary amine - Azacycle - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organic chloride salt - Organic salt - Organonitrogen compound - Amine - Aromatic heteropolycyclic compound |
| Description | This compound belongs to the class of organic compounds known as benzothiazines. These are organic compounds containing a benzene fused to a thiazine ring (a six-membered ring with four carbon atoms, one nitrogen atom and one sulfur atom). |
| External Descriptors | Not available |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 K2021072 | Certificate of Analysis | Jun 11, 2026 | M190219 |
| B2314127 | Certificate of Analysis | Oct 30, 2025 | M190219 |
| B2314116 | Certificate of Analysis | Oct 30, 2025 | M190219 |
| B2314112 | Certificate of Analysis | Oct 30, 2025 | M190219 |
| I2519017 | Certificate of Analysis | Sep 30, 2025 | M190219 |
| J2213165 | Certificate of Analysis | Jul 10, 2025 | M190219 |
| F2223402 | Certificate of Analysis | Apr 03, 2025 | M190219 |
| F2223401 | Certificate of Analysis | Apr 03, 2025 | M190219 |
| F2223387 | Certificate of Analysis | Apr 03, 2025 | M190219 |
| K2112508 | Certificate of Analysis | Aug 21, 2024 | M190219 |
| F2223384 | Certificate of Analysis | Jun 01, 2022 | M190219 |
| E2410046 | Certificate of Analysis | Jun 01, 2022 | M190219 |
| F2121325 | Certificate of Analysis | May 06, 2021 | M190219 |
| I2311055 | Certificate of Analysis | May 06, 2021 | M190219 |
| F2121324 | Certificate of Analysis | May 06, 2021 | M190219 |
| F2121323 | Certificate of Analysis | May 06, 2021 | M190219 |
| F2121322 | Certificate of Analysis | May 06, 2021 | M190219 |
| Solubility | Soluble in water, alcohol, acetic acid, glycerol and chloroform. Insoluble in ether. |
|---|---|
| Melt Point(°C) | 180℃ |
| Molecular Weight | 337.900 g/mol |
| XLogP3 | |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 1 |
| Exact Mass | 337.102 Da |
| Monoisotopic Mass | 337.102 Da |
| Topological Polar Surface Area | 44.900 Ų |
| Heavy Atom Count | 22 |
| Formal Charge | 0 |
| Complexity | 483.000 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| The total count of all stereochemical bonds | 0 |
| Covalently-Bonded Unit Count | 3 |
| 1. Amir Said, Chang Gao, Caiyun Liu, Huihui Niu, Dexin Wang, Yanshu Liu, Lin Du, Chen-Ho Tung, Yifeng Wang. (2021) A Mesoporous Lead-Doped Titanium Oxide Compound with High Performance and Recyclability in I2 Uptake and Photocatalysis. INORGANIC CHEMISTRY, [PMID:34955011] [10.1021/acs.inorgchem.1c03263] |
| 2. Mingqiang Liu, Baochang Zhang, Huicai Wang, Fei Zhao, Yuan Chen, Qiang Sun. (2016) Facile crosslinking synthesis of hyperbranch-substrate nanonetwork magnetite nanocomposite for the fast and highly efficient removal of lead ions and anionic dyes from aqueous solutions. RSC Advances, 6 (71): (67057-67071). [PMID:] [10.1039/C6RA11037A] |
| 3. Wang Pan, Li Huijuan, Zhang Xingjiang, Wang Xiaoxun, Sun Wenwen, Zhang Xiaoya, Chi Baiyi, Go Yuyo, Chan Xi Hui Felicia, Wu Jianxin, Huang Qing. (2025) Microecology in vitro model replicates the human skin microbiome interactions. Nature Communications, 16 (1): (1-14). [PMID:40164644] [10.1038/s41467-025-58377-2] |
| 4. Xiaole Yan, Meiling Chen, Junning Wang, Zhaodong Wang, Ruiqi Xin, Dujuan Wu, Ye Song, Song Li, Wenkai Zhu, Chaohai Wang, Yanli Mao. (2024) Nanoarchitectonics of bamboo-based heterojunction photocatalyst for effective removal of organic pollutants. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2024.153431] |
| 5. Manman Xie, Canran Jiang, Cong Zhang, Yun Wu, Xiuli Zhang, Ruosi Yao, Cuiping Han, Yue Dai, Kai Xu, Shaohui Zheng. (2025) Tumor microenvironment triggered iron-based metal organic frameworks for magnetic resonance imaging and photodynamic-enhanced ferroptosis therapy. JOURNAL OF COLLOID AND INTERFACE SCIENCE, [PMID:39855085] [10.1016/j.jcis.2025.01.119] |
| 6. Le Yin, Mu Hu, Yajie Liu, Yiyi Lin, Yuan Wang, Shaojun Yuan. (2025) Smart Thermoresponsive CoZn-ZIF Decorated PVDF/PNIPAM Nanofiber Membrane for Switchable Emulsion Separation and Catalytic Dye Degradation. ACS Applied Materials & Interfaces, [PMID:41384348] [10.1021/acsami.5c16975] |
