Mitochondrial complex Ⅲ Activity Assay Kit (Micro Method)

Cat. No.: M1515824

GRADE & PURITY BioReagent

Descripción general

Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as coenzyme Q-cytochrome c reductase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is a common component of both the main and branch pathways of the mitochondrial respiratory electron transport chain, responsible for transferring hydrogen from reduced coenzyme Q to cytochrome c, thereby generating reduced cytochrome c. Mitochondrial respiratory chain complex III is a multisubunit membrane protein complex and serves as a fundamental component involved in both photosynthesis and the respiratory electron transport chain. The detection principle of this kit is based on the activity of mitochondrial respiratory chain complex III, which transfers hydrogen from reduced coenzyme Q to cytochrome c, producing reduced cytochrome c. Unlike oxidized cytochrome c, reduced cytochrome c exhibits characteristic light absorption at 550 nm. Therefore, the rate of increase in light absorption at 550 nm reflects the activity of mitochondrial respiratory chain complex III. By measuring the change in absorbance at 550 nm, the activity of mitochondrial respiratory chain complex III in the sample can be calculated.

Specifications

Especificaciones y pureza

BioReagent

Grado

BioReagent

Aplicación

Enzyme activity assay

Tipo de muestra

Tejidos animales, Tejidos vegetales

Método de detección

Colorimetric Method

Detection instrument

Microplate reader

Detection wavelength

550nm

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ for 6 months. Store in the dark.

Preguntas frecuentes

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.