Modified BPLS Agar - BioReagent

Cat. No.: M1528385

CALIDAD & PUREZA BioReagent

Storage

Room temperature

Shipped In

Normal

Descripción general

For selective isolation of Salmonella in samples

Especificaciones

Especificaciones y pureza

BioReagent

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Grado

BioReagent

Product Line

Productos biológicos

Application Protocols

Below is a general workflow for preparing and using agar-based microbiological media. Follow the lot-specific instructions on the label/CoA for this Modified BPLS Agar; do not substitute the values below for official directions.

A. Plate preparation (literature/general):

• Weigh the indicated grams of dehydrated medium per liter of purified water (value Not specified for this item; refer to CoA/Spec Sheet).
• Disperse in cold water, then heat with stirring to dissolve completely. Avoid boiling over.
• Sterilize as directed on the label (e.g., autoclave liquid cycle if specified). Some media are heat-labile and require alternative sterilization; defer to CoA.
• Cool to ~45–50 C, mix gently to homogenize, and pour aseptically into sterile Petri dishes. Allow to solidify and dry surface moisture before use.

B. Inoculation and incubation (literature/general):

• Streak, spread, or spot-inoculate plates as per method. Include positive and negative controls for growth-promotion/selectivity verification.
• Incubate under validated conditions (temperature, time, atmosphere) for the organisms of interest.

C. Reading results (literature/general):

• Examine colony morphology, color/halo development, and medium reaction according to your SOP. Record and, if required, confirm with biochemical or molecular tests.

Biological Roles

This is a formulated growth medium designed to support and differentially reveal microbial growth characteristics in research settings.

• Functional role: Provides macronutrients, minerals, and a solid matrix (agar) to support colony formation. Selective components (e.g., bile salts) and carbohydrate indicators (e.g., lactose/sucrose systems) are commonly employed in BPLS-type media to differentiate fermentation phenotypes (literature/general). The exact composition and selectivity of this Modified BPLS Agar are vendor-specific and not specified here.
• Mechanistic basis (literature/general):
  • Nutrient base supplies amino acids/peptides and nitrogen sources for growth.
  • Selective agents inhibit classes of background flora to enrich for target groups.
  • Carbohydrate(s) plus pH indicator(s) reveal acid production by fermenters via colony and/or medium color change.
  • Agar provides a porous, hydrated gel that allows diffusion of nutrients and metabolic byproducts while maintaining discrete colonies.

Note: No clinical or diagnostic claims are made. Suitability for specific organisms should be established by users through internal growth-promotion and selectivity testing per their application.

Buffer Applications

Buffer preparation is not a typical application for this product. The medium is intended to be dissolved in water (per label instructions) and solidified with agar for microbiological plating.

• If buffered conditions are required, follow the manufacturer’s preparation directions. Do not add extraneous buffering salts unless validated for your method, as they may alter the selectivity/differential behavior of the medium (literature/general).

Green Alternatives

As a water-based, agar-solidified medium, this product already avoids volatile organic solvents. Environmental considerations center on energy use, waste minimization, and responsible sterilization.

Greener practice options (literature/general):

• Autoclaving efficiency: Batch media preparation to maximize autoclave load utilization. Use validated liquid cycles that avoid overprocessing, which can degrade indicators and waste energy.
• Right-sizing: Prepare only the volume needed for the immediate study to reduce expired plate disposal.
• Reusable glassware: Where appropriate, use reusable borosilicate bottles and glass media tubes instead of single-use plastics, balancing sterility and safety requirements.
• Waste management: Autoclave used plates, then segregate and recycle plastic where permitted by institutional biosafety policy; neutralize disinfectants properly.

Comparison notes (general):

• Versus solvent-based workflows, agar media eliminate VOC emissions and flammable liquids.
• Powdered media minimize shipping mass and water transport compared to pre-poured plates; however, pre-poured plates can reduce autoclave energy use on-site—choose based on your facility’s footprint and capacity.

Pharmaceutical Uses

This product is intended for research use only. It is not offered as a pharmaceutical excipient or for clinical/diagnostic use.

Relevant contexts in pharmaceutical microbiology (literature/general):

• Growth media are commonly used for environmental monitoring, bioburden testing, or microbial method development. Any use in regulated settings requires suitability testing and vendor documentation (CoA, SDS) and may require compendial or in-house validated media formulations. This specific product’s compliance status is Not specified for this item; refer to CoA/Spec Sheet.
• For GMP applications, users typically perform growth-promotion testing with compendial strains and maintain traceability of media lots and preparation records.

Physical Properties

Item-specific physicochemical specifications are not provided in the Product Data for this mixture.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet. (Literature: dehydrated agar media are typically free-flowing, tan to beige powders.)
• Solubility: Not specified for this item; refer to CoA/Spec Sheet. (Literature: dispersible in water; dissolves upon heating to near boiling; forms a firm gel on cooling due to agar.)
• pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet. (Literature: many selective/differential agar media target near-neutral pH when prepared as directed.)
• Density/bulk density: Not specified for this item; refer to CoA/Spec Sheet.
• Melting/gelation behavior: Not specified for this item; refer to CoA/Spec Sheet. (Literature: agar gels typically melt ~85–95 C and set ~32–40 C; exact values depend on formulation and concentration.)
• Refractive index, logP, BP/MP: Not applicable/meaningful for a complex hydrated gel system; not determined.

Practical notes (general):

• Clarity, color, and gel strength of the poured plates depend on water quality, dissolution/boil step, and accurate weighing (literature).

Quality and Grades

• Grade: BioReagent (from Product Data). This designation indicates suitability for biological and microbiological research workflows where consistent performance and low bioburden are important. It is not a pharmacopeial grade and is intended for research use only.

How this typically translates in practice (literature/general):

• Composition control: Lot-to-lot consistency of nutrient and selective components to support reproducible growth/differentiation responses.
• Performance testing: Vendors commonly conduct growth-promotion/inhibition testing with representative strains, gel strength checks, clarity/color metrics, and pH of prepared medium. Specific organisms and acceptance criteria are vendor-defined. Not specified for this item; refer to CoA/Spec Sheet.
• Additives/stabilizers: Not specified for this item; refer to CoA/Spec Sheet. Indicator stability can depend on storage conditions and light exposure (general note).

What to verify upon receipt (best practice):

• Check lot-specific CoA for preparation instructions (g/L), target pH (at 25 C or 37 C), sterilization guidance, and expected appearance of the prepared plates.
• Record lot/expiry and perform internal growth-promotion verification if your quality system requires it, especially for regulated workflows.

Reaction and Applications

This product is a microbiological culture medium and is not intended for use as a reagent in synthetic organic or inorganic chemical reactions.

• Chemical reaction families (Grignard, coupling, reductions, etc.): Not applicable.

Application domain (general microbiology):

• Used to prepare solid agar plates for the isolation, differentiation, or enumeration of microorganisms consistent with BPLS-type formulations (literature/general). Specific organism claims are not provided for this item; consult the CoA/Spec Sheet and your internal validation.
• Suitable for research workflows such as routine plating, colony morphology assessment, and qualitative selection under defined incubation conditions.

Practical tips (literature/general):

• Ensure complete dissolution with gentle boiling; avoid scorching. Mix thoroughly before dispensing to achieve uniform indicator distribution.
• Degas briefly or allow to cool to ~45–50 C before pouring to minimize bubbles and condensation in plates.
• Dry plates in a sterile environment until surfaces are free of excess moisture prior to use to prevent colony spread/swarming.

Reaction Conditions

Chemical reaction conditions are not applicable. For laboratory preparation of the medium (general microbiology practice):

• Solvent: Purified/deionized water only.
• Dissolution: Suspend the dehydrated medium in water and heat to near boiling with frequent agitation until fully dissolved. Avoid localized overheating.
• Sterilization: Follow label/CoA. Many agar media are sterilized by autoclaving (e.g., 121 C, liquid cycle); however, the exact time/temperature depends on formulation and fill volume. Not specified for this item; refer to CoA/Spec Sheet.
• Pouring temperature: Allow to cool to approximately 45–50 C before pouring plates to minimize condensation and preserve indicator fidelity (literature/general).
• Incubation: Conditions (temperature, atmosphere, time) are application-dependent. Establish per your organism(s) of interest and method validation.

These are general guidelines. Adhere to the lot-specific directions supplied with the product.

Safety and Handling

Authoritative safety information is provided in the SDS. The Product Data do not list GHS classifications for this item.

• GHS/CLP status: Not specified for this item; refer to SDS. Complex dehydrated media often are not classified as hazardous, but components (e.g., bile salts, indicators) may cause irritation (literature/general).
• Signal word, hazard statements, pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (literature/general):

• PPE: Wear lab coat, safety glasses, and suitable gloves when handling the dehydrated powder and hot media.
• Dust control: Avoid creating dust/aerosols; use local exhaust or handle in a clean area to minimize inhalation/contamination.
• Thermal hazards: Hot, recently sterilized media can cause burns; handle flasks and bottles with heat-resistant gloves.
• Incompatibilities: Avoid strong oxidizers contacting the dehydrated mixture. Prepare with deionized water only; do not add solvents.
• Sterilization: Follow label/CoA guidance. If autoclaving is recommended, do not over-autoclave indicators that may degrade (literature/general).
• Microbiological safety: After use, treat plates/broths as biohazardous. Autoclave or use approved disinfectants before disposal. Prevent environmental release of cultured organisms.

First aid (general): Rinse skin/eyes with water if contacted; move to fresh air if dust inhaled; seek medical attention if irritation persists. Defer to SDS for detailed measures.

Solvent Selection

This is a water-based microbiological medium and is not used with organic solvents.

• Primary solvent: Purified/deionized water (laboratory grade). Do not substitute organic solvents.
• Polarity/miscibility: Not applicable; medium is formulated to dissolve in water upon heating and to form a gel upon cooling (literature/general).

Practical guidance:

• Use freshly produced DI/RO water with low conductivity and low total organic carbon to minimize haze and maintain indicator performance (general best practice).
• Avoid buffers or salts not prescribed by the manufacturer; they can alter selectivity and gel strength.

Comparison to alternatives (general):

• For non-selective recovery or enumeration, a neutral nutrient agar may be chosen instead of a selective/differential BPLS-type medium.
• For highly selective enrichment or different organism groups, choose media validated for those targets (e.g., bile salt–containing media, carbohydrate-differential media, or other selective agars as appropriate).

Storage and Reconstitution

From Product Data:

• Storage conditions (dehydrated medium): Room temperature in a tightly closed container.
• Shipping: Normal.
• Research use: For research use only.

Best practices (literature/general):

• Keep container tightly sealed with desiccant if provided. Store in a cool, dry place away from direct light to preserve indicator integrity.
• Avoid humid environments; moisture uptake can lead to clumping and alter performance.
• Do not refrigerate below ambient if condensation risk exists upon warm-up.

Reconstitution/Preparation:

• Use freshly produced DI/RO water. Weigh the lot-specific grams per liter indicated on the label (Not specified for this item; refer to CoA/Spec Sheet).
• Dissolve with heat and sterilize per CoA instructions. If instructed to filter-sterilize additives (e.g., antibiotics, heat-labile indicators), cool base medium to ~45–50 C before aseptic addition.
• Store poured plates inverted at 2–8 C in sealed sleeves or containers to minimize dehydration if your SOP permits; equilibrate to room temperature and dry surface condensation before use (literature/general).

Shelf life:

• Lot-specific expiry and recommended use-by dates for both dehydrated medium and prepared plates are Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

This product is a formulated microbiological medium, not a single defined chemical entity. As such, classical single-compound identifiers do not apply.

• Product type: Dehydrated culture medium (agar-based), modified BPLS formulation (mixture)
• Molecular formula: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• CAS, InChIKey, SMILES: Not applicable to complex media; Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (descriptive): Typically comprises a gelling agent (agar) plus nutrient and selective/differential components (literature). In 2D conceptual terms, the product is a blend of polysaccharide gel matrix with salts, peptones, and indicators as relevant to the BPLS concept (composition varies by manufacturer; see CoA/Spec Sheet).

Notes on terminology (literature/general):

• “BPLS” in microbiology commonly denotes a bile salt–containing, lactose/sucrose–differential base with peptones and selectivity agents; “Modified” indicates a vendor-specific optimization. This description is provided as general background only and is not a specification for this item.

Synthetic Utility

Not applicable. This product is a complex biological medium and does not serve as a reagent or building block in chemical synthesis.

• No roles in named organic reactions, catalysis, or polymer synthesis are associated with agar-based microbiological media (literature/general).

Target Specificity

Target specificity parameters (e.g., for antibodies or molecular probes) are not applicable to a culture medium.

• Organism selectivity claims and performance characteristics for this Modified BPLS Agar are Not specified for this item; refer to CoA/Spec Sheet and perform internal growth-promotion/selectivity studies for your use case (literature/general).