MSH6 Mouse mAb - Anticuerpo primario, specific to MSH6, Mouse IgG

Cat. No.: Ab116116

CALIDAD & PUREZA Carrier Free, Azide Free, Validated, 1.0 mg/mL

Application

IHC

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG

Clone Number

YN01136m

Conjugation

Unconjugated

Descripción general

Mouse anti Human MSH6 Antibody, Monoclonal (YN01136m), could be used for IHC and so on.

Application

• IHC: 1/200

Protein Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.

Especificaciones

Product Name

MSH6 Mouse mAb - Anticuerpo primario, specific to MSH6, Mouse IgG

Sinónimos

MSH6_HUMAN antibody | mutS (E. coli) homolog 6 antibody | MutS alpha 160 kDa subunit antibody | MutS homolog 6 (E. coli) antibody | mutS homolog 6 antibody | MutS-alpha 160 kDa subunit antibody | p160 antibody | Sperm associated protein antibody | DNA mis

Especificaciones y pureza

Azide Free, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Ratón

Especificidad

MSH6

Inmunógeno

Peptide

Control positivo

IHC: Human colon cancer tissue.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, Validated

Isotipo

Mouse IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

IHC: MSH6 Mouse mAb (Ab116116) ​

MSH6 Mouse mAb (Ab116116) - IHC
Immunohistochemistry analysis of paraffin-embedded human colorectal cancer tissue labelling MSH6 with MSH6 Mouse mAb (Ab116116) at 1/200 dilution. The section was incubated with Ab116116 for 30 mins at room temperature. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.​

Aplicación

Aplicación

Dilution info

IHC

1/200

Objetivos asociados (humanos)

MSH6 Tbio DNA mismatch repair protein Msh6 (0 Activities)

Discover Target: MSH6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MSH6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.