GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
NADPH oxidase (NAO) is a typical membrane protein that catalyzes the oxidation of NADPH to NADP⁺ while transferring electrons to oxygen to generate superoxide anions. It is widely distributed in animals, plants, and fungi. Dysfunction of this enzyme can lead to chronic granulomatous disease (CGD) in humans, while in plants, it is closely associated with disease resistance and various stress responses.
NADPH oxidase (NAO) converts NADPH to NADP⁺ and simultaneously generates superoxide anions (O₂⁻), which subsequently react with a chromogenic agent to form a water-soluble yellow product. Background values are excluded by adding the specific inhibitor diphenyleneiodonium (DPI) as a control. The activity of NAO is calculated by measuring the absorbance of the resulting colored product at 450 nm.
Specifications
Especificaciones y pureza
BioReagent
Grado
BioReagent
Aplicación
Enzyme activity assay
Método de detección
Colorimetric Method
Detection instrument
Microplate reader
Detection wavelength
450nm
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light
Enviado en
Wet ice
Estabilidad y almacenamiento
Store at 2-8℃ for 3 months. Store in the dark.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
How should this product be stored?
Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.