NQO1 Mouse mAb - Anticuerpo primario, specific to Human NQO1

Cat. No.: Ab224768

GRADE & PURITY Carrier Free, Validated, Endotoxin Free, ExactAb™, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Clone Number

AB02/1F11

Conjugation

Unconjugated

Descripción general

Mouse anti Human NQO1 Antibody, clone AB01/1F11, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

NQO1 Mouse mAb - Anticuerpo primario, specific to Human NQO1

Sinónimos

Azoreductase antibody | Cytochrome b 5 reductase antibody | DHQU antibody | DIA 4 antibody | DIA4 antibody | Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody | Diaphorase (NADH/NADPH) antibody | Diaphorase 4 antibody | Dioxin inducible 1 antibo

Especificaciones y pureza

Carrier Free,Endotoxin Free,ExactAb™,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofac

Especies hospedadoras

Ratón

Especificidad

Human NQO1

Inmunógeno

Recombinant full length protein corresponding human NQO1

Control positivo

WB: HepG2, MCF-7, HeLa and A549 whole cell lysates Flow Cytometry: MCF-7 cells IF/ICC: MCF-7 cells

Conjugación

Unconjugated

Grado

Carrier Free, Endotoxin Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: NQO1 Mouse mAb (Ab224768)

NQO1 Mouse mAb (Ab224768) - Western Blot
All lanes: NQO1 Mouse Antibody (Ab224768) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 30 kDa
Exposure time: 30.0 s 

Flow Cytometry: NQO1 Mouse mAb (Ab224768)

NQO1 Mouse mAb (Ab224768) - Flow Cytometry ​
Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab224768) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).​

IF: NQO1 Mouse mAb (Ab224768)

NQO1 Mouse mAb (Ab224768) - IF
IF analysis of NQO1 (Green) in MCF-7 cells. The cells were fixed with 4% PFA for 15 minutes and then 0.3% Triton X-100 for 15 minutes at RT. Cells were stained with NQO1 Mouse mAb (Ab224768) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/200;

• WB:

  1/1000 - 1/200000;

• Flow Cytometry:

  1/1000;

Objetivos asociados (humanos)

NQO1 Tchem NAD(P)H dehydrogenase [quinone] 1 (0 Activities)

Discover Target: NQO1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Endotoxin Free mean?

Endotoxin Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (NQO1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.