NruI

Cat. No.: L398135

GRADE & PURITY Suitable for molecular biology, BioReagent, PCR Reagent, for DNA synthesis, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 20 U/μl

Descripción general

Storage buffer: 10mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 200µg/ml Recombinant Albumin, 50% Glycerol, pH 7.4

NruI can recognize the TCG/CGA palindromic sequence, and form a flat terminal after enzyme digestion. The isozymes of NRUI include Bsp68I, BtuMI and RruI. Recombinant albumin was added to both NruI and 10×Cut Reaction Buffer to ensure the safety and stability of the product.

Product composition

L398135	Component	1 KU	5 KU	Storage
L398135A	NruI	50 μL	250 μL	-20°C. Avoid freeze/thaw cycle
L398135B	10×Cut Reaction Buffer	400 μL	2 mL	-20°C. Avoid freeze/thaw cycle

Source
E.coli

Restriction Enzyme CuttingSite
5’…TCG↓CGA…3’
3’…AGC↑GCT…5’ 

Enzyme Activity Definition

In a total reaction system of 50 μl, the amount of enzyme required for enzyme digestion of 1 μg λDNA within 1 h at 37℃ was defined as one active single site (U).
Protocol
(1) Configure the reaction system, as shown in the following table

Component	volume
RNase-free Water	To 50 μl
10×Cut Reaction Buffer	5 μl
DNA	1 μg
NruI	1 μl*

(2) Gently mix, do not oscillate the vortex, and then instantaneous centrifugation.
(3) Incubation at 37°C for 30 min.
Note: *Nrul is finally added to the system, generally 50 μl reaction system to add 1μl of Nrul, can also be adjusted according to the experiment, it is recommended that the amount of enzyme added does not exceed 10% of the total system to avoid star activity.
Cautions
(1) Methylation sensitivity of Nrul:
Dam methylation: Methylation and cleavage sites may overlap resulting in impaired cleavage
Dcm methylation: insensitive
CpG methylation: Blocking cleavage
(2) Before the Meche reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, so as not to affect the effect of enzyme digestion.
(3) This product is for scientific research only and shall not be used for other purposes.

Specifications

Product Name

NruI

Sinónimos

NruI | Bsp68I | BtuMI | RruI

Grado

BioReagent, for DNA and RNA applications, for DNA synthesis, For In Vitro Transcription, PCR Reagent, Suitable for molecular biology, EnzymoPure™

Especificaciones y pureza

BioReagent, PCR Reagent, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 20 U/μl

Tipo de molécula

Enzima

Almacenamiento y envío

Concentración

20 U/μl

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Preguntas frecuentes

What does PCR Reagent mean?

PCR Reagent indicates that the product has been processed and tested for PCR, sequencing and nucleic acid amplification. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.