tablet, To prepare 5 mL for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)
to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay
as a substrate for ExtrAvidin alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)
as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
Specifications
Product Name
p-Nitrophenyl phosphate Tablets
Especificaciones y pureza
tablet, To prepare 5 mL
Tipo de molécula
Molécula pequeña
Almacenamiento y envío
Concentración
tablet, To prepare 5 mL
Condiciones de almacenamiento de almacenamiento
Store at -20°C
Enviado en
Ice chest + Ice pads
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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