p53 Mouse mAb - Anticuerpo primario, specific to Human p53

Cat. No.: Ab169235

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Clone Number

EF03/6B12

Conjugation

Unconjugated

Descripción general

Mouse anti Human p53 Antibody, clone EF03/6B12, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name

p53 Mouse mAb - Anticuerpo primario, specific to Human p53

Sinónimos

p53 induced gene 11 protein antibody | p53 induced protein antibody | PIG 11 antibody | PIG11 antibody | Tumor protein p53 inducible protein 11 antibody | Anti- p53 | Mouse Anti-human p53 | Monoclonal Anti- p53 antibody | p53 Mouse Monoclonal Antibody | p

Especificaciones y pureza

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circum

Especies hospedadoras

Ratón

Especificidad

Human p53

Inmunógeno

Recombinant protein corresponding to Human p53

Control positivo

WB: HEK293 and A431 whole cell lysates Flow Cytometry: HEK293 cells

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: p53 Mouse mAb (Ab169235)

p53 Mouse mAb (Ab169235) - Western Blot
All lanes: p53 Mouse Antibody (Ab169235) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 52 kDa
Exposure time: 30.0 s

Flow Cytometry: p53 Mouse mAb (Ab169235)

p53 Mouse mAb (Ab169235) - Flow Cytometry ​
Flow Cytometry analysis of HEK293 cells stained with p53  Mouse mAb (Ab169235) (Red) at 2.5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).​

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 - 1/20000 ;

• Flow Cytometry:

  1/200 - 1/3;

Objetivos asociados (humanos)

TP53 Tchem Cellular tumor antigen p53 (0 Activities)

Discover Target: TP53

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (p53 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.