P53R3 - ≥99% , CAS No.922150-12-7

CAS: 922150-12-7 Cat. No.: P647644 Fórmula: C₃₂H₃₅Cl₂N₅O₂ Peso molecular: 592.56 PubChem CID: 58809185

GRADE & PURITY ≥99%

Storage

Store at 2-8°C

Shipped In

Wet ice

Descripción general

P53R3 is a potent p53 reactivator and restores sequence-specific DNA binding of p53 hot spot mutants, including p53 R175H , p53 R248W and p53 R273H. P53R3 induces p53-dependent antiproliferative effects with much higher specificity than PRIMA-1. P53R3 enhances the recruitment of wild-type p53 and p53 M237I to several target gene promoters. P53R3 strongly enhances the mRNA, total protein and cell surface expression of the death receptor death receptor 5 (DR5). P53R3 is used for cancer research.

In Vitro

P53R3 (10 μg/ml; 24 hours; in the absence or presence of the unlabelled p53 consensus oligonucleotide) restores p53-specific DNA binding activity to p53 R273H (a DNA contact mutant) and p53R175H (a structural mutant) in WiDr colon tumour cells harbouring p53 R273H and KLE cells with p53R175H. P53R3 (1-33 μg/ml; 24 hours) inhibits the proliferation of the LN-308 sublines expressing mutant p53 plasmids in a p53-dependent manner. The p53 R175H -dependent effects are strong over a broad range of concentrations, but p53 R273H -dependent effects are weaker and requires high concentrations of P53R3. P53R3 induces p53 R248W reactivation is more pronounced proliferation inhibition than observed with p53 R273H . P53R3 does not exhibit cytotoxic effects even at concentrations close to its solubility limit (33 μg/ml). P53R3 (33 μg/ml; 18 hours) induces a strong decrease in S phase cells and a G0/G1 cell cycle arrest in LN-308 p53 R175H and LN-308 p53 R273H cells. But it does not affect cell cycle distribution of LN-308 p53 R248W cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: p53 LN-308 human glioma cells with a control plasmid or plasmids encoding the mutants p53R175H, p53R248W and p53R273H Concentration: 1-33 μg/mL Incubation Time: 24 hours Result: Induced p53-dependent and -independent antiproliferative and cytotoxic effects in vitro .

Form:Solid

Specifications

Especificaciones y pureza

≥99%

Mecanismos bioquímicos y fisiológicos

P53R3 is a potent p53 reactivator and restores sequence-specific DNA binding of p53 hot spot mutants, including p53 R175H , p53 R248W and p53 R273H . P53R3 induces p53-dependent antiproliferative effects with much higher specificity than PRIMA-1. P53R3 en

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Pureza

≥99%

Nombres e identificadores

• Sonrisas canónicas: CC(C)[C@@H](C(=O)OC)NC1=NC(=NC2=CC=CC=C21)CN3CCN(CC3)C(C4=CC=C(C=C4)Cl)C5=CC=C(C=C5)Cl
• Isómeros SMILES: CC(C)[C@@H](C(=O)OC)NC1=NC(=NC2=CC=CC=C21)CN3CCN(CC3)C(C4=CC=C(C=C4)Cl)C5=CC=C(C=C5)Cl
• PubChem CID: 58809185
• Peso molecular: 592.56

Propiedades químicas y físicas

• Solubilidad: DMSO : ≥ 100 mg/mL (168.76 mM)

Preguntas frecuentes

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 922150-12-7, the molecular formula is C32H35Cl2N5O2, and the molecular weight is 592.56 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.